Construct: ORF TRCN0000478301
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011931.1_s317c1
- Derived from:
- ccsbBroadEn_07508
- DNA Barcode:
- TGCTCACCATTTGTTTCAGCACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM19 (9904)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478301
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9904 | RBM19 | RNA binding motif protein 19 | NM_001146698.2 | 99.7% | 99.4% | (many diffs) |
| 2 | human | 9904 | RBM19 | RNA binding motif protein 19 | NM_001146699.2 | 99.7% | 99.4% | (many diffs) |
| 3 | human | 9904 | RBM19 | RNA binding motif protein 19 | NM_016196.4 | 99.7% | 99.4% | (many diffs) |
| 4 | human | 9904 | RBM19 | RNA binding motif protein 19 | XM_017020280.1 | 97.2% | 96.9% | (many diffs) |
| 5 | human | 9904 | RBM19 | RNA binding motif protein 19 | XM_017020281.1 | 97.2% | 96.9% | (many diffs) |
| 6 | human | 9904 | RBM19 | RNA binding motif protein 19 | XR_944848.2 | 76.6% | (many diffs) | |
| 7 | human | 9904 | RBM19 | RNA binding motif protein 19 | XR_001748932.1 | 74.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2946
- ORF length:
- 2880
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gcgactgatc gtgaagaatc tcccgaatgg gatgaaggag gagcgtttca 121 ggcagctgtt tgccgccttc ggcacgctga cagactgcag cctgaagttc accaaagatg 181 gcaagttccg caagtttggt tttattggct tcaagtccga ggaagaggcc cagaaggcac 241 agaagcattt caacaagagc ttcatcgaca catcccggat cacagtggag ttctgcaagt 301 cattcgggga cccggccaaa cccagagcct ggagcaaaca tgcccagaaa ccaagccagc 361 ccaagcagcc tccaaaagac tctactactc cagaaattaa gaaagatgag aagaagaaaa 421 aggtggcagg tcaactggag aagctgaagg aggatacaga gttccaggag tttctgtcag 481 ttcatcagag gcgggcgcag gcagccactt gggcgaatga tggcctggat gctgagccct 541 cgaaagggaa gagcaagccg gccagtgact acctgaactt cgactccgat tctgggcagg 601 agagtgagga ggagggagcc ggggaggacc tggaagaaga ggcaagcctc gaaccaaagg 661 cagctgtgca gaaggagctg tcggacatgg attacctgaa atccaagatg gtgaaggctg 721 ggtcgtcctc ttcctcggag gaagaggaaa gtgaagatga agccgtgcac tgtgatgaag 781 ggagtgaggc cgaggaagag gattcctccg ccaccccagt cctgcaggaa agagacagca 841 ggggtgcagg ccaagagcaa gggatgccag ctgggaaaaa gagaccaccg gaggccagag 901 ccgagacaga gaaaccagca aaccagaagg aacccaccac ctgccacacc gtgaagctgc 961 ggggagcccc gttcaatgtc acagagaaaa atgttatgga attcctggca cccctgaaac 1021 cagtggccat tcgaattgtg agaaacgctc atgggaataa aacaggatac atctttgtgg 1081 atttcagcaa tgaagaggaa gtgaagcaag ctctgaaatg caaccgggag tacatgggtg 1141 ggcgctacat cgaggtgttc agggaaaaga acgtccccac caccaagggt gcaccaaaga 1201 ataccaccaa atcctggcaa ggccggatac tcggggagaa cgaagaggag gaggacctgg 1261 ccgaatccgg aaggctcttt gtacggaacc tgccctacac cagcaccgag gaggatctgg 1321 agaagctctt ctccaaatac ggtcccctgt ctgagctcca ctaccccatc gacagcctga 1381 ccaagaaacc caagggtttt gcattcatca ccttcatgtt ccctgagcac gctgtgaagg 1441 cctactcgga ggtggacggg caggtattcc agggcaggat gctccacgtg ttaccatcta 1501 ccatcaagaa ggaagccagc gaggatgcca gtgccctggg atcgtcgtcc tacaagaaga 1561 agaaggaggc ccaggacaaa gccaacagtg ccagctctca caactggaac acactattca 1621 tggggccgaa tgccgtggcc gatgccatcg cacagaagta caacgccacc aaaagtcaag 1681 tgtttgacca cgagaccaag ggcagcgtgg ccgtgcgcgt ggctctgggg gaaacccagc 1741 tcgtccagga agtgcggcgt tttctcatag acaacggggt cagcctggat tccttcagcc 1801 aggctgcagc agagcgaagc aagactgtga ttctggtcaa gaacctcccg gcaggcaccc 1861 tggcggccga gctgcaggag accttcggcc gttttggcag cctgggccgc gtgctgctgc 1921 cagagggcgg aaccactgcc atcgtggagt tcctggagcc cctggaggcc cgcaaggcct 1981 tcaggcatct ggcctattcc aagttccatc atgtccccct ctatctggag tgggctccag 2041 ttggcgtctt ctccagcgca gccccacaga agaaaaagct ccaagacaca ccttcagaac 2101 ccatggaaaa ggacccagca gagccagaaa cagtgcctga tggcgaaacc ccagaagatg 2161 aaaatccaac agaggaagga gcagacaact cttcagcaaa gatggaagag gaggaggagg 2221 aagaggaaga agaagaagag agcctcccag gatgtactct gtttattaag aatctcaatt 2281 ttgacacaac agaagagaag ctgaaggaag tgttttcaaa agtggggaca gtgaagagct 2341 gctccatctc caagaagaag aacaaagcag gagtgctcct ttccatgggg tttggatttg 2401 tggaatacag gaagccggag caagcccaga aagctctcaa gcagctccag ggtcacgtcg 2461 tggacggcca caagctggaa gtgaggatct cggaacgagc cactaagcca gccgtgacat 2521 tggctcggaa gaaacaagtt cccagaaagc agaccacctc caagatcctg gtgcggaaca 2581 tccccttcca ggcccacagc cgggagatcc gagagctctt cagcaccttt ggggagttga 2641 agacggTCCG CCTGCCAAAG AAGATGACTG GGACAGGCAC ACACAGAGGC TTCGGCTTTG 2701 TGGACTTCCT CACCAAGCAG GATGCGAAGA GAGCCTTCAA CGCCCTGTGT CACAGCACCC 2761 ACTTGTACGG GCGGAGGCTG GTGCTGGAGT GGGCCGACTC CGAGGTGACC CTGCAGGCCC 2821 TGCGGCGGAA GACGGCCGCT CACTTTCACG AGCCCCCGAA GAAAAAGCGG TCTGTGGTGT 2881 TGGACGAGAT CCTGGAGCAG CTGGAAGGCA GTGACAGCGA CAGCGAGGAG CAGACCCTTC 2941 AGCTGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 3001 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 3061 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGCTCACCA TTTGTTTCAG CACGCACGCG 3121 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt