Construct: ORF TRCN0000478304
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006469.2_s317c1
- Derived from:
- ccsbBroadEn_15797
- DNA Barcode:
- GTACTCAGGTGTCGGGTGAAGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EML4 (27436)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478304
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27436 | EML4 | EMAP like 4 | XR_001738709.1 | 3.3% | (many diffs) | |
| 2 | human | 27436 | EML4 | EMAP like 4 | XR_001738708.1 | 3.3% | (many diffs) | |
| 3 | human | 27436 | EML4 | EMAP like 4 | XR_001738710.1 | 3.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 276
- ORF length:
- 186
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGGCTATAC TCCCTGCAAG AAATATACTG 121 ACATGAACAG GCAGTTCTTG GAGAAGAAAG AGCATTTCTT TAAGTACCTG GGGAATACAG 181 CTCTCAGTGA TCAGCAGGGA GTTTATTTGA GGACATCAGT CACCTTTGGG GTTGCCATGT 241 ACAATGAGAT TTATAATCCT GATACTCTTC GGTGGTACCC AACTTTCTTG TACAAAGTGG 301 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 361 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 421 AGTACTCAGG TGTCGGGTGA AGGCCACGCG TTAAGTCgac aatcaacctc tggattacaa 481 aatttgtgaa agatt