Construct: ORF TRCN0000478132
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001635.1_s317c1
- Derived from:
- ccsbBroadEn_10187
- DNA Barcode:
- GAACACGAATGGATGCGCCGATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INSYN2A (642938)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478132
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 642938 | INSYN2A | inhibitory synaptic factor 2A | NM_001039762.3 | 99.9% | 100% | 39G>A |
| 2 | human | 642938 | INSYN2A | inhibitory synaptic factor 2A | XM_005252694.4 | 99.9% | 100% | 39G>A |
| 3 | human | 642938 | INSYN2A | inhibitory synaptic factor 2A | XM_017016537.2 | 99.9% | 100% | 39G>A |
| 4 | human | 642938 | INSYN2A | inhibitory synaptic factor 2A | XM_017016538.2 | 99.9% | 100% | 39G>A |
| 5 | human | 642938 | INSYN2A | inhibitory synaptic factor 2A | XM_017016539.2 | 99.9% | 100% | 39G>A |
| 6 | human | 642938 | INSYN2A | inhibitory synaptic factor 2A | XM_017016540.2 | 99.9% | 100% | 39G>A |
| 7 | human | 642938 | INSYN2A | inhibitory synaptic factor 2A | XM_017016541.1 | 99.9% | 100% | 39G>A |
| 8 | human | 642938 | INSYN2A | inhibitory synaptic factor 2A | XM_017016542.2 | 99.9% | 100% | 39G>A |
| 9 | human | 642938 | INSYN2A | inhibitory synaptic factor 2A | XM_017016543.1 | 83.5% | 81.8% | (many diffs) |
| 10 | mouse | 627214 | Fam196a | family with sequence simila... | NM_001143802.1 | 81.5% | 83.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1506
- ORF length:
- 1437
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtcagtaag gacaccggca aatgcatact cacaacatcg gagagtgaag 121 tggaacccgc cgcctgcctg gccctggaga tgaaatacgc cctggacccc aaccggcaga 181 ttaaaaaacg gaacaaagcc ctgcaggtgc ggtttaagga tatctgcgag gcacagaatg 241 agcagaggga cacacagctg tcctcgggcc agctggggga gaagcgggag gccaagcccg 301 tgtcctgcag agcagcctac cgcaaataca tgacagtgcc cgcacgcagg tccatcccca 361 acgtcaccaa gagcacaggc gtgcagacct cgcccgacct taagaagtgt taccagacgt 421 tccctctgga ccgcaaaaag gggaacctca aaagcctccc agctgcagat ccctttaaaa 481 gccaaaacaa tgggtttcta acagatgcga aagagaagaa cgaggctgga cccatggagg 541 aggcccggcc atgtggcgcg gggcgggtgc acaagaccac agccttggtt ttccattcca 601 accaacacat gaacacagtg gaccagcctt tgggggtcaa ctgcacagag ccctgtaaaa 661 gcccggagcc gctcagctat ggagaagctg cgctccaaaa ctccactcgg cctccatccg 721 aagagcccga ttaccagctg ctcgggaggg ccaagcagga ccgggggagg ccaaactccg 781 aggagcccgc tccacctgcc ctcaggaggg tgtttaaaac ggaggttgcc accgtttacg 841 cacctgccct cagtgccagg gcccccgagc ctggtttgtc agactctgca gccgccagcc 901 agtggtcact ctgcccggca gatgacgagc ggaggagagc cacacatctc aacgggctcc 961 aggcgccctc ggaaactgcc ctggcctgct cacccccgat gcagtgcctg tcccccgaat 1021 gtagtgagca gccgtcgcag actcacaccc cgccggggct ggggaaccag ccTAGTCCCA 1081 CAGCGGTTGC TGCAGGTGAA GAATGCCAAC GAATCGTGCC TCATACGGAA GTGGTCGACC 1141 TCAAAGCACA ACTTCAGATG ATGGAGAACT TGATCAGTTC AAGCCAAGAA ACCATCAAAG 1201 TGCTCTTGGG GGTCATTCAG GAGTTGGAAA AAGGAGAGGC CCATCGGGAA GGGCTTTCAT 1261 ATCGGACGGG GCAAGACACA GCTAATTGTG ACACATGCAG GAACAGTGCA TGTATTATCT 1321 ATAGTGTGGA GCTGGATTTT AAGCAGCAAG AAGACAAACT CCAGCCGGTT CTAAGAAAAC 1381 TCCACCCTAT TGAGGAAACT CAGGTCATAC CTTCGCCTTA CTCTCAGGAG ACTTACTCCT 1441 CAACTCCCAA GCAAAAATCC AAAACTGAAT CTAAAAAGCA CGGAAGATGG AAACTCTGGT 1501 TCCTTTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1561 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1621 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGAACACGAA TGGATGCGCC GATACACGCG 1681 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt