Construct: ORF TRCN0000478317
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017131.1_s317c1
- Derived from:
- ccsbBroadEn_07556
- DNA Barcode:
- GATACCTACCAGGCAGTCATCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAL4 (10126)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478317
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10126 | DNAL4 | dynein axonemal light chain 4 | NM_005740.3 | 99.6% | 100% | 36T>C |
| 2 | mouse | 54152 | Dnal4 | dynein, axonemal, light cha... | NM_017470.2 | 93.9% | 99% | (many diffs) |
| 3 | mouse | 54152 | Dnal4 | dynein, axonemal, light cha... | XM_006521170.2 | 93.9% | 99% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 381
- ORF length:
- 315
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg agaaacagaa gggaagaaag atgaggctga ctataagcga ctgcagaccT 121 TCCCTCTGGT CAGGCACTCG GACATGCCAG AGGAGATGCG CGTGGAGACC ATGGAGCTAT 181 GTGTCACAGC CTGTGAGAAA TTCTCCAACA ACAACGAGAG CGCCGCCAAG ATGATCAAAG 241 AGACAATGGA CAAGAAGTTC GGCTCCTCCT GGCACGTGGT GATCGGCGAG GGCTTTGGGT 301 TTGAGATCAC CCACGAGGTG AAGAACCTCC TCTACCTGTA CTTCGGGGGC ACCCTGGCTG 361 TGTGCGTCTG GAAGTGCTCC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGATA CCTACCAGGC 541 AGTCATCGCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt