Construct: ORF TRCN0000478232
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015917.1_s317c1
- Derived from:
- ccsbBroadEn_01452
- DNA Barcode:
- AGTCCCCACTGTAGGACGATGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS13 (6207)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478232
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6207 | RPS13 | ribosomal protein S13 | NM_001017.3 | 100% | 100% | |
| 2 | mouse | 68052 | Rps13 | ribosomal protein S13 | NM_026533.3 | 86.9% | 100% | (many diffs) |
| 3 | mouse | 628061 | Rps13-ps4 | ribosomal protein S13, pseu... | XR_879249.2 | 63% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 519
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tcgcatgcat gctcccggga agggcctgtc ccagtcggct ttaccctatc 121 gacgcagcgt ccccacttgg ttgaagttga catctgacga cgtgaaggag cagatttaca 181 aactggccaa gaagggcctt actccttcac agatcggtgt aatccTGAGA GATTCACATG 241 GTGTTGCACA AGTACGTTTT GTGACAGGCA ATAAAATTTT AAGAATTCTT AAGTCTAAGG 301 GACTTGCTCC TGATCTTCCT GAAGATCTCT ACCATTTAAT TAAGAAAGCA GTTGCTGTTC 361 GAAAGCATCT TGAGAGGAAC AGAAAGGATA AGGATGCTAA ATTCCGTCTG ATTCTAATAG 421 AGAGCCGGAT TCACCGTTTG GCTCGATATT ATAAGACCAA GCGAGTCCTC CCTCCCAATT 481 GGAAATATGA ATCATCTACA GCCTCTGCCC TGGTCGCATA CCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGAAGTCCC CACTGTAGGA CGATGTACAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt