Transcript: Human XM_006720386.4

PREDICTED: Homo sapiens threonyl-tRNA synthetase 3 (TARS3), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TARS3 (123283)
Length:
3024
CDS:
46..2184

Additional Resources:

NCBI RefSeq record:
XM_006720386.4
NBCI Gene record:
TARS3 (123283)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006720386.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000241521 AGCTGACATGCACGAAGTTTA pLKO_005 2527 3UTR 100% 13.200 10.560 N TARS3 n/a
2 TRCN0000180055 CGTGCGAACAAGAGACAACAA pLKO.1 2270 3UTR 100% 4.950 3.960 N TARS3 n/a
3 TRCN0000180799 GCAGAACAGCTTGATGGACTT pLKO.1 1839 CDS 100% 4.050 3.240 N TARS3 n/a
4 TRCN0000241519 GGACTTCCAACTGCCTATTAG pLKO_005 1971 CDS 100% 13.200 9.240 N TARS3 n/a
5 TRCN0000147519 GATACCATCAATGTGCTACAA pLKO.1 1943 CDS 100% 4.950 3.465 N TARS3 n/a
6 TRCN0000241518 TACCATCAATGTGCTACAATT pLKO_005 1945 CDS 100% 0.000 0.000 N TARS3 n/a
7 TRCN0000241520 TTGGAGGGAAATGCCTATTAG pLKO_005 1560 CDS 100% 13.200 7.920 N TARS3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006720386.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15234 pDONR223 94.8% 88.6% 85.4% None (many diffs) n/a
2 ccsbBroad304_15234 pLX_304 0% 88.6% 85.4% V5 (many diffs) n/a
3 TRCN0000478213 GTCCGAAATCGGCCCAATGCGAAA pLX_317 12.7% 88.6% 85.4% V5 (many diffs) n/a
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