Construct: ORF TRCN0000478234

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004503.1_s317c1
Derived from:
ccsbBroadEn_10938
DNA Barcode:
TATAAATTTGACGCGGACTCTGGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IPO5 (3843)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478234
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3843 IPO5 importin 5 NM_002271.6 100% 100%
2 human 3843 IPO5 importin 5 XM_005254052.3 98.3% 98.3% 1_54del
3 human 3843 IPO5 importin 5 XM_005254053.3 98.3% 98.3% 1_54del
4 human 3843 IPO5 importin 5 XM_011521087.2 98.3% 98.3% 1_54del
5 human 3843 IPO5 importin 5 XM_011521088.2 98.3% 98.3% 1_54del
6 human 3843 IPO5 importin 5 XM_011521089.2 98.3% 98.3% 1_54del
7 human 3843 IPO5 importin 5 XM_011521090.2 98.3% 98.3% 1_54del
8 human 3843 IPO5 importin 5 XM_017020562.1 98.3% 98.3% 1_54del
9 human 3843 IPO5 importin 5 XM_024449346.1 98.3% 98.3% 1_54del
10 mouse 70572 Ipo5 importin 5 NM_023579.4 89.7% 98.2% (many diffs)
11 mouse 70572 Ipo5 importin 5 XM_030248018.1 89.7% 98.2% (many diffs)
12 mouse 70572 Ipo5 importin 5 XM_030248019.1 89.7% 98.2% (many diffs)
13 mouse 70572 Ipo5 importin 5 NM_001360602.1 84.5% 92.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3357
ORF length:
3291
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggcggccgcg gcggagcagc aacagttcta cctgctcctg ggaaacctgc
121tcagccccga caatgtggtc cggaaacagg cagaggaaac ctatgagaat atcccaggcc
181agtcaaagat cacattcctc ttacaagcca tcagaaatac aacagctgct gaagaggcta
241gacaaatggc cgccgttctc ctaagacgtc tcttgtcctc tgcatttgat gaagtctatc
301cagcacttcc ctctgatgtt cagactgcca tcaagagtga gctactcatg attattcaga
361tggaaacaca atctagcatg aggaaaaaag tttgtgatat tgcggcagaa ctggccagga
421atttaataga tgaggatggc aataaccagt ggcccgaagg tttgaagttc ctttttgatt
481cagtcagctc tcaaaatgtg ggactgcggg aagctgccct tcacattttc tggaactttc
541ctggaatttt tgggaaccag caacaacact atttagatgt catcaaacga atgttagttc
601agtgtatgca agatcaggaa cacccgtcga tcaggacgtt atctgctaga gctacagctg
661catttatact tgcaaatgag cataatgttg ctctgttcaa acattttgca gacttgctac
721cgggattcct acaggcggta aatgactcgt gctaccagaa tgatgattct gtcctaaaat
781ccctcgttga gattgcagat actgttccaa agtatttgcg tcctcacttg gaagcaactc
841tacagctaag tctaaagttg tgtggagaca ctagcctcaa caatatgcaa cgccagcttg
901cccttgaagt gatcgtcacc ctctctgaga ctgcagctgc tatgttaaga aaacatacca
961atattgttgc acagactatt cctcagatgt tagcaatgat ggttgatttg gaagaagatg
1021aggactgggc aaatgcagat gaactagaag atgatgattt tgacagcaat gcagttgcag
1081gcgagagtgc tctagatcga atggcttgcg gacttggtgg aaagctcgtt ctgccgatga
1141tcaaggaaca cattatgcaa atgcttcaaa atcctgactg gaaataccgg catgcaggat
1201tgatggcctt atctgccatt ggtgaagggt gccaccagca aatggaagga attctaaatg
1261agatcgtaaa ttttgtttta ctttttctcc aggatcctca tccaagagta aggtatgcag
1321cctgtaatgc cgtgggacag atggctacag attttgcacc tggtttccaa aagaaatttc
1381atgagaaggt gattgcagct ctgctgcaga ccatggaaga ccaaggcaat caacgtgtgc
1441aggcccatgc agctgctgcc ctcattaact ttactgaaga ctgtcccaag tcactactta
1501ttccatactt ggataatttg gtgaaacatc tgcattccat tatggtactg aagcttcaag
1561agctgattca gaaaggcacc aagttagttt tggaacaagt tgtgacatcc attgcatcag
1621ttgccgatac tgcagaagaa aaatttgtcc cctactatga tttatttatg ccatcactga
1681agcacatcgt tgagaatgcg gttcaaaaag aactgagact tctgagagga aaaactattg
1741aatgcattag cctcattggt ctggctgttg ggaaggaaaa attcatgcag gatgcatcag
1801atgtgatgca gcttttgtta aagacccaga cagacttcaa tgatatggaa gatgatgatc
1861ctcagatctc ttacatgatc tcagcatggg ccagaatgtg caaaatcctt ggaaaagaat
1921ttcagcaata ccttccagtg gttatggggc ctttaatgaa gacggcttca attaagcccg
1981aagtagccct tttagatacc caagacatgg agaatatgag tgatgatgat ggttgggaat
2041ttgtgaacct tggagatcag caaagctttg gtattaaaac tgcaggacta gaagaaaaat
2101caactgcttg ccagatgttg gtttgctatg ctaaggagtt aaaggaaggc tttgtggagt
2161acaccgaaca ggttgtcaaa ctgatggtcc ctttactgaa attttatttc cacgatggtg
2221ttcgagtggc agcagcggaa tccatgcctc ttctcctgga gtgtgcaaga gtccgtggtc
2281ctgagtatct cacacagatg tggcatttta tgtgtgatgc tctaattaag gccattggta
2341cagaaccaga ttcagacgtc ctctcagaaa taatgcattc ttttgcaaag tgcattgaag
2401taatgggaga tggatgcctt aataatgaac actttgaaga actgggaggt atattgaaag
2461caaagcttga agaacatttt aaaaatcaag aattacgaca agttaaaaga caagatgaag
2521actatgatga acaggtcgaa gagtcactac aagatgagga tgataatgat gtttatattc
2581tgaccaaagt gtcagatatt ttacactcaa tattcagtag ctacaaagaa aaggtgttac
2641catggtttga acagctgctt ccattaattg tcaacctcat ttgtccacat agaccatggc
2701cagacagaca atggggatta tgcatctttg atgatgtcat agaacactgt agtccagcct
2761catttaaata cgcagaatat ttcttaagac caatgctcca atatgtatgt gacaacagcc
2821cagaagtcag gcaagcagct gcatatggcc tgggagtcat ggcacagtac ggtggagata
2881attatcgccc tttttgtaca gaagcacttc ccctgctggt aagagttatt cagtctgcgg
2941attctaagac caaagaaaat gtcaatgcta cagagaactg catctcagca gtagggaaaa
3001tcatgaagtt caagcctgac tgtgtaaacg ttgaagaggt ccTTCCACAC TGGTTGTCTT
3061GGCTTCCACT ACATGAAGAT AAAGAAGAAG CTGTTCAGAC TTTCAATTAT CTGTGTGACC
3121TGATTGAAAG TAATCATCCA ATTGTTCTTG GCCCAAACAA TACCAATCTG CCCAAAATAT
3181TTAGTATAAT TGCGGAAGGA GAAATGCACG AGGCAATTAA ACATGAAGAT CCTTGTGCCA
3241AACGTCTGGC CAATGTCGTT CGCCAAGTAC AGACTTCTGG AGGACTGTGG ACTGAGTGCA
3301TAGCACAGCT CAGTCCTGAG CAGCAGGCCG CCATTCAGGA GCTCCTGAAC TCTGCGTGCC
3361CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
3421TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
3481ATATATCTTG TGGAAAGGAC GATATAAATT TGACGCGGAC TCTGGCACGC GTTAAGTCga
3541caatcaacct ctggattaca aaatttgtga aagatt

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