Construct: ORF TRCN0000478176
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000128.1_s317c1
- Derived from:
- ccsbBroadEn_08775
- DNA Barcode:
- TGATTCCTATGGTCTCCAGTCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SQOR (58472)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478176
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58472 | SQOR | sulfide quinone oxidoreductase | NM_001271213.1 | 99.8% | 100% | 12G>T;1341A>T |
| 2 | human | 58472 | SQOR | sulfide quinone oxidoreductase | NM_021199.4 | 99.8% | 100% | 12G>T;1341A>T |
| 3 | mouse | 59010 | Sqor | sulfide quinone oxidoreductase | NM_001162503.1 | 83.1% | 86.8% | (many diffs) |
| 4 | mouse | 59010 | Sqor | sulfide quinone oxidoreductase | NM_021507.5 | 83.1% | 86.8% | (many diffs) |
| 5 | mouse | 59010 | Sqor | sulfide quinone oxidoreductase | XM_006499966.1 | 83.1% | 86.8% | (many diffs) |
| 6 | mouse | 59010 | Sqor | sulfide quinone oxidoreductase | XM_006499967.1 | 45.2% | 47.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1416
- ORF length:
- 1350
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gccacttgtg gctgtggtat cagggccccg tgcccagctc tttgcctgcc 121 tgctcaggct gggcactcag caggtcggcc cccttcagct gcacaccggg gccagccatg 181 cggccaggaa ccattatgag gtgctggtgc tgggtggggg cagtggcgga atcaccatgg 241 ctgcccgcat gaagaggaaa gtgggtgcag agaatgtggc cattgttgag cccagtgaga 301 gacatttcta ccagccaatc tggacactgg tgggtgctgg tgccaaacaa ttgtcctcat 361 ctggtcgtcc cacggcaagt gtgattccat ctggtgtaga atggatcaaa gctagagtga 421 ctgagttgaa cccagacaag aactgcattc acacagatga cgacgagaag atctcctacc 481 gatatcttat tattgctctc ggaatccagc tggactatga gaagattaaa ggcctacctg 541 aaggtttcgc tcatcccaaa atagggtcga attattcagt taagactgta gagaagacat 601 ggaaagctct gcaggacttc aaagagggca atgccatctt caccttccca aatactccag 661 tgaagtgtgc tggagcccct cagaagatca tgtacttatc agaagcctac ttcaggaaga 721 cagggaagcg atccaaggcc aatatcattt tcaacacttc tcttggagcc attttcgggg 781 ttaagaagta tgcagatgcc ctgcaggaga tcatccagga gcggaacctc actgttaact 841 acaagaaaaa cctcattgaa gtccgagccg ataaacaaga ggctgtattt gagaacctgg 901 acaaaccagg agagacccaa gtgatttcat atgaaatgct tcatgtcaca cctccaatga 961 gcccaccaga tgtcctcaag accagtcctg tggctgatgc tgctggttgg gtggatgtgg 1021 ataaagaaac tctgcaacac aggaggtacc caaatgtgtt tgggattggg gactgcacca 1081 accttcctac gtcaaagacc gctgctgcag tagctgccca gtcaggaata cttgatagga 1141 caatttctgt aattatgaag aatcaaacac CAACAAAGAA GTATGATGGC TACACATCAT 1201 GTCCACTGGT GACCGGCTAC AACCGTGTGA TTCTTGCTGA GTTTGACTAC AAAGCAGAGC 1261 CGCTAGAAAC CTTCCCCTTT GATCAAAGCA AAGAGCGCCT TTCCATGTAT CTCATGAAAG 1321 CTGACCTGAT GCCTTTCCTG TATTGGAATA TGATGCTAAG GGGTTACTGG GGAGGACCAG 1381 CGTTTCTGCG CAAGTTGTTT CATCTTGGTA TGAGTTCCCC AACTTTCTTG TACAAAGTGG 1441 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1501 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1561 ATGATTCCTA TGGTCTCCAG TCTACACGCG TTAAGTCgac aatcaacctc tggattacaa 1621 aatttgtgaa agatt