Construct: ORF TRCN0000478258
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001172.1_s317c1
- Derived from:
- ccsbBroadEn_02175
- DNA Barcode:
- TCCGGAGGTATTCTTATTGCTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGED1 (9500)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478258
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9500 | MAGED1 | MAGE family member D1 | NM_001005332.2 | 100% | 100% | |
| 2 | human | 9500 | MAGED1 | MAGE family member D1 | NM_006986.4 | 100% | 100% | |
| 3 | human | 9500 | MAGED1 | MAGE family member D1 | XM_011530835.2 | 100% | 100% | |
| 4 | human | 9500 | MAGED1 | MAGE family member D1 | XM_017029978.1 | 100% | 100% | |
| 5 | human | 9500 | MAGED1 | MAGE family member D1 | XM_017029979.1 | 100% | 100% | |
| 6 | human | 9500 | MAGED1 | MAGE family member D1 | XM_017029980.1 | 100% | 100% | |
| 7 | human | 9500 | MAGED1 | MAGE family member D1 | NM_001005333.2 | 93.2% | 93.2% | 46_213del |
| 8 | mouse | 94275 | Maged1 | melanoma antigen, family D, 1 | NM_019791.2 | 86.8% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2400
- ORF length:
- 2334
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcagaaaatg gactgtggtg cgggcctcct cggcttccag gctgaggcct 121 ccgtagaaga cagcgccttg cttatgcaga ccttgatgga ggccatccag atctcagagg 181 ctccacctac taaccaggcc accgcagctg ctagtcccca gagttcacag cccccaactg 241 ccaatgagat ggctgacatt caggtttcag cagctgccgc taggcctaag tcagccttta 301 aagtccagaa tgccaccaca aaaggcccaa atggtgtcta tgatttctct caggctcata 361 atgccaagga tgtgcccaac acgcagccca aggcagcctt taagtcccaa aatgctaccc 421 caaagggtcc aaatgctgcc tatgattttt cccaggcagc aaccactggt gagttagctg 481 ctaacaagtc tgagatggcc ttcaaggccc agaatgccac tactaaagtg ggcccaaatg 541 ccacctacaa tttctctcag tctctcaatg ccaatgacct ggccaacagc aggcctaaga 601 cccctttcaa ggcttggaat gataccacta aggccccaac agctgatacc cagacccaga 661 atgtaaatca ggccaaaatg gccacttccc aggctgacat agagaccgac ccaggtatct 721 ctgaacctga cggtgcaact gcacagacat cagcagatgg ttcccaggct cagaatctgg 781 agtcccggac aataattcgg ggcaagagga cccgcaagat taataacttg aatgttgaag 841 agaacagcag tggggatcag aggcgggccc cactggctgc agggacctgg aggtctgcac 901 cagttccagt gaccactcag aacccacctg gcgcaccccc caatgtgctc tggcagacgc 961 cattggcttg gcagaacccc tcaggctggc aaaaccagac agccaggcag accccaccag 1021 cacgtcagag ccctccagct aggcagaccc caccagcctg gcagaaccca gtcgcttggc 1081 agaacccagt gatttggcca aacccagtaa tctggcagaa cccagtgatc tggccaaacc 1141 ccattgtctg gcccggccct gttgtctggc cgaatccact ggcctggcag aatccacctg 1201 gatggcagac tccacctgga tggcagaccc caccgggctg gcagggtcct ccagactggc 1261 aaggtcctcc tgactggccg ctaccacccg actggccact gccacctgat tggccacttc 1321 ccactgactg gccactacca cctgactgga tccccgctga ttggccaatt ccacctgact 1381 ggcagaacct gcgcccctcg cctaacctgc gcccttctcc caactcgcgt gcctcacaga 1441 acccaggtgc tgcacagccc cgagatgtgg cccttcttca ggaaagagca aataagttgg 1501 tcaagtactt gatgcttaag gactacacaa aggtgcccat caagcgctca gaaatgctga 1561 gagatatcat ccgtgaatac actgatgttt atccagaaat cattgaacgt gcatgctttg 1621 tcctagagaa gaaatttggg attcaactga aagaaattga caaagaagaa cacctgtata 1681 ttctcatcag tacccccgag tccctggctg gcatactggg aacgaccaaa gacacaccca 1741 agctcggtct cctcttggtg attctgggtg tcatcttcat gaatggcaac cgtgccagtg 1801 aggctgtcct ctgggaggca ctacgcaaga tgggactgcg tcctggggtg agacatcccc 1861 tccttggaga tctaaggaaa cttctcacct atgagtttgt aaagcagaaa tacctggact 1921 acagacgagt gcccaacagc aaccccccgg agtatgagtt cctctggggc ctccgttcct 1981 accatgagac tagcaagatg aaagtgctga gattcattgc agaggttcag aaaagagacc 2041 cTCGTGACTG GACTGCACAG TTCATGGAGG CTGCAGATGA GGCCTTGGAT GCTCTGGATG 2101 CTGCTGCAGC TGAGGCCGAA GCCCGGGCTG AAGCAAGAAC CCGCATGGGA ATTGGAGATG 2161 AGGCTGTGTC TGGGCCCTGG AGCTGGGATG ACATTGAGTT TGAGCTGCTG ACCTGGGATG 2221 AGGAAGGAGA TTTTGGAGAT CCCTGGTCCA GAATTCCATT TACCTTCTGG GCCAGATACC 2281 ACCAGAATGC CCGCTCCAGA TTCCCTCAGA CCTTTGCCGG TCCCATTATT GGTCCTGGTG 2341 GTACAGCCAG TGCCAACTTC GCTGCCAACT TTGGTGCCAT TGGTTTCTTC TGGGTTGAGT 2401 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2461 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2521 TTTATATATC TTGTGGAAAG GACGATCCGG AGGTATTCTT ATTGCTCAAA CGCGTTAAGT 2581 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt