Construct: ORF TRCN0000478407
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004205.1_s317c1
- Derived from:
- ccsbBroadEn_10083
- DNA Barcode:
- GCACAAGCCTGAAGCTTCGCTAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSUN4 (387338)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478407
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 387338 | NSUN4 | NOP2/Sun RNA methyltransfer... | NM_199044.3 | 99.7% | 99.4% | 6T>A;151A>G;1010A>G |
| 2 | human | 387338 | NSUN4 | NOP2/Sun RNA methyltransfer... | NM_001256127.2 | 87% | 86.7% | 0_1ins147;4A>G;863A>G |
| 3 | human | 387338 | NSUN4 | NOP2/Sun RNA methyltransfer... | NM_001256128.2 | 87% | 86.7% | 0_1ins147;4A>G;863A>G |
| 4 | human | 387338 | NSUN4 | NOP2/Sun RNA methyltransfer... | NR_045789.1 | 21.6% | (many diffs) | |
| 5 | human | 387338 | NSUN4 | NOP2/Sun RNA methyltransfer... | NR_045790.1 | 19.3% | (many diffs) | |
| 6 | human | 387338 | NSUN4 | NOP2/Sun RNA methyltransfer... | NR_045791.1 | 15.8% | (many diffs) | |
| 7 | mouse | 72181 | Nsun4 | NOL1/NOP2/Sun domain family... | NM_028142.4 | 85.9% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1218
- ORF length:
- 1152
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcgctgaca ctgaggggtg tccgggagct gctgaagcgt gtggacctcg 121 cgacggtccc gcggagacat cgatataaga agaaatgggc tgccacagag cccaaattcc 181 ctgctgttcg actggctttg cagaattttg acatggctta cagtgtgcag tttggagatc 241 tttggccatc aatccgtgtc agtctcctct cagagcagaa gtatggtgca ctggtcaata 301 actttgctgc ctgggatcat gtaagtgcta agctggagca gctgagtgcc aaggattttg 361 tgaatgaagc catctcccac tgggaactgc agtctgaggg tggccaatct gcagccccat 421 cccctgcctc ctgggcctgc agtccgaacc ttcgatgctt cacttttgac agaggggata 481 tcagtcgctt ccctcctgcc agacctggca gcctgggtgt catggagtac tacctgatgg 541 atgctgcctc cttgctgcct gttctggccc tcggcctgca gcctggggac atcgtgcttg 601 acctatgtgc agctcctggg ggaaagacac tagcgttgct tcagactggc tgttgccgca 661 atcttgctgc caatgatctc tccccgtccc gaatagccag actacagaag atccttcaca 721 gctatgtgcc tgaagagatc agggatggaa atcaagttcg agttacctca tgggatggca 781 ggaaatgggg agaactggag ggggacacct atgaccgggt gctggtggat gtgccctgta 841 ccacagaccg ccactccctt catgaggagg agaacaacaT CTTTAAGCGG TCAAGGAAGA 901 AGGAGCGACA GATATTGCCT GTGCTGCAAG TGCAGCTTCT TGCGGCTGGA CTCCTTGCCA 961 CCAAACCAGG AGGCCATGTT GTCTATTCTA CCTGCTCACT CTCACACTTA CAGAACGAGT 1021 ATGTGGTGCA AGGTGCCATT GAGCTCCTGG CCAATCAATA CAGCATCCAG GTACGGGTGG 1081 AAGATCTGAC TCACTTCCGA AGGGTTTTCA TGGACACATT TTGTTTCTTC TCATCCTGTC 1141 AGGTTGGGGA GCTGGTAATA CCAAACCTCA TGGCCAATTT TGGCCCCATG TACTTCTGCA 1201 AAATGCGTAG GCTGACATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1261 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1321 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCACAAG CCTGAAGCTT 1381 CGCTAGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt