Construct: ORF TRCN0000478024
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013505.1_s317c1
- Derived from:
- ccsbBroadEn_13989
- DNA Barcode:
- TTGCGATAGGTCACGCTAGTCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PABPN1 (8106)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478024
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8106 | PABPN1 | poly(A) binding protein nuc... | NM_004643.3 | 99.3% | 99% | 3_8delGGCGGC |
| 2 | human | 8106 | PABPN1 | poly(A) binding protein nuc... | NM_001360551.2 | 95.3% | 95% | 3_8delGGCGGC;883_885delTCA;888_888delAins34 |
| 3 | human | 100529063 | BCL2L2-PABPN1 | BCL2L2-PABPN1 readthrough | NM_001199864.2 | 73.4% | 50.3% | (many diffs) |
| 4 | human | 8106 | PABPN1 | poly(A) binding protein nuc... | NM_001360552.1 | 58.5% | 58.5% | 0_1ins378 |
| 5 | mouse | 54196 | Pabpn1 | poly(A) binding protein, nu... | NM_019402.2 | 92.7% | 95.7% | (many diffs) |
| 6 | mouse | 54196 | Pabpn1 | poly(A) binding protein, nu... | XM_006519272.3 | 55% | 57.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 978
- ORF length:
- 912
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcggcg gcagcagcag cgggggctgc gggcggtcgg ggctccgggc 121 cggggcggcg gcgccatctt gtgcccgggg ccggtgggga ggccggggag ggggccccgg 181 ggggcgcagg ggactacggg aacggcctgg agtctgagga actggagcct gaggagctgc 241 tgctggagcc cgagccggag cccgagcccg aagaggagcc gccccggccc cgcgcccccc 301 cgggagctcc gggccctggg cctggttcgg gagcccccgg cagccaagag gaggaggagg 361 agccgggact ggtcgagggt gacccggggg acggcgccat tgaggacccg gagctggaag 421 ctatcaaagc tcgagtcagg gagatggagg aagaagctga gaagctaaag gagctacaga 481 acgaggtaga gaagcagatg aatatgagtc caccTCCAGG CAATGCTGGC CCGGTGATCA 541 TGTCCATTGA GGAGAAGATG GAGGCTGATG CCCGTTCCAT CTATGTTGGC AATGTGGACT 601 ATGGTGCAAC AGCAGAAGAG CTGGAAGCTC ACTTTCATGG CTGTGGTTCA GTCAACCGTG 661 TTACCATACT GTGTGACAAA TTTAGTGGCC ATCCCAAAGG GTTTGCGTAT ATAGAGTTCT 721 CAGACAAAGA GTCAGTGAGG ACTTCCTTGG CCTTAGATGA GTCCCTATTT AGAGGAAGGC 781 AAATCAAGGT GATCCCAAAA CGAACCAACA GACCAGGCAT CAGCACAACA GACCGGGGTT 841 TTCCACGAGC CCGCTACCGC GCCCGGACCA CCAACTACAA CAGCTCCCGC TCTCGATTCT 901 ACAGTGGTTT TAACAGCAGG CCCCGGGGTC GCGTCTACAG GGGCCGGGCT AGAGCGACAT 961 CATGGTATTC CCCTTACTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1021 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAGA 1081 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTGCGAT AGGTCACGCT 1141 AGTCCTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt