Construct: ORF TRCN0000478124
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001360.1_s317c1
- Derived from:
- ccsbBroadEn_09571
- DNA Barcode:
- TGACCACTGTCTTATGATCCGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOT1L1 (137362)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478124
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 137362 | GOT1L1 | glutamic-oxaloacetic transa... | NM_152413.3 | 99.9% | 99.7% | 668A>G |
| 2 | human | 137362 | GOT1L1 | glutamic-oxaloacetic transa... | XM_005273399.3 | 99.6% | 99.5% | 517_518insAGC;665A>G |
| 3 | human | 137362 | GOT1L1 | glutamic-oxaloacetic transa... | XM_006716285.3 | 92.5% | 92.3% | 517_518ins93;575A>G |
| 4 | human | 137362 | GOT1L1 | glutamic-oxaloacetic transa... | XM_024447059.1 | 56.6% | 56.5% | 0_1ins546;122A>G |
| 5 | human | 137362 | GOT1L1 | glutamic-oxaloacetic transa... | XR_949376.2 | 53% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1329
- ORF length:
- 1263
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc caccctttca gtgttcatgg atgtgcccct cgcccacaag ctagagggca 121 gcttgttaaa gacctacaaa caagatgatt acccgaacaa gatattctta gcctatagag 181 tctgcatgac aaatgaaggc catccctggg tttctctcgt ggtgcagaag actcgactac 241 agatttcaca ggatccctcc ctgaattatg agtacttgcc caccatgggc ctgaaatcat 301 tcatccaggc ctctctagca ctcctctttg gaaagcacag ccaagccatt gtggagaaca 361 gggtaggggg tgtacacact gttggtgaca gtggtgcctt ccagcttggc gtccagtttc 421 tcagagcttg gcataaggat gctcgtatag tttacatcat ctcttctcaa aaagaactgc 481 atggactcgt cttccaggac atgggcttta cagtttatga atactctgtc tgggacccca 541 agaagctatg catggacccc gacatactcc tcaatgtggt ggagcagatc ccacatggct 601 gtgtccttgt gatggggaac attatcgact gcaagttgac accaagtggg tgggcaaagt 661 tgatgtccat gataaagagc aagcagatat tcccattttt tgatattccc tgtcaaggtt 721 tatacaccag tggcttggaa gaagatacta gaatcttaca atactttgtg tctcaaggct 781 ttgagttctt ctgcagccag tctctgtcca agaattttgg catttatgat gaaggagtgg 841 ggatgctagt ggtggtggca gtcaacaacc agcagctgct gtgtgtcctc tcccagctgg 901 aaggattagc ccaggccctg tggctaaacc cccccaacac gggtgcacgt gtcatcacct 961 ccatcctctg caaccctgct ctgctgggag aatggaagca gagtctaaaa gaagttgtag 1021 agaacatcat gctaaccaag gaaaaagtga aggagaaact ccagctcctg ggaacccctg 1081 ggtcctGGGG TCACATCACC GAGCAGAGTG GGACCCACGG CTATCTTGGA CTCAACTCCC 1141 AGCAGGTGGA ATACCTGGTC AGGAAGAAGC ACATCTATAT CCCCAAGAAC GGTCAGATTA 1201 ACTTCAGCTG TATCAATGCC AACAACATAA ATTACATCAC TGAGGGCATC AATGAGGCTG 1261 TCCTCCTCAC AGAGAGCTCA GAGATGTGTC TTCCAAAGGA AAAAAAAACA CTGATTGGAA 1321 TAAAACTTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1381 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1441 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGACCA CTGTCTTATG ATCCGACGAC 1501 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt