Construct: ORF TRCN0000478110
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005006.1_s317c1
- Derived from:
- ccsbBroadEn_12477
- DNA Barcode:
- AAAACCCTTACACCGGTATCAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMPRSS3 (64699)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478110
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64699 | TMPRSS3 | transmembrane serine protea... | NM_001256317.2 | 99.9% | 100% | 453G>A |
| 2 | human | 64699 | TMPRSS3 | transmembrane serine protea... | NM_024022.3 | 99.7% | 99.7% | 453G>A;1047_1049delAGC |
| 3 | human | 64699 | TMPRSS3 | transmembrane serine protea... | NM_032405.2 | 74% | 69.3% | (many diffs) |
| 4 | human | 64699 | TMPRSS3 | transmembrane serine protea... | NM_032404.2 | 71.7% | 71.8% | 0_1ins381;72G>A;666_668delAGC |
| 5 | mouse | 140765 | Tmprss3 | transmembrane protease, ser... | NM_080727.2 | 84.7% | 88.9% | (many diffs) |
| 6 | mouse | 140765 | Tmprss3 | transmembrane protease, ser... | NM_001163776.1 | 80.8% | 84.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1428
- ORF length:
- 1359
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggaaaat gatccgcctg ctgttgaagc ccccttctca ttccgatcgc 121 tttttggcct tgatgatttg aaaataagtc ctgttgcacc agatgcagat gctgttgctg 181 cacagatcct gtcactgctg ccattgaagt tttttccaat catcgtcatt gggatcattg 241 cattgatatt agcactggcc attggtctgg gcatccactt cgactgctca gggaagtaca 301 gatgtcgctc atcctttaag tgtatcgagc tgatagctcg atgtgacgga gtctcggatt 361 gcaaagacgg ggaggacgag taccgctgtg tccgggtggg tggtcagaat gccgtgctcc 421 aggtgttcac agctgcttcg tggaagacca tgtgctccga tgactggaag ggtcactacg 481 caaatgttgc ctgtgcccaa ctgggtttcc caagctatgt aagttcagat aacctcagag 541 tgagctcgct ggaggggcag ttccgggagg agtttgtgtc catcgatcac ctcttgccag 601 atgacaaggt gactgcatta caccactcag tatatgtgag ggagggatgt gcctctggcc 661 acgtggttac cttgcagtgc acagcctgtg gtcatagaag gggctacagc tcacgcatcg 721 tgggtggaaa catgtccttg ctctcgcagt ggccctggca ggccagcctt cagttccagg 781 gctaccacct gtgcgggggc tctgtcatca cgcccctgtg gatcatcact gctgcacact 841 gtgtttatga cttgtacctc cccaagtcat ggaccatcca ggtgggtcta gtttccctgt 901 tggacaatcc agccccatcc cacttggtgg agaagattgt ctaccacagc aagtacaagc 961 caaagaggct gggcaatgac atcgccctta tgaagctggc cgggccactc acgttcaatg 1021 aaatgatcca gcctgtgtgc ctgcccaact cTGAAGAGAA CTTCCCCGAT GGAAAAGTGT 1081 GCTGGACGTC AGGATGGGGG GCCACAGAGG ATGGAGGTGA CGCCTCCCCT GTCCTGAACC 1141 ACGCGGCCGT CCCTTTGATT TCCAACAAGA TCTGCAACCA CAGGGACGTG TACGGTGGCA 1201 TCATCTCCCC CTCCATGCTC TGCGCGGGCT ACCTGACGGG TGGCGTGGAC AGCTGCCAGG 1261 GGGACAGCGG GGGGCCCCTG GTGTGTCAAG AGAGGAGGCT GTGGAAGTTA GTGGGAGCGA 1321 CCAGCTTTGG CATCGGCTGC GCAGAGGTGA ACAAGCCTGG GGTGTACACC CGTGTCACCT 1381 CCTTCCTGGA CTGGATCCAC GAGCAGATGG AGAGAGACCT AAAAACCTTG CCAACTTTCT 1441 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1501 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1561 GTGGAAAGGA CGAAAAACCC TTACACCGGT ATCAACTACG CGTTAAGTCg acaatcaacc 1621 tctggattac aaaatttgtg aaagatt