Transcript: Mouse XM_006521170.2

PREDICTED: Mus musculus dynein, axonemal, light chain 4 (Dnal4), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dnal4 (54152)
Length:
1524
CDS:
299..616

Additional Resources:

NCBI RefSeq record:
XM_006521170.2
NBCI Gene record:
Dnal4 (54152)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006521170.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000100285 CGGGATTCTTCTCCTTGAAAT pLKO.1 879 3UTR 100% 13.200 10.560 N Dnal4 n/a
2 TRCN0000351822 CGGGATTCTTCTCCTTGAAAT pLKO_005 879 3UTR 100% 13.200 10.560 N Dnal4 n/a
3 TRCN0000364029 GGCTGACTATAAACGGCTGCA pLKO_005 328 CDS 100% 2.160 1.728 N Dnal4 n/a
4 TRCN0000219808 GTGTCACAGCCTGTGAGAAAT pLKO.1 414 CDS 100% 13.200 9.240 N DNAL4 n/a
5 TRCN0000100286 CAACAATGAGAGTGCTGCAAA pLKO.1 442 CDS 100% 4.950 3.465 N Dnal4 n/a
6 TRCN0000100288 CAAGGAGACAATGGACAAGAA pLKO.1 469 CDS 100% 4.950 3.465 N Dnal4 n/a
7 TRCN0000351821 CAAGGAGACAATGGACAAGAA pLKO_005 469 CDS 100% 4.950 3.465 N Dnal4 n/a
8 TRCN0000100289 CTGTGAGAAATTCTCCAACAA pLKO.1 424 CDS 100% 4.950 3.465 N Dnal4 n/a
9 TRCN0000348692 GAACCTCCTGTACCTGTACTT pLKO_005 556 CDS 100% 4.950 3.465 N Dnal4 n/a
10 TRCN0000100287 TGAGAAATTCTCCAACAACAA pLKO.1 427 CDS 100% 4.950 3.465 N Dnal4 n/a
11 TRCN0000351740 TGAGAAATTCTCCAACAACAA pLKO_005 427 CDS 100% 4.950 3.465 N Dnal4 n/a
12 TRCN0000376976 GTTTGAGATCACCCACGAGGT pLKO_005 532 CDS 100% 2.160 1.512 N Dnal4 n/a
13 TRCN0000376897 TGTGTGTGGAAGTGCTCCTGA pLKO_005 596 CDS 100% 2.640 1.584 N Dnal4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006521170.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07556 pDONR223 100% 93.9% 99% None (many diffs) n/a
2 ccsbBroad304_07556 pLX_304 0% 93.9% 99% V5 (many diffs) n/a
3 TRCN0000478317 GATACCTACCAGGCAGTCATCGCA pLX_317 81.1% 93.9% 99% V5 (many diffs) n/a
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