Construct: ORF TRCN0000478391
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009656.1_s317c1
- Derived from:
- ccsbBroadEn_12922
- DNA Barcode:
- CTGCCTAATTGCGACCAGTAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WNT3A (89780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478391
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89780 | WNT3A | Wnt family member 3A | NM_033131.4 | 91.4% | 91.4% | 1056_1057ins99 |
| 2 | human | 89780 | WNT3A | Wnt family member 3A | XM_011544319.3 | 84.7% | 78.2% | (many diffs) |
| 3 | mouse | 22416 | Wnt3a | wingless-type MMTV integrat... | NM_009522.2 | 80% | 87.7% | (many diffs) |
| 4 | mouse | 22415 | Wnt3 | wingless-type MMTV integrat... | XM_006533168.1 | 52% | 56.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1224
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccccactc ggatacttct tactcctctg cagcctgaag caggctctgg 121 gcagctaccc gatctggtgg tcgctggctg ttgggccaca gtattcctcc ctgggctcgc 181 agcccatcct gtgtgccagc atcccgggcc tggtccccaa gcagctccgc ttctgcagga 241 actacgtgga gatcatgccc agcgtggccg agggcatcaa gattggcatc caggagtgcc 301 agcaccagtt ccgcggccgc cggtggaact gcaccaccgt ccacgacagc ctggccatct 361 tcgggcccgt gctggacaaa gctaccaggg agtcggcctt tgtccacgcc attgcctcag 421 ccggtgtggc ctttgcagtg acacgctcat gtgcagaagg cacggccgcc atctgtggct 481 gcagcagccg ccaccagggc tcaccaggca agggctggaa gtggggtggc tgtagcgagg 541 acatcgagtt tggtgggatg gtgtctcggg agttcgccga cgcccgggag aaccggccag 601 atgcccgctc agccatgaac cgccacaaca acgaggctgg gcgccaggcc atcgccagcc 661 acatgcacct caagtgcaag tgccacgggc tgtcgggcag ctgcgaggtg aagacatgct 721 ggtggtcgca acccgacttc cgcgccatcg gtgacttcct caaggacaag tacgacagcg 781 cctcggagat ggtggtggag aagcaccggg agtcccgcgg ctgggtggag accctgcggc 841 cgcgctacac ctacttcaag gtgcccacgg agcgcgacct ggtctactac gaggcctcgc 901 ccaacttctg cgagcccaac cctgagacgg gctccTTCGG CACGCGCGAC CGCACCTGCA 961 ACGTCAGCTC GCACGGCATC GACGGCTGCG ACCTGCTGTG CTGCGGCCGC GGCCACAACG 1021 CGCGAGCGGA GCGGCGCCGG GAGAAGTGCC GCTGCGTGTT CCACTGGTGC TGCTACGTCA 1081 GCTGCCAGGA GTGCACGCGC GTCTACGACG TGCACACCTG CAAGAATCCC GGCTCCAGAG 1141 CAGGAAATTC AGCCCACCAG CCACCTCATC CCCAACCCCC TGTAAGGTTC CATCCACCCC 1201 TGCGTCGAGC TGGGAAGGTT CCATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1261 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1321 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TGCCTAATTG 1381 CGACCAGTAA CCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1441 att