Construct: ORF TRCN0000478375
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010120.1_s317c1
- Derived from:
- ccsbBroadEn_14037
- DNA Barcode:
- CCCCGTCGCAAGCAACGATCCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPAN32 (10077)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478375
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10077 | TSPAN32 | tetraspanin 32 | NM_139022.2 | 80.4% | 69.4% | (many diffs) |
| 2 | human | 10077 | TSPAN32 | tetraspanin 32 | XM_011519822.1 | 73.4% | 67.1% | (many diffs) |
| 3 | human | 10077 | TSPAN32 | tetraspanin 32 | XM_011519818.3 | 72.7% | 61.7% | (many diffs) |
| 4 | human | 10077 | TSPAN32 | tetraspanin 32 | XM_005252720.4 | 71.7% | 64% | (many diffs) |
| 5 | human | 10077 | TSPAN32 | tetraspanin 32 | XM_005252719.4 | 68% | 57.2% | (many diffs) |
| 6 | human | 10077 | TSPAN32 | tetraspanin 32 | XM_011519815.3 | 67.8% | 55.8% | (many diffs) |
| 7 | human | 10077 | TSPAN32 | tetraspanin 32 | XM_011519819.1 | 66.2% | 54.4% | (many diffs) |
| 8 | human | 10077 | TSPAN32 | tetraspanin 32 | XM_017017067.1 | 66.2% | 54.4% | (many diffs) |
| 9 | human | 10077 | TSPAN32 | tetraspanin 32 | XM_017017065.1 | 55.8% | 31.1% | (many diffs) |
| 10 | human | 10077 | TSPAN32 | tetraspanin 32 | XM_017017064.1 | 52.7% | 28.4% | (many diffs) |
| 11 | human | 10077 | TSPAN32 | tetraspanin 32 | XM_011519829.3 | 52.4% | 44.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 840
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gccttggagt cgagtcaggg ttgccaaatg ccagatgctg gtcacctgct 121 tctttatctt gctgctgggc ctctctgtgg ccaccatggt gactcttacc tacttcgggg 181 cccactttgc tgtcatccgc cgagcgtccc tggagaagaa cccgtaccag gctgtgcacc 241 aatgggcctt ctctgcgggg ttgagcctgg tgggcctcct gactctggga gccgtgctga 301 gcgctgcagc caccgtgagg gaggcccagg gcctcatggc agggggcttc ctgtgcttct 361 ccctggcgtt ctgtgcacag gtgcaggtgg tgttctggag actccacagc cccacccagg 421 tggaggacgc catgctggac acctacgacc tggtatatga gcaggcgatg aaaggtacgt 481 cccacgtccg gcggcaggag ctggcggCCA TCCAGGACGT GTTTCTGTGC TGTGGGAAGA 541 AGTCTCCTTT CAGCCGTCTG GGGAGCACAG AGGCTGACCT GTGTCAGGGA GAGGAGGCGG 601 CGAGAGAGGA CTGCCTTCAG GGCATCCGGA GCTTCCTGAG GACACACCAG CAGGTCGCCT 661 CCAGCCTGAC CAGCATCGGC CTGGCCCTCA CGCTTGGACC GCAAGGGCAA ATACACCCTG 721 ACCCCACGAG CATGTGGCCG CCAGCCCCAG GAGCCCAGCC TCTTGAGATG CTCCCAGGGT 781 GGACCCACAC ATTGTCTCCA CTCCGAAGCA GTTGCTATTG GTCCAAGAGG ATACTCGGGT 841 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTGTGGAAAG GACGACCCCG TCGCAAGCAA CGATCCACCA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt