Construct: ORF TRCN0000478273
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004644.1_s317c1
- Derived from:
- ccsbBroadEn_02153
- DNA Barcode:
- AGACGTACTTTTCTTAATCCTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZFYVE9 (9372)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478273
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9372 | ZFYVE9 | zinc finger FYVE-type conta... | NM_004799.3 | 100% | 100% | |
| 2 | human | 9372 | ZFYVE9 | zinc finger FYVE-type conta... | XM_011542437.2 | 100% | 100% | |
| 3 | human | 9372 | ZFYVE9 | zinc finger FYVE-type conta... | NM_007324.4 | 95.8% | 95.8% | 2277_2278ins177 |
| 4 | human | 9372 | ZFYVE9 | zinc finger FYVE-type conta... | XM_017002845.1 | 81.4% | 80.7% | (many diffs) |
| 5 | mouse | 230597 | Zfyve9 | zinc finger, FYVE domain co... | NM_001347163.1 | 87.3% | 87.5% | (many diffs) |
| 6 | mouse | 230597 | Zfyve9 | zinc finger, FYVE domain co... | XM_006502987.3 | 87.3% | 87.5% | (many diffs) |
| 7 | mouse | 230597 | Zfyve9 | zinc finger, FYVE domain co... | XM_006502986.3 | 84.8% | 83.6% | (many diffs) |
| 8 | mouse | 230597 | Zfyve9 | zinc finger, FYVE domain co... | XM_006502989.3 | 83.2% | 83.4% | (many diffs) |
| 9 | mouse | 230597 | Zfyve9 | zinc finger, FYVE domain co... | XM_006502988.3 | 80.9% | 79.6% | (many diffs) |
| 10 | mouse | 230597 | Zfyve9 | zinc finger, FYVE domain co... | NM_183300.2 | 45.5% | 48% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4341
- ORF length:
- 4275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaattacttc caagcagaag cttacaacct ggacaaggtg ttagatgaat 121 ttgaacaaaa cgaagatgaa acagtttctt ctactttatt ggatacaaag tggaataaga 181 ttctagatcc cccttctcac cggctgtcat ttaaccctac tttggccagt gtgaatgaat 241 ctgcagtttc taatgagtca caaccacaac tgaaagtctt ctccctggct cattcagctc 301 ccctgaccac agaggaagag gatcactgtg ctaatggaca ggactgtaat ctaaatccag 361 agattgccac aatgtggatt gatgaaaatg ctgttgcaga agaccagtta attaagagaa 421 actatagttg ggatgatcaa tgcagtgctg ttgaagtggg agagaagaaa tgtggaaacc 481 tggcttgtct gccagatgag aagaatgttc ttgttgtagc cgtcatgcat aactgtgata 541 aaaggacatt acaaaacgat ttacaggatt gtaataatta taatagtcaa tcccttatgg 601 atgcttttag ctgttcactg gataatgaaa acagacaaac tgatcaattt agttttagta 661 taaatgagtc cactgaaaaa gatatgaatt cagagaaaca aatggatcca ttgaatagac 721 cgaaaacaga ggggagatct gttaaccatc tgtgtcctac ttcatctgat agtctagcca 781 gtgtctgttc cccttcacaa ttaaaggatg acggaagtat aggtagagac ccctccatgt 841 ctgcgattac aagtttaacg gttgattcag taatctcatc ccagggaaca gatggatgtc 901 ctgctgttaa aaagcaagag aactatatac cagatgagga cctcactggc aaaatcagct 961 ctcctaggac agatctaggg agtccaaatt ccttttccca catgagtgag gggattttga 1021 tgaaaaaaga gccagcagag gagagcacca ctgaagaatc cctccggtct ggtttacctt 1081 tgcttctcaa accagacatg cctaatgggt ctggaaggaa taatgactgt gaacggtgtt 1141 cagattgcct tgtgcctaat gaagttaggg ctgatgaaaa tgaaggttat gaacatgaag 1201 aaactcttgg cactacagaa ttccttaata tgacagagca tttctctgaa tctcaggaca 1261 tgactaattg gaagttgact aaactaaatg agatgaatga tagccaagta aacgaagaaa 1321 aggaaaagtt tctacagatt agtcagcctg aggacactaa tggtgatagt ggaggacagt 1381 gtgttggatt ggcagatgca ggtctagatt taaaaggaac ttgcattagt gaaagtgaag 1441 aatgtgattt ctccactgtt atagacacac cagcagcaaa ttatctatct aatggttgtg 1501 attcctatgg aatgcaagac ccaggtgttt cttttgttcc aaagacttta ccctccaaag 1561 aagattcagt aacagaagaa aaagaaatag aggaaagcaa gtcagaatgc tactcaaata 1621 tttatgaaca gagaggaaat gaggccacag aagggagtgg actactttta aacagcactg 1681 gtgacctaat gaagaaaaat tatttacata atttctgtag tcaagttcca tcagtgcttg 1741 ggcaatcttc ccccaaggta gtagcaagcc tgccatctat cagtgttcct tttggtggtg 1801 caagacccaa gcaaccttct aatcttaaac ttcaaattcc aaagccatta tcagaccatt 1861 tacaaaatga ctttcctgca aacagtggaa ataatactaa aaataaaaat gatattcttg 1921 ggaaagcaaa attaggggaa aactcagcaa ccaatgtatg cagtccatct ttgggaaaca 1981 tctctaatgt cgatacaaat ggggaacatt tagaaagtta tgaggctgag atctccacta 2041 gaccatgcct tgcattagct ccagatagcc cagataatga tctcagagct ggtcagtttg 2101 gaatttctgc cagaaagcca ttcaccactc tgggtgaggt ggctccagta tgggtaccgg 2161 attctcaggc tccaaattgc atgaaatgtg aagccaggtt tacattcacc aaaaggaggc 2221 atcactgcag agcatgtggg aaggttttct gtgcttcctg ctgtagcctg aaatgtaaac 2281 tgttatacat ggacagaaag gaagctagag tgtgtgtaat ctgccattca gtgctaatga 2341 atgctcaagc ctgggagaac atgatgagtg cctcaagcca gagccctaac cctaacaatc 2401 ctgctgaata ctgttctact atccctccct tgcagcaagc tcaggcctca ggagctctga 2461 gctctccacc tcccactgtg atggtacctg tgggagtttt aaagcaccct ggagcagaag 2521 tggctcagcc cagagagcag aggcgagttt ggtttgctga tgggatcttg cccaatggag 2581 aagttgctga tgcagccaaa ttaacaatga atggaacttc ctctgcagga accctggctg 2641 tgtcacacga cccagtcaag ccagtaacta ccagtcctct accagcagag acggatattt 2701 gtctattctc tgggagtata actcaggttg gaagtcctgt tggaagtgca atgaatctta 2761 ttcctgaaga tggccttcct cccattctca tctccactgg tgtaaaagga gactatgctg 2821 tggaagagaa accatcacag atttcagtaa tgcagcagtt ggaggatggt ggccctgacc 2881 cacttgtatt tgttttaaat gcaaatttgt tgtcaatggt taaaattgta aattatgtga 2941 acaggaagtg ctggtgtttc acaaccaagg gaatgcatgc agtgggtcag tctgagatag 3001 tcattcttct acagtgttta ccggatgaaa agtgtttgcc aaaggatatc tttaatcact 3061 ttgtgcagct ttatcgggat gctctggcag ggaatgtggt gagcaacttg ggacattcct 3121 tcttcagtca aagtttcctt ggcagtaaag aacatggtgg attcttatat gtgacatcta 3181 cctaccagtc actgcaagac ctagtactcc caaccccacc ttacttgttt gggattctta 3241 tccagaaatg ggaaactcct tgggctaaag tatttcctat ccgtctgatg ttgagacttg 3301 gagctgaata tcgactttat ccatgcccac tattcagtgt cagatttcgg aagccattgt 3361 ttggagagac ggggcatacc atcatgaatc ttcttgcaga cttcagaaat taccagtata 3421 ccttgccagt agttcaaggt ttggtggttg atatggaagt tcggaaaact agcatcaaaa 3481 ttcccagcaa cagatacaat gagatgatga aagccatgaa caagtccaat gagcatgtcc 3541 tggcaggagg tgcctgcttc aatgaaaagg cagactctca tcttgtgtgt gtacagaatg 3601 atgatggaaa ctatcagacc caggctatca gtattcacaa tcagcccaga aaagtgactg 3661 gtgccagttt ctttgtgttc agtggcgctc tgaaatcctc ttctggatac cttgccaagt 3721 ccagtattgt ggaagatggt gttatggtcc agattactgc agagaacatg gattccttga 3781 ggcaggcact gcgagagatg aaggacttca ccatcacctg tgggaaggcg gacgcggagg 3841 aaccccagga gcacatccac atccagtggg tggatgatga caagaacgtt agcaagggtg 3901 tcgtaagtcc tatagatggg aagtccatgg agactataac aaatgtgaag atattccatg 3961 gatcagaata taaagcaaat ggaaaagtaa tcagatggac agaggtgttt ttcctagaaa 4021 acgatgacca gcacaattgc cTCAGTGATC CTGCAGATCA CAGTAGATTG ACTGAGCATG 4081 TTGCCAAAGC TTTTTGCCTT GCTCTCTGTC CTCACCTGAA ACTTCTGAAG GAAGATGGAA 4141 TGACCAAACT GGGACTACGT GTGACACTTG ACTCAGATCA GGTTGGCTAT CAAGCAGGGA 4201 GCAATGGCCA GCCCCTTCCC TCGCAGTACA TGAATGATCT GGACAGCGCC TTGGTGCCGG 4261 TGATCCATGG AGGGGCCTGC CAGCTTAGTG AGGGCCCCGT TGTCATGGAA CTCATCTTTT 4321 ATATTCTGGA AAACATCGTA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 4381 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 4441 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGAC GTACTTTTCT 4501 TAATCCTTAG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt