Construct: ORF TRCN0000478274
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012728.1_s317c1
- Derived from:
- ccsbBroadEn_11809
- DNA Barcode:
- CACCATAGGAAAGCATTCACTAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PTPN20 (26095)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478274
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352535.2 | 83.7% | 69.8% | (many diffs) |
| 2 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352531.1 | 79.1% | 68.8% | (many diffs) |
| 3 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XM_017016055.1 | 78.6% | 76.7% | (many diffs) |
| 4 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001320684.1 | 75.6% | 73.7% | (many diffs) |
| 5 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XM_017016053.1 | 75.6% | 73.7% | (many diffs) |
| 6 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001042362.3 | 73% | 62.3% | (many diffs) |
| 7 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352524.1 | 70.6% | 68.4% | (many diffs) |
| 8 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001320683.1 | 68.9% | 67.2% | (many diffs) |
| 9 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_015605.8 | 68.9% | 67.2% | (many diffs) |
| 10 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001042361.4 | 64.4% | 62.3% | (many diffs) |
| 11 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352534.1 | 59.2% | 52.1% | (many diffs) |
| 12 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352541.1 | 58.8% | 42.9% | (many diffs) |
| 13 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352542.1 | 58.8% | 42.9% | (many diffs) |
| 14 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XM_017016062.1 | 58.8% | 42.9% | (many diffs) |
| 15 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352523.1 | 54% | 47.6% | (many diffs) |
| 16 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001042359.3 | 52.3% | 50.7% | (many diffs) |
| 17 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XM_017016048.1 | 46.3% | 45.2% | (many diffs) |
| 18 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XM_017016047.1 | 45.8% | 44.7% | (many diffs) |
| 19 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XM_017016046.1 | 44.7% | 43.6% | (many diffs) |
| 20 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001320682.1 | 42.4% | 5.9% | (many diffs) |
| 21 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001320689.1 | 42.4% | 5.9% | (many diffs) |
| 22 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001320690.1 | 42.4% | 5.9% | (many diffs) |
| 23 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352547.1 | 42.4% | 5.9% | (many diffs) |
| 24 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352548.1 | 42.4% | 5.9% | (many diffs) |
| 25 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352549.2 | 41.7% | 6.2% | (many diffs) |
| 26 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352550.1 | 41.7% | 6.2% | (many diffs) |
| 27 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XM_017016043.1 | 38.2% | 37.2% | (many diffs) |
| 28 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XM_017016042.1 | 37.8% | 36.8% | (many diffs) |
| 29 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352540.2 | 37.8% | 35.8% | (many diffs) |
| 30 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001042357.4 | 37.1% | 36.1% | (many diffs) |
| 31 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XM_011539605.2 | 37.1% | 36.1% | (many diffs) |
| 32 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XM_011539606.3 | 37.1% | 36.1% | (many diffs) |
| 33 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XM_011539607.2 | 37.1% | 36.1% | (many diffs) |
| 34 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352536.1 | 36.3% | 34.4% | (many diffs) |
| 35 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352537.1 | 36.3% | 34.4% | (many diffs) |
| 36 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352538.1 | 36.3% | 34.4% | (many diffs) |
| 37 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352539.1 | 36.3% | 34.4% | (many diffs) |
| 38 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001042358.4 | 34.7% | 33.5% | (many diffs) |
| 39 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001042364.4 | 33.1% | 31.4% | (many diffs) |
| 40 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352532.1 | 33.1% | 31.4% | (many diffs) |
| 41 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352533.1 | 33.1% | 31.4% | (many diffs) |
| 42 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001320685.1 | 29.3% | 25.9% | (many diffs) |
| 43 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352521.1 | 29.3% | 25.9% | (many diffs) |
| 44 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001320688.1 | 26.9% | 25.5% | (many diffs) |
| 45 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352545.1 | 25.6% | 20.8% | (many diffs) |
| 46 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352522.1 | 21.5% | 20.4% | (many diffs) |
| 47 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001352546.1 | 21.1% | 18.9% | (many diffs) |
| 48 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001042363.4 | 17.8% | 16.9% | (many diffs) |
| 49 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001320681.1 | 17.8% | 16.9% | (many diffs) |
| 50 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | NM_001320686.1 | 17.8% | 16.9% | (many diffs) |
| 51 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XM_011539610.2 | 17.8% | 16.9% | (many diffs) |
| 52 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XR_001747083.1 | 17.2% | (many diffs) | |
| 53 | human | 26095 | PTPN20 | protein tyrosine phosphatas... | XR_001747082.1 | 13.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttcacctagg gactttagag cagagcctgt aaacgattat gagggaaatg 121 actctgaagc agaagacttg aatttcaggg agactttgcc ttcatcaagt caggaaaaca 181 cacctagatc aaaggttttt gaaaataaag ttaattcaga gaaggtaaaa ctttctcTTC 241 GGAATTTCCC ACATAATGAT TATGAGGATG TTTTTGAAGA GCCTTCAGAA AGTGGCAGTG 301 ATCCCAGCAT GTGGACAGCC AGAGGCCCCT TCAGAAGAGA CAGGTGGAGC AGTGAGGATG 361 AGGAGGCTGC AGGGCCATCA CAGGCTCTCT CCCCTCTACT TTCTGGTACG CGCAAAATTG 421 TTTCTGAAGG AGAACTAGAT CAGTTGGCTC AGATTCGGCC ATTAATATTC AATTTTCATG 481 AGCAGACAGC CATCAAGGAT TGTTTGAAAA TCCTTGAAGA AAAAAACAGC AGCGTATGAC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GACACCATAG GAAAGCATTC ACTAGCACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt