Construct: ORF TRCN0000478013
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017862.1_s317c1
- Derived from:
- ccsbBroadEn_05573
- DNA Barcode:
- TGTGGCGTTCTCAATCAAGCTGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INSYN1 (388135)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478013
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 388135 | INSYN1 | inhibitory synaptic factor 1 | NM_001039614.2 | 99.8% | 100% | 357T>C |
| 2 | human | 388135 | INSYN1 | inhibitory synaptic factor 1 | XM_005254369.4 | 99.8% | 100% | 357T>C |
| 3 | human | 388135 | INSYN1 | inhibitory synaptic factor 1 | NM_001303254.1 | 90.3% | 90.4% | 0_1ins84;273T>C |
| 4 | mouse | 319477 | 6030419C18Rik | RIKEN cDNA 6030419C18 gene | NM_176921.3 | 86.5% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 948
- ORF length:
- 879
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacattcgg ggcgccccgg acctcgggca gcccagtgac gaccccagca 121 gtggtggtga gcgggagcgg attcgacagc gcatgaagat ggtcatcggg cagcttgagg 181 gcatccttcg cgagctcaag gaggtggcca aggagctgag ggaggtggtg agccagatcg 241 ataagctaac ctcggacttc gactttgaac tggagccgga cgactggacc acggccactg 301 tgagcagcac ctctagcagt gacaaggcgg gcatgggtgg cccctttgac ctgggccacc 361 tggacttcat gacagccgat atcctctcag acagctggga gttctgctcc ttcctggacg 421 tctccacccc ctcggactcc gtggatggtc ccgagtcgac tcggccaggg gctggccctg 481 actaccgcct catgaatggt ggcacgccca tccccaatgg gccacgagtg gagaccccag 541 actcctccag tgaggaggcc tttggtgctg gccccacggt gaagagccag ctgccccagc 601 ggaccccagg gacacgggag agggtgcggt tcagtgacaa agtgctctac catgctctgt 661 gctgtgacga tgaggagggg gacggtgagc aggaggtgga ggaggaagaa gtgggcttgc 721 ccccTGAGCC TGCCCATACA GAGGCCCATG CAGGCCCCCA CAAGCCCTCC CCAGCCCCCT 781 ACAAGTCACG GCGCTCTCCA CTGACCAGCC GCCACTCAGG CTCTACCTTG GCCCCTGAAC 841 AGACTCGAAG GGTCACGAGG AACAGCAGCA CCCAGACAGT GTCAGACAAG AGCACGCAGA 901 CGGTTCTGCC CTACACAGCC ACTAGACAGA AAGCCAGGGG GAAAAACTTG CCAACTTTCT 961 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1021 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1081 GTGGAAAGGA CGATGTGGCG TTCTCAATCA AGCTGTGACG CGTTAAGTCg acaatcaacc 1141 tctggattac aaaatttgtg aaagatt