Construct: ORF TRCN0000478320
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016798.2_s317c1
- Derived from:
- ccsbBroadEn_11148
- DNA Barcode:
- ACACATACCAAAACTCGCTCTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SOD2 (6648)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478320
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6648 | SOD2 | superoxide dismutase 2 | NM_001322816.1 | 99.7% | 99.2% | 47T>C |
| 2 | human | 6648 | SOD2 | superoxide dismutase 2 | NM_001024466.3 | 60.3% | 44.4% | (many diffs) |
| 3 | human | 6648 | SOD2 | superoxide dismutase 2 | NM_001322814.2 | 60.3% | 44.4% | (many diffs) |
| 4 | human | 6648 | SOD2 | superoxide dismutase 2 | NM_000636.4 | 52.8% | 36.7% | (many diffs) |
| 5 | human | 6648 | SOD2 | superoxide dismutase 2 | NM_001024465.3 | 52.8% | 36.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 486
- ORF length:
- 420
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gagccgggca gtgtgcggca ccagcaggca gctggctccg gctttggggt 121 atctgggctc caggcagaag cacagcctcc ccgacctgcc ctacgactac ggcgccctgg 181 aacctcacat caacgcgcag atcatgcagc tgcaccacag caagcaccac gcggccTACG 241 TGAACAACCT GAACGTCACC GAGGAGAAGT ACCAGGAGGC GTTGGCCAAG GGTAGGTTCC 301 AGGCTGAGCG GCGGGAGGCA GTCCCCGGCA GAGGCGACCC CAGGGAGCCA GGCCCCATAC 361 GGACGGGCCT CTCCGTGGAG GAGAACTCGC TTCGTATTTG TACCGGTTCC GAGTTTTCCA 421 GGCACGATAG TCTCTCTTTT AAACACATGG TCTACCTCAT TGTAGAAGGA GTGCCTCGAT 481 GGGTTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 541 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 601 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACACATACC AAAACTCGCT CTCCTACGCG 661 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt