Construct: ORF TRCN0000478196
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011220.1_s317c1
- Derived from:
- ccsbBroadEn_10834
- DNA Barcode:
- AGCATTCCAACAGCCTCCTAAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GABRR1 (2569)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478196
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2569 | GABRR1 | gamma-aminobutyric acid typ... | NM_002042.5 | 98.4% | 98.5% | (many diffs) |
| 2 | human | 2569 | GABRR1 | gamma-aminobutyric acid typ... | NM_001256703.1 | 94.8% | 95% | (many diffs) |
| 3 | human | 2569 | GABRR1 | gamma-aminobutyric acid typ... | NM_001256704.1 | 82.5% | 82.7% | 0_1ins246;177C>T;924G>A |
| 4 | human | 2569 | GABRR1 | gamma-aminobutyric acid typ... | NM_001267582.1 | 82.5% | 82.7% | 0_1ins246;177C>T;924G>A |
| 5 | human | 2569 | GABRR1 | gamma-aminobutyric acid typ... | XM_017010689.1 | 82.5% | 82.7% | 0_1ins246;177C>T;924G>A |
| 6 | mouse | 14408 | Gabrr1 | gamma-aminobutyric acid (GA... | NM_008075.2 | 86.5% | 93.1% | (many diffs) |
| 7 | mouse | 14408 | Gabrr1 | gamma-aminobutyric acid (GA... | XM_006537613.3 | 80.8% | 82.9% | (many diffs) |
| 8 | mouse | 14408 | Gabrr1 | gamma-aminobutyric acid (GA... | XM_006537612.1 | 80.4% | 82% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1491
- ORF length:
- 1422
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagatttggc atctttcttt tgtggtgggg atgggttttg gccactgaaa 121 gcagaatgca ctggcccgga agagaagtcc acgagatgtc taagaaaggc agtaggcccc 181 aaagacaaag acgagaagta catgaagatg cccacaagca agtcagccca attctgagac 241 gaagtcctga catcaccaaa tcgcctctga caaagtcaga acagcttctg aggatagatg 301 accatgattt cagcatgagg cctggctttg gaggccctgc cattcctgtt ggtgtggatg 361 tgcaggtgga gagtttggat agcatctcag aggttgacat ggactttacg atgaccctct 421 acctgaggca ctactggaag gacgagaggc tgtcttttcc aagcaccaac aacctcagca 481 tgacgtttga tggccggctg gtcaagaaga tctgggtccc tgacatgttt ttcgtgcact 541 ccaaacgctc cttcatccac gacaccacca cagacaacgt catgttgcgg gtccagcctg 601 atgggaaagt gctctatagt ctcagggtta cagtaactgc aatgtgcaac atggacttca 661 gccgatttcc cttggacaca caaacgtgct ctcttgaaat tgaaagctat gcctatacag 721 aagatgacct catgctgtac tggaaaaagg gcaatgactc cttaaagaca gatgaacgga 781 tctcactctc ccagttcctc attcaggaat tccacaccac caccaaactg gctttctaca 841 gcagcacagg ctggtacaac cgtctctaca ttaatttcac gttgcgtcgc cacatcttct 901 tcttcttgct ccaaacttat ttccccgcta ccctgatggt catgctgtcc tgggtgtcct 961 tctggatcga ccgcagagcc gtgcctgcca gagtcccctt aggtatcaca acggtgctga 1021 ccatgtccac catcatcacg ggcgtgaatg cctccatgcc gcgcgtctcc tacatcaagg 1081 ccgtggacat ctacctctgg gtcagctttg tgttcgtgtt cctctcggtg ctggagtatg 1141 cggccgtcaa ctacctgacc actgtgcagg agaggaagga acagaagctg cgggagaagc 1201 ttccctgcac cagcggatta ccTCCGCCCC GCACTGCAAT GCTGGACGGC AACTACAGTG 1261 ATGGGGAGGT GAATGACCTG GACAACTACA TGCCAGAGAA TGGAGAGAAG CCCGACAGGA 1321 TGATGGTGCA GCTGACCCTG GCCTCAGAGA GGAGCTCCCC ACAGAGGAAA AGTCAGAGAA 1381 GCAGCTATGT GAGCATGAGA ATCGACACCC ACGCCATTGA TAAATACTCC AGGATCATCT 1441 TTCCAGCAGC ATACATTTTA TTCAATTTAA TATACTGGTC TATTTTCTCC TTGCCAACTT 1501 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1561 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1621 CTTGTGGAAA GGACGAAGCA TTCCAACAGC CTCCTAAAAT ACGCGTTAAG TCgacaatca 1681 acctctggat tacaaaattt gtgaaagatt