Construct: ORF TRCN0000478065

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006509.1_s317c1
Derived from:
ccsbBroadEn_06246
DNA Barcode:
TTAATCTACTGCCGCTTGACGACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GABRG2 (2566)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478065
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2566 GABRG2 gamma-aminobutyric acid typ... NM_000816.3 99.9% 100% 588C>T
2 human 2566 GABRG2 gamma-aminobutyric acid typ... NM_198904.3 98.2% 98.3% 588C>T;1129_1152del
3 human 2566 GABRG2 gamma-aminobutyric acid typ... NM_198903.2 90.6% 90.4% 588C>T;632_751del;1249_1272del
4 mouse 14406 Gabrg2 gamma-aminobutyric acid (GA... NM_177408.6 90.7% 99.1% (many diffs)
5 mouse 14406 Gabrg2 gamma-aminobutyric acid (GA... NM_008073.3 89.2% 97.4% (many diffs)
6 mouse 14406 Gabrg2 gamma-aminobutyric acid (GA... XM_006532199.1 85.5% 93.6% (many diffs)
7 mouse 14406 Gabrg2 gamma-aminobutyric acid (GA... XM_006532200.2 85.4% 93.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1470
ORF length:
1401
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gagttcgcca aatatatgga gcacaggaag ctcagtctac tcgactcctg
121tattttcaca gaaaatgacg gtgtggattc tgctcctgct gtcgctctac cctggcttca
181ctagccagaa atctgatgat gactatgaag attatgcttc taacaaaaca tgggtcttga
241ctccaaaagt tcctgagggt gatgtcactg tcatcttaaa caacctgctg gaaggatatg
301acaataaact tcggcctgat ataggagtga agccaacgtt aattcacaca gacatgtatg
361tgaatagcat tggtccagtg aacgctatca atatggaata cactattgat atattttttg
421cgcaaacgtg gtatgacaga cgtttgaaat ttaacagcac cattaaagtc ctccgattga
481acagcaacat ggtggggaaa atctggattc cagacacttt cttcagaaat tccaaaaaag
541ctgatgcaca ctggatcacc acccccaaca ggatgctgag aatttggaat gatggtcgag
601tgctctacac cctaaggttg acaattgatg ctgagtgcca attacaattg cacaattttc
661caatggatga acactcctgc cccttggagt tctccagtta tggctatcca cgtgaagaaa
721ttgtttatca atggaagcga agttctgttg aagtgggcga cacaagatcc tggaggcttt
781atcaattctc atttgttggt ctaagaaata ccaccgaagt agtgaagaca acttccggag
841attatgtggt catgtctgtc tactttgatc tgagcagaag aatgggatac tttaccatcc
901agacctatat cccctgcaca ctcattgtcg tcctatcctg ggtgtctttc tggatcaata
961aggatgctgt tccagccaga acatctttag gtatcaccac tgtcctgaca atgaccaccc
1021tcagcaccat tgcccggaaa tcgctcccca aggtctccta tgtcacagcg atggatctct
1081ttgtatctgt ttgtttcatc tttgtcttct ctgctctggt ggagtatggc accttgcatt
1141attttgtcag caaccggaaa ccaagcaagg acaaagataa aaagaagaaa aaccctgccc
1201cTACCATTGA TATCCGCCCA AGATCAGCAA CCATTCAAAT GAATAATGCT ACACACCTTC
1261AAGAGAGAGA TGAAGAGTAC GGCTATGAGT GTCTGGACGG CAAGGACTGT GCCAGTTTTT
1321TCTGCTGTTT TGAAGATTGT CGAACAGGAG CTTGGAGACA TGGGAGGATA CATATCCGCA
1381TTGCCAAAAT GGACTCCTAT GCTCGGATCT TCTTCCCCAC TGCCTTCTGC CTGTTTAATC
1441TGGTCTATTG GGTCTCCTAC CTCTACCTGT TGCCAACTTT CTTGTACAAA GTGGTTGATA
1501TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1561CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTAAT
1621CTACTGCCGC TTGACGACAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1681tgaaagatt

Download FASTA (ORF) (Full)