Transcript: Human NM_016196.4

Homo sapiens RNA binding motif protein 19 (RBM19), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-06-08
Taxon:
Homo sapiens (human)
Gene:
RBM19 (9904)
Length:
4148
CDS:
99..2981

Additional Resources:

NCBI RefSeq record:
NM_016196.4
NBCI Gene record:
RBM19 (9904)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_016196.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000005067 CCCAGGATGTACTCTGTTTAT pLKO.1 2279 CDS 100% 13.200 18.480 N RBM19 n/a
2 TRCN0000005068 CGGACATGGATTACCTGAAAT pLKO.1 715 CDS 100% 13.200 18.480 N RBM19 n/a
3 TRCN0000273131 ACTAAGCCAGCCGTGACATTG pLKO_005 2535 CDS 100% 10.800 15.120 N RBM19 n/a
4 TRCN0000273129 ACTCTACTACTCCAGAAATTA pLKO_005 412 CDS 100% 15.000 10.500 N RBM19 n/a
5 TRCN0000005066 GAGAGAAATAAACGCAGTAAT pLKO.1 3486 3UTR 100% 13.200 9.240 N RBM19 n/a
6 TRCN0000273130 TAGCTAGTTCTTACTTCATAA pLKO_005 3458 3UTR 100% 13.200 9.240 N RBM19 n/a
7 TRCN0000273197 ATGGCAAGTTCCGCAAGTTTG pLKO_005 211 CDS 100% 10.800 7.560 N RBM19 n/a
8 TRCN0000005069 CCGTTCAATGTCACAGAGAAA pLKO.1 1002 CDS 100% 4.950 3.465 N RBM19 n/a
9 TRCN0000005070 GCCTATTCCAAGTTCCATCAT pLKO.1 2025 CDS 100% 4.950 3.465 N RBM19 n/a
10 TRCN0000273200 GCCTATTCCAAGTTCCATCAT pLKO_005 2025 CDS 100% 4.950 3.465 N RBM19 n/a
11 TRCN0000177221 GAGGAAGAAGAAGAAGAGATA pLKO.1 2256 CDS 100% 4.950 2.475 Y Cnpy4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_016196.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07508 pDONR223 100% 99.7% 99.4% None (many diffs) n/a
2 ccsbBroad304_07508 pLX_304 0% 99.7% 99.4% V5 (many diffs) n/a
3 TRCN0000478301 TGCTCACCATTTGTTTCAGCACGC pLX_317 10.6% 99.7% 99.4% V5 (many diffs) n/a
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