Construct: ORF TRCN0000478126
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002596.1_s317c1
- Derived from:
- ccsbBroadEn_11664
- DNA Barcode:
- TGCCAAGACCTCGTCTTCATATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF21B (23046)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478126
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23046 | KIF21B | kinesin family member 21B | NM_001252102.2 | 100% | 100% | |
| 2 | human | 23046 | KIF21B | kinesin family member 21B | NM_001252103.2 | 99.1% | 99.1% | 3803_3804ins39 |
| 3 | human | 23046 | KIF21B | kinesin family member 21B | NM_001252100.2 | 98.8% | 98.2% | (many diffs) |
| 4 | human | 23046 | KIF21B | kinesin family member 21B | NM_017596.4 | 98% | 97.4% | (many diffs) |
| 5 | human | 23046 | KIF21B | kinesin family member 21B | XM_017000731.1 | 95.4% | 94.8% | (many diffs) |
| 6 | human | 23046 | KIF21B | kinesin family member 21B | XM_017000732.1 | 74.3% | 73.6% | (many diffs) |
| 7 | mouse | 16565 | Kif21b | kinesin family member 21B | NM_001039472.1 | 88.7% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4938
- ORF length:
- 4869
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccggccag ggggactgct gcgtcaaggt ggccgtcagg atccggcccc 121 agctgtcgaa ggagaagatt gagggctgtc acatctgtac ctctgttacc ccgggagagc 181 cccaggtcct gctggggaag gacaaggcct tcacctatga ctttgtcttc gacctggaca 241 cctggcaaga acagatctat tccacctgtg tgagcaagct catcgagggc tgcttcgagg 301 gctataatgc cacggtgctg gcctatgggc agacgggggc cgggaagacg tacaccatgg 361 gcactggctt tgacatggca acgtcggagg aggagcaggg catcatcccg agggccatcg 421 cacacctctt tgggggcatt gccgagcgca agcgccgggc acaggagcag ggcgtggctg 481 gacctgagtt caaagtcagc gcccagtttc tggagctcta caacgaggag atccttgacc 541 tgtttgacag cacccgtgac cctgacaccc gccaccgcag gtccaacatc aagatccacg 601 aggacgcaaa cggtggcatc tacaccactg gcgtcacttc tcgcctcatc cactcccagg 661 aggagctgat ccagtgcctg aagcaggggg ccctgtcccg caccacagcc agcacccaga 721 tgaacgtgca gagctcacgc tcccacgcca tcttcaccat ccacctgtgc cagatgcgca 781 tgtgcaccca gcccgacctg gtgaatgagg cggtgactgg gcttcctgat ggtacacctc 841 cctcgagtga gtatgagaca ctcactgcta agtttcactt tgtggacctg gccggctcag 901 agcggctgaa gcggacaggg gctactggcg agcgggccaa ggagggcatc tccatcaact 961 gtggcctgct ggccttgggc aatgtgatca gcgccttagg ggaccagagc aagaaggtgg 1021 tgcacgttcc ctacagggac tccaagctca ctcggctcct ccaggattcg ctggggggca 1081 acagccagac catcatgatc gcctgtgtga gcccctcaga ccgagatttc atggagaccc 1141 tcaacacact caaatatgcc aatcgggccc gcaacatcaa gaacaaggtg gtagtgaacc 1201 aggacaagac cagccagcaa atcagtgcac tgcgggctga gattgctcgg ctgcagatgg 1261 agctgatgga gtataaggcg ggcaagcgag tgataggaga ggatggcgct gagggctata 1321 gtgatctgtt ccgagagaat gccatgctac agaaggagaa tggggccctg cggctgcggg 1381 tgaaagccat gcaggaggcc atcgatgcca tcaacaaccg cgtcacccag ctcatgagcc 1441 aggaggccaa cctgctgcta gccaaggccg gcgatggcaa tgaggccatt ggtgcgctga 1501 tccagaacta catccgggag atcgaggagc tacggactaa gcttctagag agtgaagcca 1561 tgaacgagtc cctgcgccgc agcctctcac gggcctcggc taggagcccc tactccctgg 1621 gtgcttctcc agccgccccg gccttcgggg gcagccctgc cagctccatg gaggatgcct 1681 cggaggtgat ccgcagggcc aagcaggacc tggagcggct aaagaagaag gaggtcaggc 1741 agcggaggaa gagccccgag aaggaagcct tcaaaaagag ggcaaaactc caacaggaga 1801 acagcgagga gacggatgag aacgaggcgg aggaggagga ggaagagcga gacgagagtg 1861 gctgtgagga ggaggaaggg cgcgaggatg aagatgagga ctcgggcagt gaagagagcc 1921 tggtggactc agactcagac cccgaggaga aggaggtgaa cttccaggcg gacctggccg 1981 acctgacttg tgagatcgaa atcaagcaga agctgatcga cgagctggag aacagccagc 2041 ggcggttgca gacgctcaag caccagtatg aggaaaagct gattctgctg cagaacaaga 2101 tccgagacac acagctggag cgcgaccgtg tgctgcagaa cctcagcacc atggagtgct 2161 atactgagga gaaggccaac aagatcaagg cagactatga gaagaggctg cgggagatga 2221 accgggacct gcagaagctg caggccgccc agaaagagca cgcccggctg cttaagaacc 2281 agtcgcgcta cgagagggag ctgaagaagc tacaggccga ggtggctgag atgaagaagg 2341 ccaaggtggc cctgatgaag cagatgcgtg aggagcaaca gcggcggcgg ctagtggaga 2401 ccaagaggaa ccgggagatc gcacagctca agaaggagca gcggcgacag gagtttcaga 2461 tccgagctct ggagtcccag aagcggcagc aggagatggt cctgaggagg aagacccagg 2521 aggtttctgc actgaggcgc ctggccaagc ccatgtctga gcgggtggca gggcgtgcag 2581 gactaaagcc acccatgctg gactctgggg ctgaggtgtc ggccagcact acctcatctg 2641 aggctgaatc aggggcccgc tctgtctcca gcatcgtgcg ccagtggaac cgcaaaatca 2701 accacttctt gggggaccat cctgcgccca ctgtcaatgg cacccgtcct gcccgaaaga 2761 agttccagaa gaagggggcc agccagagct tcagtaaggc ggcaaggctc aagtggcagt 2821 ccctggagcg acggatcatt gacatcgtca tgcagagaat gaccattgtc aacctggagg 2881 ctgacatgga gcggctcatc aagaaaaggg aggagctgtt cctcctgcag gaggcactgc 2941 ggaggaagcg ggagcggctg caggctgaga gccccgagga agagaagggg ctgcaggagc 3001 tggctgagga gatcgaggtg ctggcagcca acattgacta catcaatgac ggcatcaccg 3061 actgccaggc caccatcgtg cagctggagg agaccaagga ggagctggac tccacagaca 3121 catccgtggt catcagctcc tgctccctgg ctgaagcccg cctcctgcta gacaacttcc 3181 tcaaggcatc cattgacaag gggctgcaag tggcacaaaa ggaagcccag atccggctgt 3241 tggagggccg actgaggcag acggatatgg caggctcctc ccagaaccat ctgctcctgg 3301 acgccctgcg tgagaaggct gaagctcacc ccgagctgca ggccctcatc tacaatgtgc 3361 agcaggagaa tggctacgcc agcacagatg aggagatctc agagttctct gagggcagct 3421 tctcccagtc attcaccatg aaaggctcca ccagccatga cgatttcaag ttcaagagcg 3481 agcccaaact gtctgcccaa atgaaagctg tgtcggctga gtgcctgggc cccccactgg 3541 atatctccac caagaacatc accaagtccc tggcctccct cgttgagatc aaagaggacg 3601 gagtgggctt ctctgtccga gacccctatt accgggacag ggtctcgcgc accgtcagtc 3661 tgcctacccg gggcagcact ttccctaggc aatctcgagc cacagagacg tccccgctga 3721 cgagaaggaa gtcctacgac cgagggcagc ccattaggtc cacagatgtg ggattcacac 3781 ccccatcatc ccctcccact cggccccgca atgaccgcaa tgtcttctct cgtctcacca 3841 gtaatcagag ccaggggtca gcgctggaca agtctgatga cagcgactcc tctttgtcgg 3901 aggtcctgag gggcatcatc tccccggttg gaggagccaa gggtgcacgg acggccccac 3961 tgcagtgtgt ctccatggcc gaaggccaca ccaagcccat cctctgcctg gatgccacag 4021 atgagttgct attcacaggg tccaaagacc gaagctgcaa gatgtggaac ttggttacgg 4081 gacaggagat cgcagctcta aagggccacc ccaacaacgt ggtctccatc aagtactgca 4141 gccactcggg gcttgtgttc tccgtgtcca cctcctacat caaggtgtgg gacatccggg 4201 actcagccaa gtgcattcgg actctcacgt cctcgggcca ggtgatctca ggggatgcct 4261 gtgccgccac atccacccgt gccatcacca gtgctcaggg cgagcatcag atcaaccaga 4321 tcgccctcag cccttcgggc accatgctgt acgccgcctc gggcaatgcc gtccgcatct 4381 gggagcttag caggttccag cctgtcggca agctgactgg ccacatcggc cctgtgatgt 4441 gcctgacggt cacccagacg gccagccagc atgacctcgt ggtgactggc tccaaggacc 4501 actacgttaa gatgttcgag ctgGGCGAGT GTGTGACGGG CACCATCGGC CCCACTCACA 4561 ACTTCGAGCC CCCGCACTAC GATGGCATCG AGTGTCTCGC CATCCAGGGA GACATCCTGT 4621 TCAGTGGCTC CCGAGATAAC GGCATCAAGA AGTGGGACCT AGACCAGCAG GAGCTCATCC 4681 AGCAAATCCC CAATGCGCAC AAGGACTGGG TGTGCGCCCT GGCCTTCATC CCGGGCCGCC 4741 CCATGCTGCT CAGCGCCTGC CGTGCGGGTG TCATCAAGGT CTGGAACGTG GACAACTTCA 4801 CACCCATCGG TGAGATCAAG GGCCACGACA GTCCCATCAA TGCCATCTGC ACCAATGCCA 4861 AGCATATCTT CACAGCCTCC AGTGACCTGA CGGTGAAGTT CTGGAGTGTC CGGCGGTTAC 4921 CCCACAGCGG CCCACCCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 4981 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 5041 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGCCAAG ACCTCGTCTT 5101 CATATTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt