Construct: ORF TRCN0000478041
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000662.1_s317c1
- Derived from:
- ccsbBroadEn_14215
- DNA Barcode:
- CATTATCGTAACAATCCTTGAAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL4 (56062)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478041
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56062 | KLHL4 | kelch like family member 4 | NM_019117.5 | 99.9% | 99.4% | 1359C>T;2144_2145insG |
| 2 | human | 56062 | KLHL4 | kelch like family member 4 | NM_057162.3 | 97.2% | 95.6% | (many diffs) |
| 3 | human | 56062 | KLHL4 | kelch like family member 4 | XR_938403.2 | 73.2% | (many diffs) | |
| 4 | mouse | 237010 | Klhl4 | kelch-like 4 | NM_172781.2 | 86.1% | 87.4% | (many diffs) |
| 5 | mouse | 237010 | Klhl4 | kelch-like 4 | NM_001290477.1 | 85.5% | 86.7% | (many diffs) |
| 6 | mouse | 237010 | Klhl4 | kelch-like 4 | NM_001290478.1 | 82.3% | 84% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2226
- ORF length:
- 2157
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcagtgtct ggcaagaaag agtttgatgt gaaacagatc ctaaggctac 121 gctggaggtg gtttagtcat ccttttcaag gttccaccaa cactggaagc tgtcttcagc 181 aggaaggata tgagcataga gggaccccgg ttcagggcag gttgaagagc cactctcggg 241 acagaaacgg actgaagaaa agcaacagtc ctgtccacca caatatactg gcaccagtgc 301 caggaccggc ccctgcccat cagagagccg ttcagaattt gcagcaacat aatctgatag 361 tacattttca agcaaatgaa gatactccta aatcagttcc agagaagaat ttattcaaag 421 aagcttgtga gaaacgcgca caagatttgg agatgatggc tgatgacaat atagaagatt 481 ctacagcaag attagataca caacactctg aagacatgaa tgccaccaga tctgaagagc 541 agttccatgt tataaaccac gcagagcaaa ctcttcgtaa aatggagaac tacttgaaag 601 agaaacaact atgtgatgtg ctactgattg caggacacct ccgcatccca gcccataggt 661 tggttctcag cgcagtgtct gattattttg ctgcaatgtt tactaatgat gtgcttgaag 721 ccaaacaaga agaggtcagg atggaaggag tagatccaaa tgcactaaat tccttggtgc 781 agtatgctta cacaggagtc ctgcaattga aagaagatac cattgaaagt ttgctggctg 841 cagcttgtct tctgcagctg actcaggtca ttgatgtttg ctccaatttt ctcataaagc 901 agctccatcc ttcaaactgc ttagggattc gatcatttgg agatgcccaa ggctgtacag 961 aacttctgaa cgtggcacac aaatacacta tggaacactt cattgaggta ataaaaaacc 1021 aagaattcct cctgcttcca gctaatgaaa tttcaaaact tctgtgcagt gatgacatta 1081 atgtgcctga tgaagagacc atttttcatg ctctaatgca gtgggtgggg catgatgtgc 1141 agaataggca aggagaactg gggatgctgc tttcttacat cagactgcca ttactcccac 1201 cacagttact ggcagatctt gaaaccagtt ccatgtttac tggtgatctt gagtgtcaga 1261 agctcctgat ggaagctatg aagtatcatc ttttgcctga gagaagatcc atgatgcaaa 1321 gccctcggac aaagcctaga aaatcaactg tgggggcact ttatgctgta ggaggcatgg 1381 atgctatgaa aggtactact actattgaaa aatatgacct caggactaac agttggctac 1441 atattggcac catgaatggc cgtaggcttc aatttggagt cgcagttatt gataataagc 1501 tctatgtcgt gggaggaaga gacggtttaa aaactttgaa tacagtggaa tgttttaatc 1561 cagttggcaa aatctggact gtgatgcctc ccatgtcaac acatcggcac ggcttaggtg 1621 tagccactct tgaaggacca atgtatgctg taggtggtca tgatggatgg agctatctaa 1681 atactgtaga aagatgggac cctgagggac gacagtggaa ttacgtagcc agtatgtcaa 1741 ctcctagaag cacagttggt gttgttgcat taaacaacaa aTTATATGCT ATTGGTGGAC 1801 GTGATGGAAG TTCCTGCCTC AAATCAATGG AATACTTTGA CCCACACACT AACAAGTGGA 1861 GTTTGTGTGC TCCAATGTCC AAAAGACGTG GAGGTGTGGG AGTTGCCACA TACAATGGAT 1921 TCTTATATGT TGTAGGGGGG CATGATGCCC CTGCTTCCAA CCATTGCTCC AGGCTTTCTG 1981 ACTGTGTGGA ACGGTATGAT CCAAAAGGTG ATTCATGGTC AACTGTGGCA CCTCTGAGTG 2041 TTCCTCGAGA TGCTGTTGCT GTGTGCCCTC TTGGAGACAA ACTCTACGTG GTTGGAGGAT 2101 ATGACGGACA TACTTATTTG AACACAGTTG AGTCATATGA TGCACAGAGA AATGAATGGA 2161 AAGAGGAAGT TCCTGTTAAC ATTGGAAGAG CTGGTGCATG TGTTGTAGTG GTGGAAGCTA 2221 CCCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC 2281 CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT 2341 TGGCTTTATA TATCTTGTGG AAAGGACGAC ATTATCGTAA CAATCCTTGA AGTACGCGTT 2401 AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att