Construct: ORF TRCN0000478213
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016029.1_s317c1
- Derived from:
- ccsbBroadEn_15234
- DNA Barcode:
- GTCCGAAATCGGCCCAATGCGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TARS3 (123283)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478213
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 123283 | TARS3 | threonyl-tRNA synthetase 3 | NM_152334.3 | 99.9% | 99.7% | 32T>N;287C>G |
| 2 | human | 123283 | TARS3 | threonyl-tRNA synthetase 3 | XM_006720386.4 | 88.6% | 85.4% | (many diffs) |
| 3 | human | 123283 | TARS3 | threonyl-tRNA synthetase 3 | XM_017021912.2 | 83.4% | 81.7% | (many diffs) |
| 4 | human | 123283 | TARS3 | threonyl-tRNA synthetase 3 | XR_001751084.2 | 75% | (many diffs) | |
| 5 | human | 123283 | TARS3 | threonyl-tRNA synthetase 3 | XR_931748.3 | 72.7% | (many diffs) | |
| 6 | human | 123283 | TARS3 | threonyl-tRNA synthetase 3 | XM_024449836.1 | 69.7% | 69.7% | 0_1ins729 |
| 7 | human | 123283 | TARS3 | threonyl-tRNA synthetase 3 | XR_931749.3 | 66.4% | (many diffs) | |
| 8 | human | 123283 | TARS3 | threonyl-tRNA synthetase 3 | XR_001751082.2 | 57.8% | (many diffs) | |
| 9 | human | 123283 | TARS3 | threonyl-tRNA synthetase 3 | XR_001751083.2 | 56.1% | (many diffs) | |
| 10 | mouse | 272396 | Tarsl2 | threonyl-tRNA synthetase-li... | NM_172310.2 | 84.3% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2475
- ORF length:
- 2406
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggccgag gccctggcgg cggaggccgn ggcgtcgcgc ctggagcggc 121 aggaggagga catccgctgg ctgtggtcgg aggtcgagcg cctgagggac gagcagctga 181 acgcgcccta cagctgccag gcggaggggc cgtgcctcac gcgggaggtg gcgcagctcc 241 gggccgagaa ctgcgacctg cgccaccgcc tgtgcagcct gcggctgtgc ctcgccgagg 301 agcggagccg ccaggccacg ctggagagcg cggagctaga ggcggcgcag gagggcggcg 361 cacagcctcc tcctagtcaa agccaagaca aggacatgaa aaagaagaaa atgaaggaaa 421 gcgaggctga cagcgaggtg aagcatcaac caattttcat aaaagaaaga ttgaagcttt 481 ttgaaatact gaagaaagac catcagctct tacttgccat ttatggaaaa aagggggata 541 caagcaacat catcacagta agagtggctg atgggcaaac agtgcaaggg gaagtctgga 601 aaacaacgcc ttaccaagtg gctgctgaaa ttagtcagga actggctgaa agcacggtaa 661 tagccaaagt caatggtgaa ctgtgggacc tggaccgccc attggaaggg gactcttctc 721 tagagctgct tacatttgat aatgaggaag ctcaagctgt gtactggcac tccagtgctc 781 acattcttgg ggaggccatg gagctttact atggaggcca cctgtgctac ggtccgccca 841 ttgaaaatgg attttattat gacatgttca ttgaagacag agcagtgtcc agcacagaat 901 tgtcagccct ggagaatata tgtaaagcca tcataaaaga aaagcaacct tttgaaagac 961 tagaagtcag caaggaaatc ctcctggaaa tgtttaagta caataaattt aaatgccgca 1021 ttctgaatga gaaagttaac actgcaacta ccaccgtgta caggtgcggt ccattaattg 1081 acctttgcaa aggtccacat gtaagacaca ctggaaaaat taaaaccatc aaaattttta 1141 agaattcctc aacatattgg gagggcaatc cggaaatgga aacattgcag aggatctatg 1201 gaatatcctt tcctgataac aagatgatga gagactggga aaagttccaa gaggaagcaa 1261 agaaccgaga tcacaggaag atcgggaagg aacaagaact tttctttttc cacgatttga 1321 gtcctggaag ctgttttttc cttcccagag gagccttcat ttataatacg cttacagatt 1381 tcatacgaga ggaatatcac aaacgggact tcacggaggt gctctctccc aatatgtaca 1441 acagtaaact ctgggaagcc tcaggccact ggcagcatta cagcgagaac atgtttacct 1501 ttgagattga aaaggacact tttgccctca aacccatgaa ttgtccaggg cactgtctaa 1561 tgtttgccca tcgtccacga tcttggaggg aaatgcctat tagatttgct gattttggag 1621 ttctgcatag aaatgaactg tcggggactc tcagcggctt gaccagagtg aggcgcttcc 1681 agcaggacga tgctcacatt ttttgcacag tggagcagat tgaagaagaa ataaaggggt 1741 gtttgcagtt tttgcaatct gtttactcaa catttggctt ctcctttcaa ttaaacctgt 1801 caacaaggcc ggaaaacttc ctaggagaga ttgagatgtg gaatgaggct gagaagcaac 1861 tgcagaacag cttgatggac tttggagaac cgtggaaaat gaacccagga gatggagcat 1921 tttatggccc taaaattgac ataaaaatca aggatgctat tggcagatac catcaatgtg 1981 ctacaattca gctggacttc caactgccta ttagatttaa tctcacatat gttagtaagg 2041 atggggatga taagaagaga cctgtgatca ttcatcgagc cattttggga tcagtggaaa 2101 gaatgatagc cattctttca gaaaactatg gcggaaaatg gcctttctgg ctatctcctc 2161 gtcaggtgat ggtcatCCCT GTGGGGCCAA CTTGTGAAAA ATATGCACTT CAGGTATCCA 2221 GTGAATTTTT TGAAGAAGGA TTTATGGCTG ACGTTGACTT GGATCACAGT TGTACACTAA 2281 ATAAGAAAAT ACGAAATGCA CAGCTGGCTC AGTATAATTT TATTTTGGTG GTTGGAGAAA 2341 AGGAAAAGAT AGATAATGCT GTAAACGTGC GAACAAGAGA CAACAAAATT CATGGAGAGA 2401 TTTTAGTAAC TTCTGCCATT GATAAACTGA AGAATCTCAG GAAGACACGG ACACTCAATG 2461 CTGAGGAGGC CTTTTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2521 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2581 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTCCGAAATC GGCCCAATGC 2641 GAAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt