Construct: ORF TRCN0000478066
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011501.1_s317c1
- Derived from:
- ccsbBroadEn_00897
- DNA Barcode:
- TTGCCTTCCAGAGATTTTCTGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNJ14 (3770)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478066
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3770 | KCNJ14 | potassium inwardly rectifyi... | NM_013348.4 | 100% | 100% | |
| 2 | mouse | 211480 | Kcnj14 | potassium inwardly-rectifyi... | NM_145963.2 | 88.1% | 94.2% | (many diffs) |
| 3 | mouse | 211480 | Kcnj14 | potassium inwardly-rectifyi... | XM_017322094.1 | 88.1% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1374
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cctggccagg gccctacgcc gcctcagcgg cgccctggat tcgggagaca 121 gccgggcggg cgatgaagag gaggccgggc ccgggttgtg ccgcaacggg tgggcgccgg 181 caccggtgca gtcacccgtg ggccggcgcc gcggtcgctt cgtcaagaaa gacgggcact 241 gcaacgtgcg tttcgtaaac ctgggtggcc agggcgcgcg ctacctgagc gacctgttca 301 ccacatgcgt ggacgtgcgc tggcgctgga tgtgcctgct cttctcctgc tccttcctcg 361 cctcctggct gctcttcggc ctggccttct ggctcattgc ctcgctgcac ggcgacctgg 421 ccgccccgcc accgcccgcg ccctgcttct cacacgtggc cagcttcctg gccgccttcc 481 tcttcgcgct ggagacgcag acgtccatcg gctacggcgt gcgcagcgtc accgaggagt 541 gcccggccgc tgtggccgcc gtggtgctgc agtgcattgc cggctgcgtg ctcgacgcct 601 tcgtcgtggg tgctgtcatg gccaagatgg ccaaacccaa gaagcgcaac gagacgctgg 661 tcttcagcga gaacgccgtc gtggcgctgc gcgaccaccg cctctgcctc atgtggcgcg 721 tcggcaacct gcgccgcagc cacctggtcg aggcccacgt gcgtgcccag ctgctgcagc 781 cccgtgtgac cccagagggt gagtacatcc cgctggacca ccaggatgtg gatgtgggct 841 ttgatggagg caccgatcgt atcttcctcg tgtcccccat caccatcgtc catgagatcg 901 actctgccag tcctctgtat gagctaggac gtgccgagct ggccagggct gactttgagc 961 tggtggtcat tctcgagggg atggttgagg ccacagccat gaccacacag tgtcgctcgt 1021 cctacctccc tggtgaactg ctctggggcc atcgttttga gccagttctc ttccagcgtg 1081 gctcccagta tgaggtcgac tatcgccact tccatcgcac ttatgaggtc ccagggacac 1141 cggtctgcag tgctaaggag ctggatgaac gggcagagca ggcttcccac agcctcaagt 1201 ctagtttccc cggctctctg actgcatttt gttatgagaa tgaacttgct cTGAGCTGCT 1261 GCCAGGAGGA AGATGAGGAC GATGAGACTG AGGAAGGGAA TGGGGTGGAA ACAGAAGATG 1321 GGGCTGCTAG CCCCCGAGTT CTCACACCAA CCCTGGCGCT GACCCTGCCT CCATGCCCAA 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAT TGCCTTCCAG AGATTTTCTG TTTACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att