Transcript: Human NM_001145335.1

Homo sapiens ubiquitin conjugating enzyme E2 Q2 (UBE2Q2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
UBE2Q2 (92912)
Length:
2985
CDS:
311..1390

Additional Resources:

NCBI RefSeq record:
NM_001145335.1
NBCI Gene record:
UBE2Q2 (92912)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001145335.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000415246 ATCGGTCATCATGCAAATAAA pLKO_005 1222 CDS 100% 15.000 21.000 N UBE2Q2 n/a
2 TRCN0000007755 GAGCCCAACAATCCTATAATT pLKO.1 1311 CDS 100% 15.000 12.000 N UBE2Q2 n/a
3 TRCN0000436280 GCAAGCTTCAGATAGACTTAT pLKO_005 871 CDS 100% 13.200 9.240 N UBE2Q2 n/a
4 TRCN0000007757 CCATTTGTTCGAGTGGTGTTA pLKO.1 1109 CDS 100% 4.950 3.465 N UBE2Q2 n/a
5 TRCN0000007756 CTGGATGTTGAGATGCTAGAT pLKO.1 608 CDS 100% 4.950 3.465 N UBE2Q2 n/a
6 TRCN0000007754 GCCACCTTAGTCAAAGGCAAA pLKO.1 1244 CDS 100% 4.050 2.835 N UBE2Q2 n/a
7 TRCN0000434415 ATACAGATCACAGAGTTATAA pLKO_005 910 CDS 100% 15.000 9.000 N UBE2Q2 n/a
8 TRCN0000436691 GATACTAAGAACAACAATTTG pLKO_005 524 CDS 100% 13.200 7.920 N UBE2Q2 n/a
9 TRCN0000418261 TGATAGGAAACCTACTCATTA pLKO_005 1888 3UTR 100% 13.200 7.920 N UBE2Q2 n/a
10 TRCN0000007753 GCACAAAGACTCCTTGAGCAA pLKO.1 2225 3UTR 100% 2.640 1.584 N UBE2Q2 n/a
11 TRCN0000177221 GAGGAAGAAGAAGAAGAGATA pLKO.1 683 CDS 100% 4.950 2.475 Y Cnpy4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001145335.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04593 pDONR223 100% 87.5% 83.2% None (many diffs) n/a
2 ccsbBroad304_04593 pLX_304 0% 87.5% 83.2% V5 (many diffs) n/a
3 TRCN0000469294 GACCGCAACTACCGCAAATAGGGA pLX_317 45.5% 87.5% 83.2% V5 (many diffs) n/a
4 ccsbBroadEn_16066 pDONR223 0% 19.7% 19.7% None 1_864del n/a
5 ccsbBroad304_16066 pLX_304 0% 19.7% 19.7% V5 1_864del n/a
6 TRCN0000478173 ATCGCACAAAGATGCCGCTACTGT pLX_317 80.6% 19.7% 19.7% V5 1_864del n/a
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