Construct: ORF TRCN0000478241
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008661.1_s317c1
- Derived from:
- ccsbBroadEn_13748
- DNA Barcode:
- ACATAAAACGACATGACCAGGGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POM121C (100101267)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478241
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100289561 | LOC100289561 | uncharacterized LOC100289561 | NM_001242713.3 | 60.1% | 48% | (many diffs) |
| 2 | human | 100289561 | LOC100289561 | uncharacterized LOC100289561 | NM_001347879.2 | 60.1% | 48% | (many diffs) |
| 3 | human | 100289561 | LOC100289561 | uncharacterized LOC100289561 | NM_001347880.2 | 60.1% | 48% | (many diffs) |
| 4 | human | 105375356 | LOC105375356 | uncharacterized LOC105375356 | XR_927684.2 | 20.5% | (many diffs) | |
| 5 | human | 105375356 | LOC105375356 | uncharacterized LOC105375356 | XR_001744951.1 | 13.2% | (many diffs) | |
| 6 | human | 105375356 | LOC105375356 | uncharacterized LOC105375356 | XR_001756281.1 | 13.2% | (many diffs) | |
| 7 | human | 100101267 | POM121C | POM121 transmembrane nucleo... | NR_160304.1 | 12.6% | 1_524del;744_1732del | |
| 8 | human | 100101267 | POM121C | POM121 transmembrane nucleo... | NR_160303.1 | 12.5% | 1_543del;763_1751del | |
| 9 | human | 57786 | RBAK | RB associated KRAB zinc finger | NM_001204456.2 | 8.2% | 7.1% | (many diffs) |
| 10 | human | 57786 | RBAK | RB associated KRAB zinc finger | NM_021163.4 | 8.2% | 7.1% | (many diffs) |
| 11 | human | 105375356 | LOC105375356 | uncharacterized LOC105375356 | XR_001744952.1 | 4.7% | (many diffs) | |
| 12 | human | 105375356 | LOC105375356 | uncharacterized LOC105375356 | XR_001756282.1 | 4.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 312
- ORF length:
- 219
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGAACAC ATTGCAGGGG CCAGTGTCAT 121 TCAAAGATGT GGCTGTGGAT TTCACCCAGG AGGAGTGGCG GCAACTGGAC CCTGATGAGA 181 AGATAACATA CGGGGATGTG ATGTTGGAGA ACTACAGCCA TCTAGTTTCC TTGGCTTATG 241 AGGTGGCAAC ATCTTGTACT TCGGAGATTC TGAAGCCGAG CAACTTGCCC AAGTCCTTCT 301 TCTTTTCCCA TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 361 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 421 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACA TAAAACGACA TGACCAGGGG 481 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t