Construct: ORF TRCN0000478294
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016831.1_s317c1
- Derived from:
- ccsbBroadEn_02601
- DNA Barcode:
- AAAGATATTTGATCCCACCCCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB31 (11031)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478294
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11031 | RAB31 | RAB31, member RAS oncogene ... | NM_006868.4 | 100% | 100% | |
| 2 | human | 11031 | RAB31 | RAB31, member RAS oncogene ... | XM_017025529.2 | 79.9% | 72% | 1_37del;76_185del |
| 3 | human | 11031 | RAB31 | RAB31, member RAS oncogene ... | XM_017025528.2 | 78.6% | 73.8% | 1_37del;76_197del |
| 4 | mouse | 106572 | Rab31 | RAB31, member RAS oncogene ... | NM_133685.2 | 88.3% | 94.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 651
- ORF length:
- 585
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggcgatacgg gagctcaaag tgtgccttct cggggacact ggggttggga 121 aatcaagcat cgtgtgtcga tttgtccagg atcactttga ccacaacatc agccctacta 181 ttggggcatc ttttatgacc aaaactgtgc cttgtggaaa tgaacttcac aagttcctca 241 tctgggacac tgctggtcag gaacggtttc attcattggc tcccatgtac tatcgaggcT 301 CAGCTGCAGC TGTTATCGTG TATGATATTA CCAAGCAGGA TTCATTTTAT ACCTTGAAGA 361 AATGGGTCAA GGAGCTGAAA GAACATGGTC CAGAAAACAT TGTAATGGCC ATCGCTGGAA 421 ACAAGTGCGA CCTCTCAGAT ATTAGGGAGG TTCCCCTGAA GGATGCTAAG GAATACGCTG 481 AATCCATAGG TGCCATCGTG GTTGAGACAA GTGCAAAAAA TGCTATTAAT ATCGAAGAGC 541 TCTTTCAAGG AATCAGCCGC CAGATCCCAC CCTTGGACCC CCATGAAAAT GGAAACAATG 601 GAACAATCAA AGTTGAGAAG CCAACCATGC AAGCCAGCCG CCGGTGCTGT TGCCCAACTT 661 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 721 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 781 CTTGTGGAAA GGACGAAAAG ATATTTGATC CCACCCCATA ACGCGTTAAG TCgacaatca 841 acctctggat tacaaaattt gtgaaagatt