Construct: ORF TRCN0000478252
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007689.1_s317c1
- Derived from:
- ccsbBroadEn_04300
- DNA Barcode:
- GCTGCGATGTTCCCGTAGCAGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RSPH3 (83861)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478252
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83861 | RSPH3 | radial spoke head 3 | NM_031924.6 | 100% | 100% | |
| 2 | human | 83861 | RSPH3 | radial spoke head 3 | NM_001346418.1 | 82.8% | 82.8% | 629_630ins288 |
| 3 | human | 83861 | RSPH3 | radial spoke head 3 | XR_001743668.2 | 72.6% | 1_450del;2131_2314del | |
| 4 | human | 83861 | RSPH3 | radial spoke head 3 | XM_017011347.2 | 51.4% | 51.4% | 0_1ins816 |
| 5 | human | 83861 | RSPH3 | radial spoke head 3 | XM_024446566.1 | 51.4% | 51.4% | 0_1ins816 |
| 6 | human | 83861 | RSPH3 | radial spoke head 3 | XR_001743669.2 | 51% | 1_450del;2131_3293del | |
| 7 | human | 83861 | RSPH3 | radial spoke head 3 | NR_144434.1 | 26.9% | 1_211del;1892_6237del | |
| 8 | human | 83861 | RSPH3 | radial spoke head 3 | XR_001743671.2 | 22.2% | 0_1ins202;427_428ins142;1337_5672del | |
| 9 | human | 83861 | RSPH3 | radial spoke head 3 | XR_001743670.2 | 21.5% | 1_450del;1079_1080ins288;1843_6178del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1746
- ORF length:
- 1680
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggtcaagcca gccaaggccg cctccctagc caggaacctc gcaaagcgca 121 ggcgcaccta cctgggtggc gcggctgggc ggagtcaaga gccggaagtc ccttgtgccg 181 cagtccttcc cgggaaacct ggggaccgga actgcccgga gttcccgccc ccagacagaa 241 ccctgggttg ctgggcaacc gatgcggccc ccgcagctgg tctctgtggt gctgggagcg 301 agcccagtat agctcccacc tcctgcgctg gtaacctccc gtcccgcccg cctcccttgt 361 tgtcgcctct cctcgcgtcg cggaatcctt gcccttggca ctacttacat ctctccgggt 421 cccacaacac cttagcgccc acctgcttca aagccaagct ccaccgaaag cgaggcagtc 481 agcccccgga catggcctca gcgctgactg atcgcacctc tcgggccccg agcacctaca 541 cctacaccag ccggccccga gcactgccct gccagcgcag ccgttaccgg gacagcctga 601 cgcagccaga tgaagaacct atgcattatg gaaacataat gtatgacaga agggtaattc 661 gaggtaacac ttatgcactc cagacagggc cactgctcgg acggcctgat tctctagagc 721 tccagagaca acgggaggct aggaagaggg ctcttgccag aaaacaagcc caagagcagc 781 tcagaccaca aacacctgaa cctgtggaag gcagaaagca tgtcgatgtg caaacagaat 841 tataccttga agaaattgct gatcgcataa tagaagttga tatggaatgc caaacagatg 901 catttttgga cagaccacca acaccactct ttattcctgc caaaactggc aaagatgtgg 961 ccacccaaat actagaagga gagctctttg actttgatct tgaagttaaa ccagtgttag 1021 aagttttggt ggggaagaca attgagcagt ctcttctgga agtaatggaa gaagaagagc 1081 tggctaacct gcgggccagt cagcgtgagt atgaagaact acggaatagt gaacgtgctg 1141 aagttcaacg acttgaagag caagagaggc gacaccgaga agaaaaagaa cggcgtaaga 1201 aacagcagtg ggaaataatg cacaagcaca acgagacatc acaaaaaatc gccgcccgag 1261 catttgcaca gcgttacctg gctgaccttc tcccgtctgt ttttggcagc ctcagggata 1321 gtggctactt ttatgatccc attgaaagag atattgagat aggatttctt ccatggctaa 1381 tgaatgaagt tGAAAAAACC ATGGAATATA GCATGGTGGG AAGAACAGTG CTTGACATGT 1441 TGATCCGTGA GGTGGTTGAA AAGAGGCTGT GTATGTATGA GCATGGGGAA GACACACATC 1501 AGTCTCCAGA ACCCGAGGAT GAGCCTGGTG GTCCTGGAGC AATGACAGAG TCACTGGAGG 1561 CCTCTGAATT CCTGGAGCAG AGCATGTCAC AGACACGGGA GCTGCTTTTA GATGGAGGCT 1621 ACCTACAAAG AACAACATAT GACAGAAGGT CATCCCAGGA AAGGAAGTTT ATGGAAGAGA 1681 GAGAACTCTT AGGGCAAGAT GAAGAAACAG CAATGAGGAA GTCCTTAGGG GAGGAAGAAT 1741 TGTCATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1801 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1861 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCTGCGATG TTCCCGTAGC AGGTGACGCG 1921 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt