Construct: ORF TRCN0000478346
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002629.2_s317c1
- Derived from:
- ccsbBroadEn_06505
- DNA Barcode:
- GCTAGCTAAGGGCTCACTTAAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT7 (3855)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478346
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3855 | KRT7 | keratin 7 | NM_005556.4 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 3855 | KRT7 | keratin 7 | XM_011538325.2 | 89.8% | 82.6% | (many diffs) |
| 3 | human | 3855 | KRT7 | keratin 7 | XR_001748699.2 | 75.2% | (many diffs) | |
| 4 | human | 3855 | KRT7 | keratin 7 | XM_017019294.1 | 60.9% | 57.9% | (many diffs) |
| 5 | human | 3855 | KRT7 | keratin 7 | XR_001748700.2 | 56.2% | (many diffs) | |
| 6 | mouse | 110310 | Krt7 | keratin 7 | NM_033073.3 | 83.4% | 81.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1473
- ORF length:
- 1407
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc catccacttc agctccccgg tattcacctc gcgctcagcc gccttctcgg 121 gccgcggcgc ccaggtgcgc ctgagctccg ctcgccccgg cggccttggc agcagcagcc 181 tctacggcct cggcgcctcg cggccgcgcg tggccgtgcg ctctgcctat gggggcccgg 241 tgggcgccgg catccgcgag gtcaccatta accagagcct gctggccccg ctgcggctgg 301 acgccgaccc ctccctccag cgggtgcgcc aggaggagag cgagcagatc aagaccctca 361 acaacaagtt tgcctccttc atcgacaagg tgcggtttct ggagcagcag aacaagctgc 421 tggagaccaa gtggacgctg ctgcaggagc agaagtcggc caagagcagc cgcctcccag 481 acatctttga ggcccagatt gctggccttc ggggtcagct tgaggcactg caggtggatg 541 ggggccgcct ggaggcggag ctgcggagca tgcaggatgt ggtggaggac ttcaagaata 601 agtacgaaga tgaaattaac cgccgcacag ctgctgagaa tgagtttgtg gtgctgaaga 661 aggatgtgga tgctgcctac atgagcaagg tggagctgga ggccaaggtg gatgccctga 721 atgatgagat caacttcctc aggaccctca atgagacgga gttgacagag ctgcagtccc 781 agatctccga cacatctgtg gtgctgtcca tggacaacag tcgctccctg gacctggacg 841 gcatcatcgc tgaggtcaag gcacagtatg aggagatggc caaatgcagc cgggctgagg 901 ctgaagcctg gtaccagacc aagtttgaga ccctccaggc ccaggctggg aagcatgggg 961 acgacctccg gaatacccgg aatgagattt cagagatgaa ccgggccatc cagaggctgc 1021 aggctgagat cgacaacatc aagaaccagc gtgccaagtt ggaggccgcc attgccgagg 1081 ctgaggagcg tggggagctg gcgctcaagg atgctcgtgc caagcaggag gagctggaag 1141 ccgcccTGCA GCGGGCCAAG CAGGATATGG CACGGCAGCT GCGTGAGTAC CAGGAACTCA 1201 TGAGCGTGAA GCTGGCCCTG GACATCGAGA TCGCCACCTA CCGCAAGCTG CTGGAGGGCG 1261 AGGAGAGCCG GTTGGCTGGA GATGGAGTGG GAGCCGTGAA TATCTCTGTG ATGAATTCCA 1321 CTGGTGGCAG TAGCAGTGGC GGTGGCATTG GGCTGACCCT CGGGGGAACC ATGGGCAGCA 1381 ATGCCCTGAG CTTCTCCAGC AGTGCGGGTC CTGGGCTCCT GAAGGCTTAT TCCATCCGGA 1441 CCGCATCCGC CAGTCGCAGG AGTGCCCGCG ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1501 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1561 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC 1621 TAGCTAAGGG CTCACTTAAA AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1681 ttgtgaaaga tt