Construct: ORF TRCN0000478011
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010212.1_s317c1
- Derived from:
- ccsbBroadEn_03485
- DNA Barcode:
- CAGCGGAAAGTCAGCTAAGTACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IMPAD1 (54928)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478011
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54928 | IMPAD1 | inositol monophosphatase do... | NM_017813.5 | 100% | 100% | |
| 2 | human | 54928 | IMPAD1 | inositol monophosphatase do... | XR_928786.2 | 14.6% | 1_289del;936_1044del;1476_7340del | |
| 3 | mouse | 242291 | Impad1 | inositol monophosphatase do... | NM_177730.3 | 89.1% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1143
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccccatgggc atccgccttt ccccactggg ggtggcagtg ttttgcctgc 121 tggggctcgg cgtgctctac cacctctact cgggcttctt ggccggccgc ttcagcctct 181 tcggcctggg cggcgagcct ggcggcggcg cggcggggcc cgcggccgcg gccgatgggg 241 gcaccgtgga cttgcgcgag atgctggctg tgtcagtgct ggccgcagtc cgcggcggcg 301 acgaggtgag gcgcgtccgc gagagcaacg tcctccacga gaagtccaag gggaagacgc 361 gcgagggagc cgaggacaag atgaccagcg gcgacgtgct gtccaaccgc aagatgttct 421 acctgctcaa gaccgccttc cccagcgtcc agattaatac tgaggaacac gtggatgcag 481 ctgatcagga ggttatcttg tgggatcata agattcctga ggatatccta aaggaagtaa 541 ctactcctaa agaggtacca gcagaaagtg ttactgtctg gattgaccca cttgatgcta 601 cacaggaata tacagaggat cttcgaaagt acgtcactac TATGGTGTGT GTGGCTGTAA 661 ATGGTAAACC CATGCTAGGA GTTATACATA AGCCATTTTC CGAATATACA GCTTGGGCAA 721 TGGTAGATGG TGGTTCAAAT GTGAAAGCCC GCTCTTCCTA CAATGAGAAG ACCCCAAGGA 781 TCGTTGTGTC TCGTTCCCAT TCAGGGATGG TCAAACAGGT CGCTCTTCAG ACTTTTGGAA 841 ACCAGACTAC AATTATCCCA GCTGGTGGTG CTGGTTATAA AGTTTTAGCA CTTTTGGATG 901 TGCCTGATAA GAGTCAAGAA AAAGCTGATT TATACATCCA TGTGACATAC ATCAAAAAGT 961 GGGATATATG TGCTGGTAAT GCCATCTTAA AAGCCCTAGG GGGGCATATG ACTACCCTGA 1021 GTGGTGAAGA AATCAGTTAC ACTGGTTCAG ACGGCATTGA AGGGGGACTC CTTGCTAGCA 1081 TCAGAATGAA CCACCAGGCC CTGGTCAGAA AACTCCCAGA TCTAGAAAAG ACAGGACATA 1141 AATGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGACA GCGGAAAGTC AGCTAAGTAC GAACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt