Construct: ORF TRCN0000478213

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016029.1_s317c1
Derived from:
ccsbBroadEn_15234
DNA Barcode:
GTCCGAAATCGGCCCAATGCGAAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TARS3 (123283)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478213
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 123283 TARS3 threonyl-tRNA synthetase 3 NM_152334.3 99.9% 99.7% 32T>N;287C>G
2 human 123283 TARS3 threonyl-tRNA synthetase 3 XM_006720386.4 88.6% 85.4% (many diffs)
3 human 123283 TARS3 threonyl-tRNA synthetase 3 XM_017021912.2 83.4% 81.7% (many diffs)
4 human 123283 TARS3 threonyl-tRNA synthetase 3 XR_001751084.2 75% (many diffs)
5 human 123283 TARS3 threonyl-tRNA synthetase 3 XR_931748.3 72.7% (many diffs)
6 human 123283 TARS3 threonyl-tRNA synthetase 3 XM_024449836.1 69.7% 69.7% 0_1ins729
7 human 123283 TARS3 threonyl-tRNA synthetase 3 XR_931749.3 66.4% (many diffs)
8 human 123283 TARS3 threonyl-tRNA synthetase 3 XR_001751082.2 57.8% (many diffs)
9 human 123283 TARS3 threonyl-tRNA synthetase 3 XR_001751083.2 56.1% (many diffs)
10 mouse 272396 Tarsl2 threonyl-tRNA synthetase-li... NM_172310.2 84.3% 85.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2475
ORF length:
2406
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggccgag gccctggcgg cggaggccgn ggcgtcgcgc ctggagcggc
121aggaggagga catccgctgg ctgtggtcgg aggtcgagcg cctgagggac gagcagctga
181acgcgcccta cagctgccag gcggaggggc cgtgcctcac gcgggaggtg gcgcagctcc
241gggccgagaa ctgcgacctg cgccaccgcc tgtgcagcct gcggctgtgc ctcgccgagg
301agcggagccg ccaggccacg ctggagagcg cggagctaga ggcggcgcag gagggcggcg
361cacagcctcc tcctagtcaa agccaagaca aggacatgaa aaagaagaaa atgaaggaaa
421gcgaggctga cagcgaggtg aagcatcaac caattttcat aaaagaaaga ttgaagcttt
481ttgaaatact gaagaaagac catcagctct tacttgccat ttatggaaaa aagggggata
541caagcaacat catcacagta agagtggctg atgggcaaac agtgcaaggg gaagtctgga
601aaacaacgcc ttaccaagtg gctgctgaaa ttagtcagga actggctgaa agcacggtaa
661tagccaaagt caatggtgaa ctgtgggacc tggaccgccc attggaaggg gactcttctc
721tagagctgct tacatttgat aatgaggaag ctcaagctgt gtactggcac tccagtgctc
781acattcttgg ggaggccatg gagctttact atggaggcca cctgtgctac ggtccgccca
841ttgaaaatgg attttattat gacatgttca ttgaagacag agcagtgtcc agcacagaat
901tgtcagccct ggagaatata tgtaaagcca tcataaaaga aaagcaacct tttgaaagac
961tagaagtcag caaggaaatc ctcctggaaa tgtttaagta caataaattt aaatgccgca
1021ttctgaatga gaaagttaac actgcaacta ccaccgtgta caggtgcggt ccattaattg
1081acctttgcaa aggtccacat gtaagacaca ctggaaaaat taaaaccatc aaaattttta
1141agaattcctc aacatattgg gagggcaatc cggaaatgga aacattgcag aggatctatg
1201gaatatcctt tcctgataac aagatgatga gagactggga aaagttccaa gaggaagcaa
1261agaaccgaga tcacaggaag atcgggaagg aacaagaact tttctttttc cacgatttga
1321gtcctggaag ctgttttttc cttcccagag gagccttcat ttataatacg cttacagatt
1381tcatacgaga ggaatatcac aaacgggact tcacggaggt gctctctccc aatatgtaca
1441acagtaaact ctgggaagcc tcaggccact ggcagcatta cagcgagaac atgtttacct
1501ttgagattga aaaggacact tttgccctca aacccatgaa ttgtccaggg cactgtctaa
1561tgtttgccca tcgtccacga tcttggaggg aaatgcctat tagatttgct gattttggag
1621ttctgcatag aaatgaactg tcggggactc tcagcggctt gaccagagtg aggcgcttcc
1681agcaggacga tgctcacatt ttttgcacag tggagcagat tgaagaagaa ataaaggggt
1741gtttgcagtt tttgcaatct gtttactcaa catttggctt ctcctttcaa ttaaacctgt
1801caacaaggcc ggaaaacttc ctaggagaga ttgagatgtg gaatgaggct gagaagcaac
1861tgcagaacag cttgatggac tttggagaac cgtggaaaat gaacccagga gatggagcat
1921tttatggccc taaaattgac ataaaaatca aggatgctat tggcagatac catcaatgtg
1981ctacaattca gctggacttc caactgccta ttagatttaa tctcacatat gttagtaagg
2041atggggatga taagaagaga cctgtgatca ttcatcgagc cattttggga tcagtggaaa
2101gaatgatagc cattctttca gaaaactatg gcggaaaatg gcctttctgg ctatctcctc
2161gtcaggtgat ggtcatCCCT GTGGGGCCAA CTTGTGAAAA ATATGCACTT CAGGTATCCA
2221GTGAATTTTT TGAAGAAGGA TTTATGGCTG ACGTTGACTT GGATCACAGT TGTACACTAA
2281ATAAGAAAAT ACGAAATGCA CAGCTGGCTC AGTATAATTT TATTTTGGTG GTTGGAGAAA
2341AGGAAAAGAT AGATAATGCT GTAAACGTGC GAACAAGAGA CAACAAAATT CATGGAGAGA
2401TTTTAGTAAC TTCTGCCATT GATAAACTGA AGAATCTCAG GAAGACACGG ACACTCAATG
2461CTGAGGAGGC CTTTTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2521CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
2581TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTCCGAAATC GGCCCAATGC
2641GAAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)