Transcript: Mouse NM_177408.6

Mus musculus gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 (Gabrg2), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Gabrg2 (14406)
Length:
3911
CDS:
385..1785

Additional Resources:

NCBI RefSeq record:
NM_177408.6
NBCI Gene record:
Gabrg2 (14406)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_177408.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000089093 CCGTGGTTATTGTAATTTGAA pLKO.1 1945 3UTR 100% 5.625 7.875 N Gabrg2 n/a
2 TRCN0000089097 CAGATCAGCAACCATTCAAAT pLKO.1 1533 CDS 100% 13.200 10.560 N Gabrg2 n/a
3 TRCN0000089096 CCTCGTGAAGAAATTGTTTAT pLKO.1 1021 CDS 100% 13.200 9.240 N Gabrg2 n/a
4 TRCN0000434007 GAGACATGGGAGGATACATAT pLKO_005 1668 CDS 100% 13.200 9.240 N GABRG2 n/a
5 TRCN0000089095 CTATGAAGATTACGCTTCTAA pLKO.1 516 CDS 100% 5.625 3.938 N Gabrg2 n/a
6 TRCN0000089094 GCAACCATTCAAATGAACAAT pLKO.1 1540 CDS 100% 5.625 3.938 N Gabrg2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_177408.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06246 pDONR223 100% 90.7% 99.1% None (many diffs) n/a
2 ccsbBroad304_06246 pLX_304 0% 90.7% 99.1% V5 (many diffs) n/a
3 TRCN0000478065 TTAATCTACTGCCGCTTGACGACA pLX_317 19.5% 90.7% 99.1% V5 (many diffs) n/a
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