Construct: ORF TRCN0000478173
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016943.1_s317c1
- Derived from:
- ccsbBroadEn_16066
- DNA Barcode:
- ATCGCACAAAGATGCCGCTACTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2Q2 (92912)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478173
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92912 | UBE2Q2 | ubiquitin conjugating enzym... | XM_017022728.2 | 27.2% | 27.2% | 1_570del |
| 2 | human | 92912 | UBE2Q2 | ubiquitin conjugating enzym... | XM_005254788.2 | 23.9% | 23.9% | 1_675del |
| 3 | human | 92912 | UBE2Q2 | ubiquitin conjugating enzym... | NM_001284382.2 | 20.8% | 20.8% | 1_807del |
| 4 | human | 92912 | UBE2Q2 | ubiquitin conjugating enzym... | NM_001145335.1 | 19.7% | 19.7% | 1_864del |
| 5 | human | 92912 | UBE2Q2 | ubiquitin conjugating enzym... | NM_173469.4 | 18.9% | 18.9% | 1_912del |
| 6 | human | 55585 | UBE2Q1 | ubiquitin conjugating enzym... | NM_017582.7 | 13.5% | 15.4% | (many diffs) |
| 7 | human | 92912 | UBE2Q2 | ubiquitin conjugating enzym... | XR_001751421.2 | 7.3% | 1_1090del;1304_2895del | |
| 8 | human | 92912 | UBE2Q2 | ubiquitin conjugating enzym... | XR_001751420.2 | 7.2% | 1_1151del;1365_2956del | |
| 9 | human | 92912 | UBE2Q2 | ubiquitin conjugating enzym... | XR_002957692.1 | 6.8% | 1_1326del;1540_3131del | |
| 10 | mouse | 109161 | Ube2q2 | ubiquitin-conjugating enzym... | NM_001346658.1 | 26.5% | 27.8% | (many diffs) |
| 11 | mouse | 109161 | Ube2q2 | ubiquitin-conjugating enzym... | NM_001346657.1 | 19.7% | 20.6% | (many diffs) |
| 12 | mouse | 109161 | Ube2q2 | ubiquitin-conjugating enzym... | NM_180600.3 | 17.9% | 18.7% | (many diffs) |
| 13 | mouse | 245350 | AA414768 | expressed sequence AA414768 | NM_001272033.1 | 16.1% | 16% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 279
- ORF length:
- 213
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga acttctcaca aaacagggct ggagcagtgc cTACTCAATA GAATCGGTCA 121 TCATGCAAAT AAATGCCACC TTAGTCAAAG GCAAAGCCAG AGTGCAGTTT GGAGCAAATA 181 AGAATCAATA TAATCTAGCA AGAGCCCAAC AATCCTATAA TTCCATTGTA CAGATACATG 241 AGAAAAATGG CTGGTACACC CCTCCAAAGG AAGATGGCTA CCCAACTTTC TTGTACAAAG 301 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 361 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 421 ACGAATCGCA CAAAGATGCC GCTACTGTAC GCGTTAAGTC gacaatcaac ctctggatta 481 caaaatttgt gaaagatt