Construct: ORF TRCN0000478090
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014657.1_s317c1
- Derived from:
- ccsbBroadEn_01415
- DNA Barcode:
- CGTAGCTGACATATCATTGGGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPE65 (6121)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478090
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6121 | RPE65 | retinoid isomerohydrolase R... | NM_000329.3 | 100% | 100% | |
| 2 | human | 6121 | RPE65 | retinoid isomerohydrolase R... | XM_017002027.1 | 82.7% | 82.7% | 0_1ins276 |
| 3 | mouse | 19892 | Rpe65 | retinal pigment epithelium 65 | NM_029987.2 | 89.9% | 93.6% | (many diffs) |
| 4 | mouse | 19892 | Rpe65 | retinal pigment epithelium 65 | XM_006501165.2 | 89.7% | 93.4% | (many diffs) |
| 5 | mouse | 19892 | Rpe65 | retinal pigment epithelium 65 | XR_001783668.1 | 40.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1668
- ORF length:
- 1599
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctatccag gttgagcatc ctgctggtgg ttacaagaaa ctgtttgaaa 121 ctgtggagga actgtcctcg ccgctcacag ctcatgtaac aggcaggatc cccctctggc 181 tcaccggcag tctccttcga tgtgggccag gactctttga agttggatct gagccatttt 241 accacctgtt tgatgggcaa gccctcctgc acaagtttga ctttaaagaa ggacatgtca 301 cataccacag aaggttcatc cgcactgatg cttacgtacg ggcaatgact gagaaaagga 361 tcgtcataac agaatttggc acctgtgctt tcccagatcc ctgcaagaat atattttcca 421 ggtttttttc ttactttcga ggagtagagg ttactgacaa tgcccttgtt aatgtctacc 481 cagtggggga agattactac gcttgcacag agaccaactt tattacaaag attaatccag 541 agaccttgga gacaattaag caggttgatc tttgcaacta tgtctctgtc aatggggcca 601 ctgctcaccc ccacattgaa aatgatggaa ccgtttacaa tattggtaat tgctttggaa 661 aaaatttttc aattgcctac aacattgtaa agatcccacc actgcaagca gacaaggaag 721 atccaataag caagtcagag atcgttgtac aattcccctg cagtgaccga ttcaagccat 781 cttacgttca tagttttggt ctgactccca actatatcgt ttttgtggag acaccagtca 841 aaattaacct gttcaagttc ctttcttcat ggagtctttg gggagccaac tacatggatt 901 gttttgagtc caatgaaacc atgggggttt ggcttcatat tgctgacaaa aaaaggaaaa 961 agtacctcaa taataaatac agaacttctc ctttcaacct cttccatcac atcaacacct 1021 atgaagacaa tgggtttctg attgtggatc tctgctgctg gaaaggattt gagtttgttt 1081 ataattactt atatttagcc aatttacgtg agaactggga agaggtgaaa aaaaatgcca 1141 gaaaggctcc ccaacctgaa gttaggagat atgtacttcc tttgaatatt gacaaggctg 1201 acacaggcaa gaatttagtc acgctcccca atacaactgc cactgcaatt ctgtgcagtg 1261 acgagactat ctggctggag cctgaagttc tcttttcagg gcctcgtcaa gcatttgagt 1321 ttcctcaaat caattaccag aagtattgtg ggaaaccTTA CACATATGCG TATGGACTTG 1381 GCTTGAATCA CTTTGTTCCA GATAGGCTCT GTAAGCTGAA TGTCAAAACT AAAGAAACTT 1441 GGGTTTGGCA AGAGCCTGAT TCATACCCAT CAGAACCCAT CTTTGTTTCT CACCCAGATG 1501 CCTTGGAAGA AGATGATGGT GTAGTTCTGA GTGTGGTGGT GAGCCCAGGA GCAGGACAAA 1561 AGCCTGCTTA TCTCCTGATT CTGAATGCCA AGGACTTAAG TGAAGTTGCC CGGGCTGAAG 1621 TGGAGATTAA CATCCCTGTC ACCTTTCATG GACTGTTCAA AAAATCTTTG CCAACTTTCT 1681 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1741 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1801 GTGGAAAGGA CGACGTAGCT GACATATCAT TGGGGCAACG CGTTAAGTCg acaatcaacc 1861 tctggattac aaaatttgtg aaagatt