Construct: ORF TRCN0000478444
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010031.1_s317c1
- Derived from:
- ccsbBroadEn_09099
- DNA Barcode:
- TGAAAACAAATATATACCATCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX59 (83479)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478444
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83479 | DDX59 | DEAD-box helicase 59 | NM_001349799.2 | 99.8% | 99.8% | 663A>G;1416C>A |
| 2 | human | 83479 | DDX59 | DEAD-box helicase 59 | NM_001349800.2 | 99.8% | 99.8% | 663A>G;1416C>A |
| 3 | human | 83479 | DDX59 | DEAD-box helicase 59 | NM_001031725.6 | 99.8% | 99.8% | 663A>G;1416C>A;1431A>G |
| 4 | human | 83479 | DDX59 | DEAD-box helicase 59 | XM_017002432.2 | 99.8% | 99.8% | 663A>G;1416C>A;1431A>G |
| 5 | human | 83479 | DDX59 | DEAD-box helicase 59 | NM_001349801.2 | 92.9% | 92.8% | 663A>G;1416C>A;1465_1466ins129 |
| 6 | human | 83479 | DDX59 | DEAD-box helicase 59 | NM_001320181.2 | 88.7% | 86% | (many diffs) |
| 7 | human | 83479 | DDX59 | DEAD-box helicase 59 | NM_001349802.3 | 87.3% | 85.4% | (many diffs) |
| 8 | human | 83479 | DDX59 | DEAD-box helicase 59 | NM_001349803.2 | 86.3% | 86.2% | 663A>G;1062_1063ins252;1164C>A |
| 9 | human | 83479 | DDX59 | DEAD-box helicase 59 | NM_001320182.1 | 81.4% | 81.4% | 663A>G;972_973ins342;1074C>A |
| 10 | human | 83479 | DDX59 | DEAD-box helicase 59 | XR_921967.3 | 77.2% | (many diffs) | |
| 11 | human | 83479 | DDX59 | DEAD-box helicase 59 | XR_921966.2 | 76% | (many diffs) | |
| 12 | human | 83479 | DDX59 | DEAD-box helicase 59 | XR_001737463.2 | 71.9% | (many diffs) | |
| 13 | human | 83479 | DDX59 | DEAD-box helicase 59 | NM_001349804.1 | 71.1% | 71.2% | 663A>G;1062_1063ins534 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1923
- ORF length:
- 1857
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tgttccaaga tctctaaaaa tcaagaggaa tgctaatgat gatggcaaaa 121 gttgtgtggc taagataatt aaaccagacc cagaagacct tcagttggac aaaagcagag 181 atgttcccgt tgatgctgta gctacagaag cagccacaat agacaggcac atcagcgaat 241 catgcccttt ccccagccca ggtggccagt tggcagaggt tcattcagta agtcccgagc 301 agggtgcgaa ggacagccat ccttctgaag agcccgttaa gtcattttcc aaaacacagc 361 gctgggcaga accaggggaa cccatctgtg ttgtctgtgg tcgttatgga gagtatatct 421 gtgataagac agatgaagat gtgtgtagtt tggagtgtaa agcgaaacat cttctacaag 481 ttaaggaaaa ggaagagaaa tcaaaactca gcaatccaca gaaggctgat tctgagccag 541 agtctccact gaatgcttcc tatgtctaca aagagcaccc ctttattttg aaccttcagg 601 aagaccagat tgaaaatctt aaacagcagc tgggaatttt agttcaaggg caagaagtca 661 ccaggcccat tattgacttt gaacattgta gtctccctga ggtcttaaat cacaacttga 721 agaaatcggg ctatgaggtg ccaactccca ttcaaatgca gatgattcct gtgggacttc 781 tgggaagaga cattctggcc agtgcagata ctggctcagg aaaaacagct gcttttcttc 841 ttcctgttat catgcgagct ttattcgaga gcaaaactcc atctgcgctc attcttacac 901 caaccagaga gttagccatt cagatagaga gacaagctaa agaattgatg agtggcctgc 961 cacgcatgaa aactgtgctt cttgtagggg gcttaccctt acccccacag ctttatcgtc 1021 tgcaacaaca tgttaaggtt atcatagcaa cccctgggcg acttctggat ataataaagc 1081 agagctctgt agaactctgt ggtgtaaaga ttgtggtagt agatgaagct gataccatgt 1141 taaagatggg ttttcaacaa caagtgcttg acattttgga aaacattcct aatgattgtc 1201 agaccatttt ggtttcagcc acaattccaa ctagcataga acagctagca agccagcttc 1261 tgcataatcc tgtgagaatt atcactggag aaaagaacct accttgtgcc aatgtacgtc 1321 agattatttt gtgggtagaa gacccagcca aaaagaaaaa attatttgaa attttaaatg 1381 ataagaaact ctttaagcct ccagtgttag tatttgtgga ctgcaaacta ggagcagatc 1441 ttttgagtga agccgttcag aaaatcacag ggctgaaaag aatatctata cattcggaga 1501 agtcgcaaat agaaaggaaa aacatattga agggattact tgaaggagac tatGAAGTTG 1561 TAGTGAGCAC AGGAGTCTTG GGACGAGGCC TAGACTTGAT CAGTGTCAGG CTGGTTGTCA 1621 ATTTTGATAT GCCTTCAAGT ATGGATGAGT ATGTCCATCA GATTGGAAGA GTAGGAAGAT 1681 TAGGTCAAAA TGGAACAGCG ATTACTTTCA TCAATAATAA TTCAAAAAGA CTCTTCTGGG 1741 ATATTGCAAA ACGAGTAAAG CCCACAGGAT CCATTCTTCC CCCTCAGTTA TTAAATTCCC 1801 CATACCTTCA TGACCAGAAG AGAAAGGAAC AACAGAAAGA TAAACAGACA CAGAATGATC 1861 TGGTTACAGG AGCTAATCTT ATGGATATTA TCAGAAAACA TGATAAAAGT AATTCTCAGA 1921 AATGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1981 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2041 GGCTTTATAT ATCTTGTGGA AAGGACGATG AAAACAAATA TATACCATCA TCACGCGTTA 2101 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt