Construct: ORF TRCN0000478234
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004503.1_s317c1
- Derived from:
- ccsbBroadEn_10938
- DNA Barcode:
- TATAAATTTGACGCGGACTCTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IPO5 (3843)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478234
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3843 | IPO5 | importin 5 | NM_002271.6 | 100% | 100% | |
| 2 | human | 3843 | IPO5 | importin 5 | XM_005254052.3 | 98.3% | 98.3% | 1_54del |
| 3 | human | 3843 | IPO5 | importin 5 | XM_005254053.3 | 98.3% | 98.3% | 1_54del |
| 4 | human | 3843 | IPO5 | importin 5 | XM_011521087.2 | 98.3% | 98.3% | 1_54del |
| 5 | human | 3843 | IPO5 | importin 5 | XM_011521088.2 | 98.3% | 98.3% | 1_54del |
| 6 | human | 3843 | IPO5 | importin 5 | XM_011521089.2 | 98.3% | 98.3% | 1_54del |
| 7 | human | 3843 | IPO5 | importin 5 | XM_011521090.2 | 98.3% | 98.3% | 1_54del |
| 8 | human | 3843 | IPO5 | importin 5 | XM_017020562.1 | 98.3% | 98.3% | 1_54del |
| 9 | human | 3843 | IPO5 | importin 5 | XM_024449346.1 | 98.3% | 98.3% | 1_54del |
| 10 | mouse | 70572 | Ipo5 | importin 5 | NM_023579.4 | 89.7% | 98.2% | (many diffs) |
| 11 | mouse | 70572 | Ipo5 | importin 5 | XM_030248018.1 | 89.7% | 98.2% | (many diffs) |
| 12 | mouse | 70572 | Ipo5 | importin 5 | XM_030248019.1 | 89.7% | 98.2% | (many diffs) |
| 13 | mouse | 70572 | Ipo5 | importin 5 | NM_001360602.1 | 84.5% | 92.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3357
- ORF length:
- 3291
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggccgcg gcggagcagc aacagttcta cctgctcctg ggaaacctgc 121 tcagccccga caatgtggtc cggaaacagg cagaggaaac ctatgagaat atcccaggcc 181 agtcaaagat cacattcctc ttacaagcca tcagaaatac aacagctgct gaagaggcta 241 gacaaatggc cgccgttctc ctaagacgtc tcttgtcctc tgcatttgat gaagtctatc 301 cagcacttcc ctctgatgtt cagactgcca tcaagagtga gctactcatg attattcaga 361 tggaaacaca atctagcatg aggaaaaaag tttgtgatat tgcggcagaa ctggccagga 421 atttaataga tgaggatggc aataaccagt ggcccgaagg tttgaagttc ctttttgatt 481 cagtcagctc tcaaaatgtg ggactgcggg aagctgccct tcacattttc tggaactttc 541 ctggaatttt tgggaaccag caacaacact atttagatgt catcaaacga atgttagttc 601 agtgtatgca agatcaggaa cacccgtcga tcaggacgtt atctgctaga gctacagctg 661 catttatact tgcaaatgag cataatgttg ctctgttcaa acattttgca gacttgctac 721 cgggattcct acaggcggta aatgactcgt gctaccagaa tgatgattct gtcctaaaat 781 ccctcgttga gattgcagat actgttccaa agtatttgcg tcctcacttg gaagcaactc 841 tacagctaag tctaaagttg tgtggagaca ctagcctcaa caatatgcaa cgccagcttg 901 cccttgaagt gatcgtcacc ctctctgaga ctgcagctgc tatgttaaga aaacatacca 961 atattgttgc acagactatt cctcagatgt tagcaatgat ggttgatttg gaagaagatg 1021 aggactgggc aaatgcagat gaactagaag atgatgattt tgacagcaat gcagttgcag 1081 gcgagagtgc tctagatcga atggcttgcg gacttggtgg aaagctcgtt ctgccgatga 1141 tcaaggaaca cattatgcaa atgcttcaaa atcctgactg gaaataccgg catgcaggat 1201 tgatggcctt atctgccatt ggtgaagggt gccaccagca aatggaagga attctaaatg 1261 agatcgtaaa ttttgtttta ctttttctcc aggatcctca tccaagagta aggtatgcag 1321 cctgtaatgc cgtgggacag atggctacag attttgcacc tggtttccaa aagaaatttc 1381 atgagaaggt gattgcagct ctgctgcaga ccatggaaga ccaaggcaat caacgtgtgc 1441 aggcccatgc agctgctgcc ctcattaact ttactgaaga ctgtcccaag tcactactta 1501 ttccatactt ggataatttg gtgaaacatc tgcattccat tatggtactg aagcttcaag 1561 agctgattca gaaaggcacc aagttagttt tggaacaagt tgtgacatcc attgcatcag 1621 ttgccgatac tgcagaagaa aaatttgtcc cctactatga tttatttatg ccatcactga 1681 agcacatcgt tgagaatgcg gttcaaaaag aactgagact tctgagagga aaaactattg 1741 aatgcattag cctcattggt ctggctgttg ggaaggaaaa attcatgcag gatgcatcag 1801 atgtgatgca gcttttgtta aagacccaga cagacttcaa tgatatggaa gatgatgatc 1861 ctcagatctc ttacatgatc tcagcatggg ccagaatgtg caaaatcctt ggaaaagaat 1921 ttcagcaata ccttccagtg gttatggggc ctttaatgaa gacggcttca attaagcccg 1981 aagtagccct tttagatacc caagacatgg agaatatgag tgatgatgat ggttgggaat 2041 ttgtgaacct tggagatcag caaagctttg gtattaaaac tgcaggacta gaagaaaaat 2101 caactgcttg ccagatgttg gtttgctatg ctaaggagtt aaaggaaggc tttgtggagt 2161 acaccgaaca ggttgtcaaa ctgatggtcc ctttactgaa attttatttc cacgatggtg 2221 ttcgagtggc agcagcggaa tccatgcctc ttctcctgga gtgtgcaaga gtccgtggtc 2281 ctgagtatct cacacagatg tggcatttta tgtgtgatgc tctaattaag gccattggta 2341 cagaaccaga ttcagacgtc ctctcagaaa taatgcattc ttttgcaaag tgcattgaag 2401 taatgggaga tggatgcctt aataatgaac actttgaaga actgggaggt atattgaaag 2461 caaagcttga agaacatttt aaaaatcaag aattacgaca agttaaaaga caagatgaag 2521 actatgatga acaggtcgaa gagtcactac aagatgagga tgataatgat gtttatattc 2581 tgaccaaagt gtcagatatt ttacactcaa tattcagtag ctacaaagaa aaggtgttac 2641 catggtttga acagctgctt ccattaattg tcaacctcat ttgtccacat agaccatggc 2701 cagacagaca atggggatta tgcatctttg atgatgtcat agaacactgt agtccagcct 2761 catttaaata cgcagaatat ttcttaagac caatgctcca atatgtatgt gacaacagcc 2821 cagaagtcag gcaagcagct gcatatggcc tgggagtcat ggcacagtac ggtggagata 2881 attatcgccc tttttgtaca gaagcacttc ccctgctggt aagagttatt cagtctgcgg 2941 attctaagac caaagaaaat gtcaatgcta cagagaactg catctcagca gtagggaaaa 3001 tcatgaagtt caagcctgac tgtgtaaacg ttgaagaggt ccTTCCACAC TGGTTGTCTT 3061 GGCTTCCACT ACATGAAGAT AAAGAAGAAG CTGTTCAGAC TTTCAATTAT CTGTGTGACC 3121 TGATTGAAAG TAATCATCCA ATTGTTCTTG GCCCAAACAA TACCAATCTG CCCAAAATAT 3181 TTAGTATAAT TGCGGAAGGA GAAATGCACG AGGCAATTAA ACATGAAGAT CCTTGTGCCA 3241 AACGTCTGGC CAATGTCGTT CGCCAAGTAC AGACTTCTGG AGGACTGTGG ACTGAGTGCA 3301 TAGCACAGCT CAGTCCTGAG CAGCAGGCCG CCATTCAGGA GCTCCTGAAC TCTGCGTGCC 3361 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 3421 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 3481 ATATATCTTG TGGAAAGGAC GATATAAATT TGACGCGGAC TCTGGCACGC GTTAAGTCga 3541 caatcaacct ctggattaca aaatttgtga aagatt