Construct: ORF TRCN0000478176

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000128.1_s317c1
Derived from:
ccsbBroadEn_08775
DNA Barcode:
TGATTCCTATGGTCTCCAGTCTAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SQOR (58472)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478176
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 58472 SQOR sulfide quinone oxidoreductase NM_001271213.1 99.8% 100% 12G>T;1341A>T
2 human 58472 SQOR sulfide quinone oxidoreductase NM_021199.4 99.8% 100% 12G>T;1341A>T
3 mouse 59010 Sqor sulfide quinone oxidoreductase NM_001162503.1 83.1% 86.8% (many diffs)
4 mouse 59010 Sqor sulfide quinone oxidoreductase NM_021507.5 83.1% 86.8% (many diffs)
5 mouse 59010 Sqor sulfide quinone oxidoreductase XM_006499966.1 83.1% 86.8% (many diffs)
6 mouse 59010 Sqor sulfide quinone oxidoreductase XM_006499967.1 45.2% 47.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1416
ORF length:
1350
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gccacttgtg gctgtggtat cagggccccg tgcccagctc tttgcctgcc
121tgctcaggct gggcactcag caggtcggcc cccttcagct gcacaccggg gccagccatg
181cggccaggaa ccattatgag gtgctggtgc tgggtggggg cagtggcgga atcaccatgg
241ctgcccgcat gaagaggaaa gtgggtgcag agaatgtggc cattgttgag cccagtgaga
301gacatttcta ccagccaatc tggacactgg tgggtgctgg tgccaaacaa ttgtcctcat
361ctggtcgtcc cacggcaagt gtgattccat ctggtgtaga atggatcaaa gctagagtga
421ctgagttgaa cccagacaag aactgcattc acacagatga cgacgagaag atctcctacc
481gatatcttat tattgctctc ggaatccagc tggactatga gaagattaaa ggcctacctg
541aaggtttcgc tcatcccaaa atagggtcga attattcagt taagactgta gagaagacat
601ggaaagctct gcaggacttc aaagagggca atgccatctt caccttccca aatactccag
661tgaagtgtgc tggagcccct cagaagatca tgtacttatc agaagcctac ttcaggaaga
721cagggaagcg atccaaggcc aatatcattt tcaacacttc tcttggagcc attttcgggg
781ttaagaagta tgcagatgcc ctgcaggaga tcatccagga gcggaacctc actgttaact
841acaagaaaaa cctcattgaa gtccgagccg ataaacaaga ggctgtattt gagaacctgg
901acaaaccagg agagacccaa gtgatttcat atgaaatgct tcatgtcaca cctccaatga
961gcccaccaga tgtcctcaag accagtcctg tggctgatgc tgctggttgg gtggatgtgg
1021ataaagaaac tctgcaacac aggaggtacc caaatgtgtt tgggattggg gactgcacca
1081accttcctac gtcaaagacc gctgctgcag tagctgccca gtcaggaata cttgatagga
1141caatttctgt aattatgaag aatcaaacac CAACAAAGAA GTATGATGGC TACACATCAT
1201GTCCACTGGT GACCGGCTAC AACCGTGTGA TTCTTGCTGA GTTTGACTAC AAAGCAGAGC
1261CGCTAGAAAC CTTCCCCTTT GATCAAAGCA AAGAGCGCCT TTCCATGTAT CTCATGAAAG
1321CTGACCTGAT GCCTTTCCTG TATTGGAATA TGATGCTAAG GGGTTACTGG GGAGGACCAG
1381CGTTTCTGCG CAAGTTGTTT CATCTTGGTA TGAGTTCCCC AACTTTCTTG TACAAAGTGG
1441TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1501CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1561ATGATTCCTA TGGTCTCCAG TCTACACGCG TTAAGTCgac aatcaacctc tggattacaa
1621aatttgtgaa agatt

Download FASTA (ORF) (Full)