Construct: ORF TRCN0000478431

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004883.1_s317c1
Derived from:
ccsbBroadEn_01982
DNA Barcode:
GTAAGTACACCCCTAATCTCTATT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EIF3C (8663)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478431
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8663 EIF3C eukaryotic translation init... NM_001037808.2 100% 100%
2 human 8663 EIF3C eukaryotic translation init... NM_001199142.1 100% 100%
3 human 8663 EIF3C eukaryotic translation init... NM_001267574.2 100% 100%
4 human 8663 EIF3C eukaryotic translation init... NM_003752.4 100% 100%
5 human 728689 EIF3CL eukaryotic translation init... NM_001099661.2 99.8% 99.8% 871_873delGAG
6 human 728689 EIF3CL eukaryotic translation init... NM_001317856.1 99.8% 99.8% 871_873delGAG
7 human 728689 EIF3CL eukaryotic translation init... NM_001317857.1 99.8% 99.8% 871_873delGAG
8 human 8663 EIF3C eukaryotic translation init... NM_001286478.1 98.9% 98.9% 355_356ins30
9 human 8663 EIF3C eukaryotic translation init... XM_017023814.2 92.8% 91.4% (many diffs)
10 human 728689 EIF3CL eukaryotic translation init... XM_017023620.2 92.7% 91.3% (many diffs)
11 human 728689 EIF3CL eukaryotic translation init... XM_017023621.1 65.3% 65.3% 0_1ins948
12 mouse 56347 Eif3c eukaryotic translation init... NM_146200.1 89% 96.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2805
ORF length:
2739
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc gcggtttttc accaccggtt cggacagcga gtccgagtcg tccttgtccg
121gggaggagct cgtcaccaaa cctgtcggag gcaactatgg caaacagcca ttgttgctga
181gcgaggatga agaagatacc aagagagttg tccgcagtgc caaggacaag aggtttgagg
241agctgaccaa ccttatccgg accatccgta atgccatgaa gattcgtgat gtcaccaagt
301gcctggaaga gtttgagctc ctgggaaaag catatgggaa ggccaaaagc attgtggaca
361aagaaggtgt cccccggttc tatatccgca tcctggctga cctagaggac tatcttaatg
421agctttggga agataaggaa gggaagaaga agatgaacaa gaacaatgcc aaggctctga
481gcaccttgcg tcagaagatc cgaaaataca accgtgattt cgagtcccat atcacaagct
541acaagcagaa ccccgagcag tctgcggatg aagatgctga gaaaaatgag gaggattcag
601aaggctcttc agatgaggat gaggatgagg acggagtcag tgctgcaact ttcttgaaga
661agaaatcaga agctccttct ggggagagtc gcaagttcct caaaaagatg gatgatgaag
721atgaggactc agaagattcc gaagatgatg aagactggga cacaggttcc acatcttccg
781attccgactc agaggaggaa gaagggaaac aaaccgcgct ggcctcaaga tttcttaaaa
841aggcacccac cacagatgag gacaagaagg cagccgagaa gaaacgggag gacaaagcta
901agaagaagca cgacaggaaa tccaagcgcc tggatgagga ggaggaggac aatgaaggcg
961gggagtggga aagggtccgg ggcggagtgc cgttggttaa ggagaagcca aaaatgtttg
1021ccaagggaac tgagatcacc catgctgttg ttatcaagaa actgaatgag atcctacagg
1081cacgaggcaa gaagggaact gatcgtgctg cccagattga gctgctgcaa ctgctggttc
1141agattgcagc ggaaaacaac ctgggagagg gcgtcattgt caagatcaag ttcaatatca
1201tcgcctctct ctatgactac aaccccaacc tggcaaccta catgaagcca gagatgtggg
1261ggaagtgcct ggactgcatc aatgagctga tggatatcct gtttgcaaat cccaacattt
1321ttgttggaga gaatattctg gaagagagtg agaacctgca caacgctgac cagccactgc
1381gtgtccgtgg ctgcatccta actctggtgg aacgaatgga tgaagaattt accaaaataa
1441tgcaaaatac tgaccctcac tcccaagagt acgtggagca cttgaaggat gaggcccagg
1501tgtgtgccat catcgagcgt gtgcagcgct acctggagga gaagggcact accgaggagg
1561tctgccgcat ctacctgctg cgcatcctgc acacctacta caagtttgat tacaaggccc
1621atcagcgaca gctgaccccg cctgagggct cctcaaagtc tgagcaagac caggcagaaa
1681atgagggcga ggactcggct gtgttgatgg agagactgtg caagtacatc tacgccaagg
1741accgcacaga ccggatccgc acatgtgcca tcctctgcca catctaccac catgctctgc
1801actcgcgctg gtaccaggcc cgcgacctca tgctcatgag ccacttgcag gacaacattc
1861agcatgcaga cccgccagtg cagatccttt acaaccgcac catggtgcag ctgggcatct
1921gtgccttccg ccaaggcctg accaaggacg cacacaacgc cctgctggac atccagtcga
1981gtggccgagc caaggagctt ctgggccagg gcctgctgct gcgcagcctg caggagcgca
2041accaggagca ggagaaggtg gagcggcgcc gtcaggtccc cttccacctg cacatcaacc
2101tggagctgct ggagtgtgtc tacctggtgt ctgccatgct cctggagatc ccctacatgg
2161ccgcccatga gagcgatgcc cgccgacgca tgatcagcaa gcagttccac caccagctgc
2221gcgtgggcga gcgacagccc ctgctgggtc cccctgagtc catgcgggaa catgtggtcg
2281ctgcctccaa ggccatgaag atgggtgact ggaagacctg tcacagtttt atcatcaatg
2341agaagatgaa tgggaaagtg tgggaccttt tccccgaggc tgacaaagtc cgcaccatgc
2401tggttaggaa gatccaggaa gagtcactga ggacctacct cttcacctac agcagtgtct
2461atgactccat cagcatggag acgctgtcag acatgttTGA GCTGGATCTG CCCACTGTGC
2521ACTCCATCAT CAGCAAAATG ATCATTAATG AGGAGCTGAT GGCCTCCCTG GACCAGCCAA
2581CACAGACAGT GGTGATGCAC CGCACTGAGC CCACTGCCCA GCAGAACCTG GCTCTGCAGC
2641TGGCCGAGAA GCTGGGCAGC CTGGTGGAGA ACAACGAACG GGTGTTTGAC CACAAGCAGG
2701GCACCTACGG GGGCTACTTC CGAGACCAGA AGGACGGCTA CCGCAAAAAC GAGGGCTACA
2761TGCGCCGCGG TGGCTACCGC CAGCAGCAGT CTCAGACGGC CTACTGCCCA ACTTTCTTGT
2821ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
2881AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
2941GAAAGGACGA GTAAGTACAC CCCTAATCTC TATTACGCGT TAAGTCgaca atcaacctct
3001ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)