Construct: ORF TRCN0000478248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003430.1_s317c1
- Derived from:
- ccsbBroadEn_03596
- DNA Barcode:
- CGCGAGTATGCAGGAATCCATCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MACROH2A2 (55506)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478248
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55506 | MACROH2A2 | macroH2A.2 histone | NM_018649.3 | 100% | 100% | |
| 2 | mouse | 404634 | Macroh2a2 | macroH2A.2 histone | NM_207000.2 | 90.3% | 98.3% | (many diffs) |
| 3 | mouse | 404634 | Macroh2a2 | macroH2A.2 histone | XM_006513833.4 | 78.9% | 84.6% | (many diffs) |
| 4 | mouse | 67552 | H2afy3 | H2A histone family, member Y3 | NR_003523.1 | 51.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1182
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gggccggagt gggaagaaga aaatgtccaa gctgtcccgt tcagctaggg 121 caggtgtcat ctttccagtg gggaggctga tgcgttatct gaagaaaggg acgttcaagt 181 accggatcag cgtgggcgcc cctgtctaca tggcggcagt cattgagtac ctggcagcgg 241 aaattctaga attggccggc aatgccgcga gggacaacaa gaaggcccgg atagccccga 301 gacacatctt gctggcagtt gccaatgacg aggagctcaa ccagctgcta aaaggagtga 361 ccatcgccag tggaggcgtc ctgcccagaa ttcaccccga actgctggcc aaaaagcgag 421 ggaccaaagg caagtcggaa acgatcctct ccccaccccc agagaaaaga ggcaggaagg 481 ccacgtcagg caagaagggg gggaagaaat ccaaggctgc caaaccacgg acgtccaaaa 541 agtccaaacc aaaggacagc gataaagaag gaacttcaaa ttccacctct gaagatgggc 601 caggggatgg attcaccatt ctgtcttcta agagccttgt tctgggacag aagctgtcct 661 taacccagag tgacatcagc catattggct ccatgagagt ggagggcatt gtccacccaa 721 ccacagccga aattgacctc aaagaagata taggtaaagc cttggaaaag gctgggggaa 781 aagagttctt ggaaacggta aaggagcttc gcaaatccca aggccctttg gaagtcgccg 841 aagccgccgt cagccaatcc agtggactcg cagccaaatt tgtcatccac tgtcacatcc 901 ctcagtgggg cTCCGACAAA TGTGAAGAAC AGCTTGAAGA GACCATCAAA AACTGCCTGT 961 CAGCGGCGGA GGACAAGAAG CTAAAGTCCG TCGCGTTCCC GCCTTTCCCC AGCGGCAGAA 1021 ACTGCTTTCC CAAACAGACT GCGGCCCAGG TGACCCTCAA AGCCATCTCA GCCCACTTTG 1081 ATGACTCGAG CGCGTCCTCG CTGAAGAACG TGTACTTCCT GCTCTTCGAC AGCGAGAGCA 1141 TCGGCATCTA CGTGCAGGAG ATGGCCAAGC TCGACGCCAA GTACCCAACT TTCTTGTACA 1201 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1261 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1321 AGGACGACGC GAGTATGCAG GAATCCATCC CACGCGTTAA GTCgacaatc aacctctgga 1381 ttacaaaatt tgtgaaagat t