Construct: ORF TRCN0000478202
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017699.2_s317c1
- Derived from:
- ccsbBroadEn_15858
- DNA Barcode:
- TCCGAGAGGTATTAACAAATTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLIC5 (53405)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478202
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53405 | CLIC5 | chloride intracellular chan... | XR_926258.3 | 4.3% | 1_3390del;3598_4773del | |
| 2 | human | 53405 | CLIC5 | chloride intracellular chan... | NR_045672.1 | 3.8% | 1_3930del;4138_5330del | |
| 3 | human | 53405 | CLIC5 | chloride intracellular chan... | NR_045673.1 | 3.8% | 1_4004del;4212_5404del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 297
- ORF length:
- 207
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCCCTGTC CTCAGCATTC CCTTCTTGTG 121 CTGGTGGGGC CAGTGAAGTC TTGATCTTAT CAGAAAAAGG CCACACCAAG TGCGAGTTTT 181 CCCAGGCTGA CTTTCCAGGC CCTTATCAAA TGAAACAACA GAAGCTCTTC ACAGTTCTGT 241 GCCCCATGGC CACTCCACAG ACAGACAATA CCAAGCATCT TAGAACTGTC ATAAGATACC 301 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 361 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 421 ATATATCTTG TGGAAAGGAC GATCCGAGAG GTATTAACAA ATTGCAACGC GTTAAGTCga 481 caatcaacct ctggattaca aaatttgtga aagatt