Construct: ORF TRCN0000478089
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005194.1_s317c1
- Derived from:
- ccsbBroadEn_03910
- DNA Barcode:
- GGCATTCCTTTCCGGGGTCTCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCUR1 (63933)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478089
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63933 | MCUR1 | mitochondrial calcium unipo... | NM_001031713.3 | 100% | 100% | |
| 2 | human | 63933 | MCUR1 | mitochondrial calcium unipo... | XM_011514802.1 | 95.9% | 93.9% | (many diffs) |
| 3 | human | 63933 | MCUR1 | mitochondrial calcium unipo... | XR_001743580.1 | 29.8% | 1_565del;979_1080del;1188_1189ins556 | |
| 4 | human | 63933 | MCUR1 | mitochondrial calcium unipo... | XR_001743579.2 | 20% | 1_134del;989_1079del;1303_5381del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1143
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctgcggctcg gtcggcggcc agaggaccca gcgcctgccc ggccgccagc 121 ggcttctctt cttgcccgtg ggcctcagcg gaagaccggg cggcagcgaa acctcagcac 181 gccgctgcct ctctgcgctc tccgacggtc tgggggcgct gcgccctcgc gccccggcgg 241 cccgcggcgg cgtgtcacgt gcctcaccgc tgctcctcct cctgctagtg ccctccccgc 301 gcttggccgc cgcagccccg cgccggcagc tcggggactg ggagcgctcg cgcctcgggt 361 atgccgcacc cccggccggg cgcagcagcg cgtggaggtg ctcaccgggc gtcgccgctg 421 ccgccggcgc ccttccccag taccacggcc cggcgcctgc actcgtttcc tgcaggagag 481 agctaagctt gtctgctgga tccctgcagc tggagcgcaa aaggagagat ttcacctctt 541 ctgggagcag gaaactctac ttcgacactc atgccttagt gtgcttactg gaagacaatg 601 ggtttgctac tcaacaagca gaaatcattg tgtctgcatt ggtcaagatc ctggaggcca 661 acatggacat cgtctacaaa gatatggtca ccaagatgca gcaggaaatc acttttcagc 721 aagtaatgtc tcagattgcg aatgtgaaaa aggatatgat tattttggag aagagtgaat 781 tttcagccct cagagcagaa aatgagaaaa taaaactcga actacatcag ttaaaacaac 841 aagtaatgga tgaagtgatc aaagtccgaa cagataccaa attagacttc aacctagaaa 901 agagcagagt aaaagaattg tattcattga acgaaaagaa gctgctggaa ttgagaacag 961 aaatagtggc attgcatgcc cagcaagatc gggcccttac CCAGACAGAC AGGAAGATCG 1021 AAACTGAGGT TGCTGGCCTC AAAACCATGC TTGAGTCACA CAAGCTTGAT AATATTAAAT 1081 ATTTAGCAGG GTCTATATTT ACGTGCCTAA CAGTAGCTCT GGGATTTTAT CGCCTGTGGA 1141 TCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGAGG CATTCCTTTC CGGGGTCTCC CAACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt