Construct: ORF TRCN0000478448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012487.2_s317c1
- Derived from:
- ccsbBroadEn_06726
- DNA Barcode:
- AATATGCTTTTCCAGCTTGTATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIGF (5281)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478448
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5281 | PIGF | phosphatidylinositol glycan... | NM_173074.3 | 99.8% | 100% | 327A>G |
| 2 | human | 5281 | PIGF | phosphatidylinositol glycan... | XM_005264369.3 | 89.8% | 88.8% | (many diffs) |
| 3 | human | 5281 | PIGF | phosphatidylinositol glycan... | XM_011532908.3 | 87.5% | 81.1% | (many diffs) |
| 4 | human | 5281 | PIGF | phosphatidylinositol glycan... | NM_002643.4 | 85.5% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 684
- ORF length:
- 618
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa agataacgat atcaagagac tactgtatac ccatctttta tgcatatttt 121 caattatcct aagtgtcttc attccatcac tcttcttgga gaacttctca atattggaaa 181 cacacttgac atggttgtgc atctgttctg gttttgtaac tgctgtcaat ctagtactat 241 atttagtagt gaaaccaaat acatcctcta aaagaagttc attatcacac aaggtaactg 301 gatttttgaa atgctgtatc tactttctta tgtcttgttt ctcctttcat gtaatttttg 361 ttctgtatgg agcacCACTG ATAGAGTTGG CGTTGGAAAC ATTTTTATTT GCAGTTATTT 421 TGTCTACTTT TACTACTGTG CCTTGCTTAT GTTTGTTAGG ACCAAACCTC AAAGCATGGC 481 TAAGAGTGTT CAGTAGAAAT GGAGTTACAT CCATATGGGA GAATAGTCTC CAGATCACTA 541 CAATTTCTAG CTTTGTAGGA GCATGGCTTG GAGCACTTCC TATTCCACTG GATTGGGAAA 601 GACCATGGCA GATGACAGTA GAAAGAAAAC GTAGCACTTA TAGATCTCTC CATGTACCCT 661 GCAGGGGGCT TGGTACTGTG AAATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 721 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 781 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ATATGCTTTT 841 CCAGCTTGTA TGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 901 att