Construct: ORF TRCN0000478373
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006785.1_s317c1
- Derived from:
- ccsbBroadEn_13349
- DNA Barcode:
- TACCCGACCCATGCGGACCGACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DACT2 (168002)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478373
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 168002 | DACT2 | dishevelled binding antagon... | NM_001286350.2 | 60.5% | 60.5% | 1_714del |
| 2 | human | 168002 | DACT2 | dishevelled binding antagon... | XM_011535507.2 | 56.8% | 56.8% | 1_834del |
| 3 | human | 168002 | DACT2 | dishevelled binding antagon... | NR_104425.2 | 54.7% | 1_377del;1476_2006del | |
| 4 | human | 168002 | DACT2 | dishevelled binding antagon... | NM_214462.5 | 47.2% | 47.2% | 1_1224del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1167
- ORF length:
- 1098
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccccttgag ggtccccagc agtctggcag ccttccagag gagggctcca 121 agccctcaaa cagctgtgtc ctcagggaga ccatggtgca ggcttctccc agctcaaagg 181 cccagcagac accctcagct caggactatg gacgaggcaa catcatatcc ccatccagga 241 tgctggacaa gagcccctca ccggcctctg ggcactttgc ccacccatcc tttgctgcca 301 gcctgaaaat gggtcccccc aagagcaagg ctgaaaaaat caagagaagt cccatggaca 361 aggtgctgag gtttgcaagg cagccgctgc ttctactgga caggcctgag ggagcccatg 421 cagcccccca gccatccctg gagtgggacc ctgcccactg gcccacaggg aggggcgggc 481 tccagcggag gccagccctg gcctgggagg cacccgggcg ctcctgttct gagtccaccc 541 tctaccccat gcctgtcctc gtccccttgg cagtggcccc gcaggagagc caccggacct 601 cagcccaagc cctgttcccc tttgaggcgt cactgctcac ctcagtggcc aggaggaagc 661 atcgccgctg gcagtccacc gtggagatct cggcccgggc ccgcctggcc agctgtcctg 721 agtctaacct ggggcccccc aggcccgtgg ccaggagagc aggtggccca ctggcccggg 781 gccgtccctc actggtccgc caggacgcct acaccaggag cgactcagag ccctccaagc 841 acTCGGCCGA GTGTGACCCG CGGTTCCCGT CAGTCATCCC GGAGACCAGC GAGGGAGAGT 901 CCAGTGACCA CACCACCAAC CGATTCGGAG ACCGTGAGTC CAGCAGCAGC GACGAGGAGG 961 GCGGCGCCCA GAGCAGGGAC TGTGACCTGG CACTGGGCTA TGTGGCGGCC GGGCATGCGG 1021 AGCTGGCCTG GACCCAGGAG GCCCCGGTCA GCTCGGGGCC ACTCCTGTCC CCCGTGCCCA 1081 AGCTGTGCCG TATTAAGGCC TCCAAGGCCC TGAAGAAGAA GATCCGCAGG TTCCAGCCGA 1141 CGGCCCTGAA GGTCATGACC ATGGTGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1201 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1261 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATACCCGAC 1321 CCATGCGGAC CGACCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1381 aagatt