Construct: ORF TRCN0000478249
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014427.1_s317c1
- Derived from:
- ccsbBroadEn_15350
- DNA Barcode:
- CCTCCTGACCTGCCACTAAATTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABCA3 (21)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478249
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 21 | ABCA3 | ATP binding cassette subfam... | NM_001089.3 | 12.2% | 12% | (many diffs) |
| 2 | mouse | 27410 | Abca3 | ATP-binding cassette, sub-f... | NM_001039581.2 | 10.2% | 10.6% | (many diffs) |
| 3 | mouse | 27410 | Abca3 | ATP-binding cassette, sub-f... | NM_013855.3 | 10.2% | 10.6% | (many diffs) |
| 4 | mouse | 27410 | Abca3 | ATP-binding cassette, sub-f... | XM_006524370.2 | 10.2% | 10.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgtgctcagg cagctggcgc tcctcctctg gaagaactac accctgcaga 121 agcggaaggt cctggtgacg gtcctggaac tcttcctgcc attgctgttt tctgggatcc 181 tcatctggct ccgcttgaag attcagtcgg aaaatgtgcc caacgccacc atctacccgg 241 gccagtccat ccaggagctg cctctgttct tcaccttccc tccgccagga gacacctggg 301 agcttgccta catcccttct cacagtgacg ctgccaagac cgtcactgag acagtgcgca 361 gggcacttgt gatcaacatg cgagtgcgcg gctttcccTC CGAGAAGGAC TTTGAGGACT 421 ACATTAGGTA CGACAACTGC TCGTCCAGCG TGCTGGCCGC CGTGGTCTTC GAGCACCCCT 481 TCAACCACAG CAAGGAGCCC CTGCCGCTGG CGGTGAAATA TCACCTACGG TTCAGTTACA 541 CACGGAGAAA TTACATGTGG ACCCAAACAG GCTCCTTTTT CCTGAAAGAG ACAGAAGGCT 601 GGCACACTAC TTCCCTTTTC CCGCTTTTCC CAAACCCAGG ACCAAGGGAA CCTACATCCC 661 CTGATGGCGG AGAACCTGGT GAGAAGCTCG GTTACCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 841 TCCTGACCTG CCACTAAATT GAACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt