Construct: ORF TRCN0000478043
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011299.1_s317c1
- Derived from:
- ccsbBroadEn_09468
- DNA Barcode:
- GCCCATCTCTAATTTGCTTACGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPH2 (121278)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478043
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 121278 | TPH2 | tryptophan hydroxylase 2 | NM_173353.4 | 99.9% | 99.7% | 158G>A |
| 2 | mouse | 216343 | Tph2 | tryptophan hydroxylase 2 | NM_173391.3 | 87.3% | 92.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1539
- ORF length:
- 1470
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagccagca atgatgatgt tttccagtaa atactgggca cggagagggt 121 tttccctgga ttcagcagtg cccgaagagc atcagctact tggcagctca acactaaata 181 aacctaactc tggcaaaaat gacgacaaag gcaacaaggg aagcaacaaa cgtgaagctg 241 ctaccgaaag tggcaagaca gcagttgttt tctccttgaa gaatgaagtt ggtggattgg 301 taaaagcact gaggctcttt caggaaaaac gtgtcaacat ggttcatatt gaatccagga 361 aatctcggcg aagaagttct gaggttgaaa tctttgtgga ctgtgagtgt gggaaaacag 421 aattcaatga gctcattcag ttgctgaaat ttcaaaccac tattgtgacg ctgaatcctc 481 cagagaacat ttggacagag gaagaagagc tagaggatgt gccctggttc cctcggaaga 541 tctctgagtt agacaaatgc tctcacagag ttctcatgta tggttctgag cttgatgctg 601 accacccagg atttaaggac aatgtctatc gacagagaag aaagtatttt gtggatgtgg 661 ccatgggtta taaatatggt cagcccattc ccagggtgga gtatactgaa gaagaaacta 721 aaacttgggg tgttgtattc cgggagctct ccaaactcta tcccactcat gcttgccgag 781 agtatttgaa aaacttccct ctgctgacta aatactgtgg ctacagagag gacaatgtgc 841 ctcaactcga agatgtctcc atgtttctga aagaaaggtc tggcttcacg gtgaggccgg 901 tggctggata cctgagccca cgagactttc tggcaggact ggcctacaga gtgttccact 961 gtacccagta catccggcat ggctcagatc ccctctacac cccagaacca gacacatgcc 1021 atgaactctt gggacatgtt ccactacttg cggatcctaa gtttgctcag ttttcacaag 1081 aaataggTCT GGCGTCTCTG GGAGCATCAG ATGAAGATGT TCAGAAACTA GCCACGTGCT 1141 ATTTCTTCAC AATCGAGTTT GGCCTTTGCA AGCAAGAAGG GCAACTGCGG GCATATGGAG 1201 CAGGACTCCT TTCCTCCATT GGAGAATTAA AGCACGCCCT TTCTGACAAG GCATGTGTGA 1261 AAGCCTTTGA CCCAAAGACA ACTTGCTTAC AGGAATGCCT TATCACCACC TTCCAGGAAG 1321 CCTACTTTGT TTCAGAAAGT TTTGAAGAAG CCAAAGAAAA GATGAGGGAC TTTGCAAAGT 1381 CAATTACCCG TCCCTTCTCA GTATACTTCA ATCCCTACAC ACAGAGTATT GAAATTCTGA 1441 AAGACACCAG AAGTATTGAA AATGTGGTGC AGGACCTTCG CAGCGACTTG AATACAGTGT 1501 GTGATGCTTT AAACAAAATG AACCAATATC TGGGGATTTT GCCAACTTTC TTGTACAAAG 1561 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1621 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1681 ACGAGCCCAT CTCTAATTTG CTTACGCGAC GCGTTAAGTC gacaatcaac ctctggatta 1741 caaaatttgt gaaagatt