Transcript: Human NR_104425.2

Homo sapiens dishevelled binding antagonist of beta catenin 2 (DACT2), transcript variant 4, non-coding RNA.

Source:
NCBI, updated 2019-08-13
Taxon:
Homo sapiens (human)
Gene:
DACT2 (168002)
Length:
2006
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_104425.2
NBCI Gene record:
DACT2 (168002)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_104425.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000428343 GTGCCCAAGCTGTGCCGTATT pLKO_005 1383 3UTR 100% 3.600 5.040 N DACT2 n/a
2 TRCN0000436055 ATGGACGAGGCAACATCATAT pLKO_005 517 3UTR 100% 13.200 9.240 N DACT2 n/a
3 TRCN0000424366 TCCTGTGCATGTCTGGGTTTC pLKO_005 1537 3UTR 100% 6.000 4.200 N DACT2 n/a
4 TRCN0000148739 CATGCACCTTGTTTCCATGTT pLKO.1 1761 3UTR 100% 4.950 3.465 N DACT2 n/a
5 TRCN0000180335 CAAGAGAAGTCCCATGGACAA pLKO.1 650 3UTR 100% 4.050 2.835 N DACT2 n/a
6 TRCN0000149172 GTTTCCCAATCCTTTGACCAT pLKO.1 1833 3UTR 100% 2.640 1.848 N DACT2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_104425.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13349 pDONR223 100% 54.7% None 1_377del;1476_2006del n/a
2 ccsbBroad304_13349 pLX_304 0% 54.7% V5 1_377del;1476_2006del n/a
3 TRCN0000478373 TACCCGACCCATGCGGACCGACCC pLX_317 29.8% 54.7% V5 1_377del;1476_2006del n/a
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