Construct: ORF TRCN0000478432
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003848.1_s317c1
- Derived from:
- ccsbBroadEn_13354
- DNA Barcode:
- ACTTAGCTGCTCTTCCCATTTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OLFML2A (169611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478432
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 169611 | OLFML2A | olfactomedin like 2A | NM_001282715.1 | 99.9% | 99.7% | 283A>G |
| 2 | human | 169611 | OLFML2A | olfactomedin like 2A | XM_006716989.2 | 69.9% | 69.8% | (many diffs) |
| 3 | human | 169611 | OLFML2A | olfactomedin like 2A | NM_182487.4 | 66% | 65.9% | (many diffs) |
| 4 | human | 169611 | OLFML2A | olfactomedin like 2A | XM_005251760.5 | 53.4% | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1380
- ORF length:
- 1314
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag taagagagac aaggctggga aggacacagc tagaggaaaa ggcaaggaca 121 tcagcaagta tggcagtgtg cagaaaagct ttgcagacag aggcctccca aaacctccca 181 aggagaagct gcttcaggtg gagaagctga gaaaggagag cggcaagggc agtttcctcc 241 agcccacagc caagccccgc gccctggccc agcagcaggc tgtgatccgg ggcttcacct 301 actacaaggc aggcaagcag gaggtgaccg aggcggtggc agacaacgcc ctccagggca 361 cttcctggct ggagcaactg ccgcccaagg tggagggcag gtccaactcc gcagagccca 421 actccgcaga gcaggatgag gctgagccca ggtcctccga gcgagtggac ctggcttctg 481 gcacccccac ttcaatccct gccaccacca ccaccgccac caccacccca acccccacca 541 ccagtctcct gcccaccgag ccaccttcag gtccagaagt ctccagccaa ggcagagagg 601 cgagctgtga gggcaccctc cgggctgtgg acccccctgt gaggcaccac agctatgggc 661 gccacgaggg agcctggatg aaggaccctg cagctcgaga cgacaggatc tatgtcacca 721 actactacta tggaaacagc ctggtggagt tccgcaacct ggaaaacttc aagcaaggcc 781 gctggagtaa catgtacaag ctaccctaca actggatcgg cacaggccac gtggtgtacc 841 agggcgcctt ctactacaac cgcgccttca ccaagaacat catcaagtac gacctacggc 901 agcgcttcgt ggcctcctgg gcgctgctgc ccgacgtggt atatgaggac accacacctt 961 ggaagtggcg cggacactcg gacattgact ttgccgtgga cgagagcggc ctgtgggtca 1021 tctaccccgc cgtggacgac cgcgatgagg cccagcccga ggtgatcgtc cTGAGTCGCT 1081 TGGACCCCGG CGATCTCTCC GTGCACCGGG AGACCACGTG GAAGACACGG CTGCGGCGGA 1141 ACTCCTACGG GAACTGCTTC CTGGTGTGCG GCATCCTGTA TGCCGTGGAC ACGTACAACC 1201 AGCAGGAAGG CCAGGTCGCC TACGCTTTCG ACACGCACAC GGGCACCGAC GCACGCCCCC 1261 AGCTGCCGTT CCTCAACGAG CACGCCTACA CCACCCAGAT CGACTACAAC CCCAAGGAGC 1321 GGGTGCTGTA CGCCTGGGAC AATGGCCACC AGCTCACCTA CACCCTCCAC TTCGTGGTCT 1381 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1441 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1501 TTTATATATC TTGTGGAAAG GACGAACTTA GCTGCTCTTC CCATTTTTAA CGCGTTAAGT 1561 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt