Construct: ORF TRCN0000478328
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001580.1_s317c1
- Derived from:
- ccsbBroadEn_04507
- DNA Barcode:
- CACTACCTCATGAAATGAGAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYS1 (90196)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478328
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90196 | SYS1 | SYS1 golgi trafficking protein | NM_001197129.2 | 100% | 100% | |
| 2 | human | 90196 | SYS1 | SYS1 golgi trafficking protein | NM_033542.4 | 100% | 100% | |
| 3 | human | 90196 | SYS1 | SYS1 golgi trafficking protein | NM_001099791.3 | 48.7% | 48.7% | 228_229ins240 |
| 4 | mouse | 66460 | Sys1 | SYS1 Golgi-localized integr... | NM_025575.3 | 94.2% | 97.4% | (many diffs) |
| 5 | mouse | 66460 | Sys1 | SYS1 Golgi-localized integr... | XM_011239732.3 | 94.2% | 97.4% | (many diffs) |
| 6 | mouse | 66460 | Sys1 | SYS1 Golgi-localized integr... | XM_011239733.2 | 94.2% | 97.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 534
- ORF length:
- 468
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggtcagttc cgcagctacg tgtgggaccc gctgctgatc ctgtcgcaga 121 tcgtcctcat gcagaccgtg tattacggct cgctgggcct gtggctggcg ctggtggacg 181 ggctagtgcg aagcagcccc tcgctggacc agatgttcga cgccgagatc ctgggctttt 241 ccaccccTCC AGGCCGGCTC TCCATGATGT CCTTCATCCT CAACGCCCTC ACCTGTGCCC 301 TGGGCTTGCT GTACTTCATC CGGCGAGGAA AGCAGTGTCT GGATTTCACT GTCACTGTCC 361 ATTTCTTTCA CCTCCTGGGC TGCTGGTTCT ACAGCTCCCG TTTCCCCTCG GCGCTGACCT 421 GGTGGCTGGT CCAAGCCGTG TGCATTGCAC TCATGGCTGT CATCGGGGAG TACCTGTGCA 481 TGCGGACGGA GCTCAAGGAG ATACCCCTCA ACTCAGCCCC TAAATCCAAT GTCTACCCAA 541 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 601 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 661 TATCTTGTGG AAAGGACGAC ACTACCTCAT GAAATGAGAA CCAACGCGTT AAGTCgacaa 721 tcaacctctg gattacaaaa tttgtgaaag att