Construct: ORF TRCN0000478220
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013450.1_s317c1
- Derived from:
- ccsbBroadEn_13954
- DNA Barcode:
- ACCCCAAGGGTATTCATAAGTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC4A2 (6522)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478220
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6522 | SLC4A2 | solute carrier family 4 mem... | NM_001199694.2 | 93.6% | 92.7% | (many diffs) |
| 2 | human | 6522 | SLC4A2 | solute carrier family 4 mem... | NM_001199692.2 | 93.3% | 93.3% | 58_303del;1071T>C;2202G>A |
| 3 | human | 6522 | SLC4A2 | solute carrier family 4 mem... | NM_003040.4 | 93.3% | 93.3% | 58_303del;1071T>C;2202G>A |
| 4 | human | 6522 | SLC4A2 | solute carrier family 4 mem... | NM_001199693.1 | 93.2% | 92.3% | (many diffs) |
| 5 | human | 6522 | SLC4A2 | solute carrier family 4 mem... | XM_006716094.3 | 47% | 46.4% | (many diffs) |
| 6 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | XM_006535656.3 | 81.6% | 87.3% | (many diffs) |
| 7 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | NM_001253892.1 | 81.5% | 87.7% | (many diffs) |
| 8 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | NM_009207.3 | 81.5% | 87.7% | (many diffs) |
| 9 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | XM_006535650.1 | 81.5% | 87.7% | (many diffs) |
| 10 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | XM_006535651.3 | 81.5% | 87.7% | (many diffs) |
| 11 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | XM_006535655.3 | 81.3% | 86.9% | (many diffs) |
| 12 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | XM_006535653.1 | 81.2% | 87.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3543
- ORF length:
- 3477
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cagcgcccct cggcgccccg ccaagggcgc agattctttc tgtacgccag 121 agccaggacg gaagcctcga aggcgcccgg gagcctcccc gactggagaa accccgacca 181 ttgaggaggg ggaggaagat gaggatgagg ccagcgaggc tgagggggcc cgggctctca 241 ctcagccgtc ccctgtctcc acaccctcct cggtgcagtt ctttctccaa gaggatgaca 301 gtgctgaccg gaaggcagag aggaccagtc catcttcccc tgcaccactg ccccaccagg 361 aggcgactcc tcgggcctcc aaaggggccc aggctggaac ccaggtggag gaggcggagg 421 cggaggcggt ggcggtggcc agtggcactg cagggggtga cgacgggggt gcctcggggc 481 gccccctgcc caaagcccag cctgggcacc gcagctacaa ccttcaggag aggaggcgca 541 tcgggagcat gactggggct gagcaggcac tgctgccccg ggtccccacg gatgagattg 601 aggcccagac gctggccacg gccgacctag acctcatgaa gagtcaccgg tttgaggacg 661 ttcctggggt gcggcggcac ttggtgcgga agaatgccaa aggttccaca cagagtggcc 721 gagaagggcg ggagcctggc cccacacctc gggcccgacc ccgggccccc cacaagcccc 781 atgaggtgtt tgtggagctg aatgagttgc tcctggacaa aaaccaggag ccccagtggc 841 gggagacagc tcgctggatc aaatttgaag aggacgtgga ggaggagacc gagcgctggg 901 ggaagcccca cgtggcctcc ctctccttcc gcagtctcct ggagctccgc aggaccctgg 961 cccatggggc tgtgctcttg gatctggacc agcagaccct gcccggagtg gcccaccagg 1021 tggtggagca gatggtcatc tctgaccaga tcaaggccga ggacagggcc aacgtgctgc 1081 gggctctgct gttgaaacac agccacccaa gtgatgagaa ggacttctcc ttcccccgca 1141 acatctcagc tggctccctg ggctccctgc tggggcatca ccatggtcag ggggctgaga 1201 gtgaccccca cgtcaccgag cctctcatgg gaggtgttcc tgagacccgg ctggaggtgg 1261 agcgagagcg tgagctgccg cctccagcac caccagctgg catcacccgc tccaagtcca 1321 agcacgagct gaaactgctg gagaagattc ctgagaatgc cgaggccacg gtggtccttg 1381 tgggctgcgt ggagttcctc tcccgcccca ccatggcctt tgtgcggctc cgggaggctg 1441 tggagttgga cgcagtgttg gaggtgccgg tgcctgtgcg tttcctcttc ctgctgctgg 1501 gcccgagtag tgccaacatg gactaccacg agatcggccg ctccatctcc accctcatgt 1561 cagacaagca attccacgag gcagcctacc tggctgacga gcgggaggac ctgctgacgg 1621 ccatcaacgc cttcctggac tgcagcgtgg tgctgccgcc ttcagaagtg cagggcgagg 1681 agctgctgcg ctctgtggcc cacttccagc gccagatgct caagaagcga gaggagcagg 1741 gccggctgct acctacaggg gctgggctgg agcccaaatc tgcccaagat aaggcgctcc 1801 tgcagatggt agaggcggca ggggcagctg aagatgatcc ccttcggcgg acggggcggc 1861 cctttggggg gctgatccga gatgtgcggc gccgctatcc ccactacctg agtgacttcc 1921 gagatgcact tgaccctcag tgcctggccg cagtcatctt catctacttt gccgccctgt 1981 ctcctgccat cacctttggg gggctgctgg gagagaagac acaggacctg ataggggtgt 2041 cggagctgat tatgtccaca gcgctccagg gcgtggtctt ctgcctgctg ggtgcccagc 2101 ccctgttggt gatcggcttc tcagggcccc tgctggtctt tgaggaggcc ttcttctcgt 2161 tctgtagcag caaccacctg gagtacctgg tgggccgtgt gtggatcggc ttctggctgg 2221 tgttcctggc cctgctcatg gtggccctgg aggggagctt cctggtccgc ttcgtctccc 2281 gcttcaccca ggagatcttc gccttcttga tctcactcat cttcatctat gagaccttct 2341 acaagctggt gaagatcttc caggagcacc ccctgcatgg ctgctcagcc tccaacagct 2401 cagaggtgga cggcggtgag aacatgacat gggccggggc aagacccacg ctggggccgg 2461 gcaacaggag cttggctggg cagtctgggc aggggaagcc ccggggccag cccaacacgg 2521 ccctgctgtc gctggtgctc atggccggca ccttcttcat cgccttcttc ctgcgcaaat 2581 tcaagaacag ccggttcttt cctggccgga tccggcgggt gattggggac tttggggtgc 2641 ccatcgccat cctcatcatg gtgcttgtgg attacagtat tgaggacacc tatacccaga 2701 agctgagcgt tcccagtgga ttctcggtga ctgccccaga aaagaggggc tgggtcatca 2761 accccctggg agagaagagc cccttccctg tgtggatgat ggttgccagc ctgctgcccg 2821 ccatcctggt cttcattctc atcttcatgg agacacagat caccacgctc atcatctcca 2881 agaaggagcg catgctgcag aagggctccg gcttccacct ggacctgctg ctcatcgtgg 2941 ccatgggcgg catctgtgcc ctctttggcc tgccctggtt ggctgctgcc actgtccgct 3001 ctgtcactca cgccaacgcg ctcactgtca tgagcaaggc tgtggcacct ggggacaagc 3061 ccaagattca ggaagtcaag gagcagcggg tgacggggct gctggttgcc ctgcttgtgg 3121 gcctctccat agttatcggg gatctgctcc ggcagatccc cctggccgtg ctctttggaa 3181 ttttcctgta catgggagtc acctccctta acgggatcca gttctatgag cggctgcatc 3241 tgctgctcat gccgcccaaa caccacccag atgtcactta cgtcaagaag gtccggaccc 3301 TCCGTATGCA CCTGTTCACG GCCCTGCAGC TGCTCTGCCT GGCCCTGCTC TGGGCCGTCA 3361 TGTCCACAGC TGCCTCCCTG GCCTTCCCCT TCATCCTCAT CCTCACAGTG CCGCTCCGCA 3421 TGGTGGTGCT CACCCGTATC TTCACCGACC GAGAGATGAA ATGTCTGGAT GCTAACGAGG 3481 CAGAGCCGGT GTTTGATGAG CGGGAGGGTG TGGACGAGTA CAATGAGATG CCCATGCCTG 3541 TGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 3601 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 3661 GGCTTTATAT ATCTTGTGGA AAGGACGAAC CCCAAGGGTA TTCATAAGTG TCACGCGTTA 3721 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt