Construct: ORF TRCN0000478431
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004883.1_s317c1
- Derived from:
- ccsbBroadEn_01982
- DNA Barcode:
- GTAAGTACACCCCTAATCTCTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF3C (8663)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478431
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8663 | EIF3C | eukaryotic translation init... | NM_001037808.2 | 100% | 100% | |
| 2 | human | 8663 | EIF3C | eukaryotic translation init... | NM_001199142.1 | 100% | 100% | |
| 3 | human | 8663 | EIF3C | eukaryotic translation init... | NM_001267574.2 | 100% | 100% | |
| 4 | human | 8663 | EIF3C | eukaryotic translation init... | NM_003752.4 | 100% | 100% | |
| 5 | human | 728689 | EIF3CL | eukaryotic translation init... | NM_001099661.2 | 99.8% | 99.8% | 871_873delGAG |
| 6 | human | 728689 | EIF3CL | eukaryotic translation init... | NM_001317856.1 | 99.8% | 99.8% | 871_873delGAG |
| 7 | human | 728689 | EIF3CL | eukaryotic translation init... | NM_001317857.1 | 99.8% | 99.8% | 871_873delGAG |
| 8 | human | 8663 | EIF3C | eukaryotic translation init... | NM_001286478.1 | 98.9% | 98.9% | 355_356ins30 |
| 9 | human | 8663 | EIF3C | eukaryotic translation init... | XM_017023814.2 | 92.8% | 91.4% | (many diffs) |
| 10 | human | 728689 | EIF3CL | eukaryotic translation init... | XM_017023620.2 | 92.7% | 91.3% | (many diffs) |
| 11 | human | 728689 | EIF3CL | eukaryotic translation init... | XM_017023621.1 | 65.3% | 65.3% | 0_1ins948 |
| 12 | mouse | 56347 | Eif3c | eukaryotic translation init... | NM_146200.1 | 89% | 96.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2805
- ORF length:
- 2739
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gcggtttttc accaccggtt cggacagcga gtccgagtcg tccttgtccg 121 gggaggagct cgtcaccaaa cctgtcggag gcaactatgg caaacagcca ttgttgctga 181 gcgaggatga agaagatacc aagagagttg tccgcagtgc caaggacaag aggtttgagg 241 agctgaccaa ccttatccgg accatccgta atgccatgaa gattcgtgat gtcaccaagt 301 gcctggaaga gtttgagctc ctgggaaaag catatgggaa ggccaaaagc attgtggaca 361 aagaaggtgt cccccggttc tatatccgca tcctggctga cctagaggac tatcttaatg 421 agctttggga agataaggaa gggaagaaga agatgaacaa gaacaatgcc aaggctctga 481 gcaccttgcg tcagaagatc cgaaaataca accgtgattt cgagtcccat atcacaagct 541 acaagcagaa ccccgagcag tctgcggatg aagatgctga gaaaaatgag gaggattcag 601 aaggctcttc agatgaggat gaggatgagg acggagtcag tgctgcaact ttcttgaaga 661 agaaatcaga agctccttct ggggagagtc gcaagttcct caaaaagatg gatgatgaag 721 atgaggactc agaagattcc gaagatgatg aagactggga cacaggttcc acatcttccg 781 attccgactc agaggaggaa gaagggaaac aaaccgcgct ggcctcaaga tttcttaaaa 841 aggcacccac cacagatgag gacaagaagg cagccgagaa gaaacgggag gacaaagcta 901 agaagaagca cgacaggaaa tccaagcgcc tggatgagga ggaggaggac aatgaaggcg 961 gggagtggga aagggtccgg ggcggagtgc cgttggttaa ggagaagcca aaaatgtttg 1021 ccaagggaac tgagatcacc catgctgttg ttatcaagaa actgaatgag atcctacagg 1081 cacgaggcaa gaagggaact gatcgtgctg cccagattga gctgctgcaa ctgctggttc 1141 agattgcagc ggaaaacaac ctgggagagg gcgtcattgt caagatcaag ttcaatatca 1201 tcgcctctct ctatgactac aaccccaacc tggcaaccta catgaagcca gagatgtggg 1261 ggaagtgcct ggactgcatc aatgagctga tggatatcct gtttgcaaat cccaacattt 1321 ttgttggaga gaatattctg gaagagagtg agaacctgca caacgctgac cagccactgc 1381 gtgtccgtgg ctgcatccta actctggtgg aacgaatgga tgaagaattt accaaaataa 1441 tgcaaaatac tgaccctcac tcccaagagt acgtggagca cttgaaggat gaggcccagg 1501 tgtgtgccat catcgagcgt gtgcagcgct acctggagga gaagggcact accgaggagg 1561 tctgccgcat ctacctgctg cgcatcctgc acacctacta caagtttgat tacaaggccc 1621 atcagcgaca gctgaccccg cctgagggct cctcaaagtc tgagcaagac caggcagaaa 1681 atgagggcga ggactcggct gtgttgatgg agagactgtg caagtacatc tacgccaagg 1741 accgcacaga ccggatccgc acatgtgcca tcctctgcca catctaccac catgctctgc 1801 actcgcgctg gtaccaggcc cgcgacctca tgctcatgag ccacttgcag gacaacattc 1861 agcatgcaga cccgccagtg cagatccttt acaaccgcac catggtgcag ctgggcatct 1921 gtgccttccg ccaaggcctg accaaggacg cacacaacgc cctgctggac atccagtcga 1981 gtggccgagc caaggagctt ctgggccagg gcctgctgct gcgcagcctg caggagcgca 2041 accaggagca ggagaaggtg gagcggcgcc gtcaggtccc cttccacctg cacatcaacc 2101 tggagctgct ggagtgtgtc tacctggtgt ctgccatgct cctggagatc ccctacatgg 2161 ccgcccatga gagcgatgcc cgccgacgca tgatcagcaa gcagttccac caccagctgc 2221 gcgtgggcga gcgacagccc ctgctgggtc cccctgagtc catgcgggaa catgtggtcg 2281 ctgcctccaa ggccatgaag atgggtgact ggaagacctg tcacagtttt atcatcaatg 2341 agaagatgaa tgggaaagtg tgggaccttt tccccgaggc tgacaaagtc cgcaccatgc 2401 tggttaggaa gatccaggaa gagtcactga ggacctacct cttcacctac agcagtgtct 2461 atgactccat cagcatggag acgctgtcag acatgttTGA GCTGGATCTG CCCACTGTGC 2521 ACTCCATCAT CAGCAAAATG ATCATTAATG AGGAGCTGAT GGCCTCCCTG GACCAGCCAA 2581 CACAGACAGT GGTGATGCAC CGCACTGAGC CCACTGCCCA GCAGAACCTG GCTCTGCAGC 2641 TGGCCGAGAA GCTGGGCAGC CTGGTGGAGA ACAACGAACG GGTGTTTGAC CACAAGCAGG 2701 GCACCTACGG GGGCTACTTC CGAGACCAGA AGGACGGCTA CCGCAAAAAC GAGGGCTACA 2761 TGCGCCGCGG TGGCTACCGC CAGCAGCAGT CTCAGACGGC CTACTGCCCA ACTTTCTTGT 2821 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2881 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2941 GAAAGGACGA GTAAGTACAC CCCTAATCTC TATTACGCGT TAAGTCgaca atcaacctct 3001 ggattacaaa atttgtgaaa gatt