Transcript: Mouse NM_133685.2

Mus musculus RAB31, member RAS oncogene family (Rab31), mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Rab31 (106572)
Length:
3476
CDS:
209..796

Additional Resources:

NCBI RefSeq record:
NM_133685.2
NBCI Gene record:
Rab31 (106572)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_133685.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000100438 CAGCGCGAAGAATGCCATTAA pLKO.1 652 CDS 100% 13.200 18.480 N Rab31 n/a
2 TRCN0000324830 CAGCGCGAAGAATGCCATTAA pLKO_005 652 CDS 100% 13.200 18.480 N Rab31 n/a
3 TRCN0000380682 CGCTAAGGAGTACGCTGAATC pLKO_005 607 CDS 100% 10.800 15.120 N Rab31 n/a
4 TRCN0000100439 CGCGAAGAATGCCATTAACAT pLKO.1 655 CDS 100% 5.625 7.875 N Rab31 n/a
5 TRCN0000100436 GCAGGATTCATTTCATACCTT pLKO.1 478 CDS 100% 3.000 4.200 N Rab31 n/a
6 TRCN0000324829 GCAGGATTCATTTCATACCTT pLKO_005 478 CDS 100% 3.000 4.200 N Rab31 n/a
7 TRCN0000379445 ACCGTGCCTTGTGGAAATGAA pLKO_005 347 CDS 100% 5.625 4.500 N Rab31 n/a
8 TRCN0000381460 TCAAGGAATCAGCCGCCAGAT pLKO_005 688 CDS 100% 4.050 3.240 N Rab31 n/a
9 TRCN0000100435 GCGGGTTTGTATATGTGTAAA pLKO.1 2742 3UTR 100% 13.200 9.240 N Rab31 n/a
10 TRCN0000324828 GCGGGTTTGTATATGTGTAAA pLKO_005 2742 3UTR 100% 13.200 9.240 N Rab31 n/a
11 TRCN0000381947 TGGCGATTGCTGGGAACAAGT pLKO_005 549 CDS 100% 4.950 3.465 N Rab31 n/a
12 TRCN0000100437 CCAGGATCACTTTGACCACAA pLKO.1 289 CDS 100% 4.050 2.835 N Rab31 n/a
13 TRCN0000324913 CCAGGATCACTTTGACCACAA pLKO_005 289 CDS 100% 4.050 2.835 N Rab31 n/a
14 TRCN0000380370 GGGTTGGGAAATCCAGCATTG pLKO_005 255 CDS 100% 6.000 3.600 N Rab31 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133685.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02601 pDONR223 100% 88.3% 94.3% None (many diffs) n/a
2 ccsbBroad304_02601 pLX_304 0% 88.3% 94.3% V5 (many diffs) n/a
3 TRCN0000478294 AAAGATATTTGATCCCACCCCATA pLX_317 59.8% 88.3% 94.3% V5 (many diffs) n/a
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